The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Chemical Compound Review

Cimagel     2-dodecoxyethanol

Synonyms: Laureth, Thesat, Thesit, atoxysclerol, Dodecylglycol, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Laureth


Psychiatry related information on Laureth


High impact information on Laureth

  • The non-stimulated (basal) adenylate cyclase from bovine brain cortical membranes was purified 10 000-fold to apparent homogeneity by Lubrol PX extraction and two cycles of affinity chromatography on forskolin-agarose [7].
  • Neutrophils treated with the nonionic detergent Lubrol PX, under controlled conditions, yield intact detergent-insoluble ghosts [8].
  • The protein could be extracted in an active form by rupturing the cells with lysozyme and sonication or with a passage through a French pressure cell and incubating the inclusion bodies and membranous structures with detergent (Lubrol PX or deoxycholate) in the presence of Q or S Sepharose ion-exchange resin for several hours [9].
  • An apparent receptor for the egg peptide speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly) was identified by covalently coupling a radiolabeled speract analogue to intact spermatozoa and was then purified by DEAE-Sepharose chromatography and preparative gel electrophoresis after solubilization with Lubrol PX [10].
  • Measurement of the Stokes radius of p61 and p64 by gel filtration shows that they are not physically associated in buffer containing the nonionic detergent Lubrol 12A9 [11].

Chemical compound and disease context of Laureth


Biological context of Laureth

  • 5) Lubrol 12A9 potently inhibited the GTPase reaction and the dissociation of Gs-GDP in parallel, and inhibition of product release may account for the inhibition of steady-state hydrolysis [16].
  • The strength of binding between the porphyrins and Brij 35 depends on the number of binding sites and the solvation shell [17].
  • It inhibited PP1 and PP2A enzymatic activity concentration-dependently with IC50 values of 20 and 75 pM, respectively, in the presence of 0.01% Brij-35, and an LC50 value of 1 microM [18].
  • Influence of the nonionic surfactant Brij 35 on the bioavailability of solid and sorbed dibenzofuran [19].
  • The ligand sito-G was confirmed to be able to enhance ASGPR-mediated endocytosis, the nonionic surfactant Brij 35 seemed to be able to facilitate membrane fusion, and the co-modification resulted in the most efficient transfection but no enhanced cytotoxicity [20].

Anatomical context of Laureth

  • Treatment of the isolated bovine granules with 0.2% Lubrol PX results in the solubilization of most membranes present in the fractin but has only a limited effect on the matrices, which remain aggregated and can be recovered and purified by gradient centrifugation [21].
  • Toxin-activated cyclase activity of fat cell membranes is retained upon solubilization with Lubrol PX [22].
  • C. tropicalis was grown in a medium containing Brij 35, resulting in the induction of a moderate number of medium-sized peroxisomes [23].
  • Association of LFA-1 with lipid rafts of primary T cells could be detected only when they were isolated with another nonionic detergent, Brij 35 [24].
  • SITS-Affi-Gel 102 binds proteins extracted from the porcine cauda epididymal sperm plasma membrane by Lubrol-PX, more selectively than heparin-agarose [25].

Associations of Laureth with other chemical compounds

  • The sedimentation properties of the solubilized receptor-hormone complexes formed in vivo were identical with those derived for the corresponding complexes formed in vitro and extracted with Triton X-100 and Lubrol PX, with sedimentation constants of 8.8 S for the Triton-solubilized complex and 7.0 S for the complex extracted with Lubrol PX [26].
  • The guanosine 5'-[beta, gamma-imido]triphosphate (p[NH]ppG)-activated adenylate cyclase from rabbit myocardial membranes was purified approximately equal to 60,000-fold to a specific activity of 15 mumol X mg-1 X min-1 by Lubrol PX extraction, affinity chromatography, and gel permeation HPLC [27].
  • The Ca-ATPase of sarcoplasmic reticulum was solubilized at pH 6.5 and 30 degrees C using different nonionic detergents, Triton X-100, C12E8, Lubrol PX, or Tween 20 [28].
  • Retinol dehydrogenase solubilized by Lubrol 12A9 from bovine retinal rod outer segments forms mixed micelles of Stokes radius 8.5 nm [29].
  • The activated propranolol-resistant state persisted following various treatments of the enzyme preparation including extensive washings of the membranes; considerable activity was retained even after sonication or treatment with the detergent Lubrol-PX, treatments which caused inactivation of the native enzyme [30].

Gene context of Laureth

  • In Brij 35 lysates, FcR gamma-chain was found to constitutively associate with GPIb [31].
  • From the membrane fraction of cultured human renal adenocarcinoma (ACHN) cells, two endothelin-2-converting enzymes (ECE-2A and ECE-2B) were solubilized with detergent Lubrol PX and separated by hydrophobic butyl fast-performance liquid chromatography [32].
  • This assay used methoxysuccinyl-dialanine-proline-valine-p-nitroanilide as substrate in the presence of 0.01% Brij 35, an HLE enzyme activator [33].
  • Two forms of tyrosinase from B16 mouse melanoma were identified by nonreducing SDS-PAGE after solubilization of crude melanosomal preparations with the nonionic detergent Brij 35 [34].
  • The presence of nonionic detergents of the polyoxyethylene type such as Tween 20, Triton X-100, Nonidet P40 or Lubrol PX in the incubation buffer was necessary to induce renaturation of blotted recombinant c-Ha-ras protein, whereas other types of detergents were ineffective [35].

Analytical, diagnostic and therapeutic context of Laureth

  • After sensitization with IgG, the integral membrane proteins were solubilized in Brij 36T nonionic detergent and chromatographed by gel filtration [36].
  • Partially purified MDHV-B was analyzed by sucrose gradient sedimentation, isoelectric focusing, and gel filtration on Sephadex G-200 in the presence of 1.0 or 2.0 M urea and 0.05% Brij 35 to purify the antigen and to determine its physical and chemical properties in comparison with those already reported for MDHV-A [37].
  • The optimum procedure includes a derivatization step of the herbicides at 40 degrees C with DTAF for 1 h and a 2-fold dilution prior to MEKC analysis, which is conducted within about 10 min using Brij-35 in the running buffer [38].
  • In the presence of 0.05% Lubrol PX, its predicted secondary structure from circular dichroism spectroscopy is 1% alpha-helix, 21% beta-sheet, and 78% random coil [39].
  • Enzyme activity was extracted from a membrane fraction of human diencephalon with a non-ionic detergent, Brij 35, and activity was monitored by measuring the disappearance of added substance P using radioimmunoassay, bioassay or radiochemical assay [40].


  1. Characterization of the receptor for heat-stable enterotoxin from Escherichia coli in rat intestine. Kuno, T., Kamisaki, Y., Waldman, S.A., Gariepy, J., Schoolnik, G., Murad, F. J. Biol. Chem. (1986) [Pubmed]
  2. Structural analysis of the hepatic glucagon receptor. Identification of a guanine nucleotide-sensitive hormone-binding region. Iyengar, R., Herberg, J.T. J. Biol. Chem. (1984) [Pubmed]
  3. Guanylate cyclase and cyclic guanosine 3':5'-monophosphate phosphodiesterase activities and cyclic guanosine 3':5'-monophosphate levels in normal and transformed fibroblasts in culture. Nesbitt, J.A., Anderson, W.B., Miller, Z., Pastan, I., Russell, T.R., Gospodarowicz, D. J. Biol. Chem. (1976) [Pubmed]
  4. Resolution of some components of adenylate cyclase necessary for catalytic activity. Ross, E.M., Gilman, A.G. J. Biol. Chem. (1977) [Pubmed]
  5. A synthetic conformational epitope from the C4 domain of HIV Gp120 that binds CD4. Robey, F.A., Harris-Kelson, T., Robert-Guroff, M., Batinić, D., Ivanov, B., Lewis, M.S., Roller, P.P. J. Biol. Chem. (1996) [Pubmed]
  6. A comparison of dye binding methods for albumin determination: the effects of abnormal sera, reaction times, acute phase reactants and albumin standards. Walsh, R.L. Clin. Biochem. (1983) [Pubmed]
  7. Adenylate cyclase from bovine brain cortex: purification and characterization of the catalytic unit. Pfeuffer, E., Mollner, S., Pfeuffer, T. EMBO J. (1985) [Pubmed]
  8. Transmembrane linkage between surface glycoproteins and components of the cytoplasm in neutrophil leukocytes. Sheterline, P., Hopkins, C.R. J. Cell Biol. (1981) [Pubmed]
  9. Regulated high-level expression of the mannitol permease of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli. van Weeghel, R.P., Keck, W., Robillard, G.T. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  10. Cloning of the mRNA for the protein that crosslinks to the egg peptide speract. Dangott, L.J., Jordan, J.E., Bellet, R.A., Garbers, D.L. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  11. Partial purification and characterization of a pp60v-src-related tyrosine kinase from bovine brain. Neer, E.J., Lok, J.M. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  12. Allergic contact dermatitis from tertiary-butylhydroquinone and Laureth 12 in a hair dye. Field, S., Hazelwood, E., Bourke, B., Bourke, J.F. Contact Derm. (2007) [Pubmed]
  13. Effect of introduced phthalate-degrading bacteria on the diversity of indigenous bacterial communities during di-(2-ethylhexyl) phthalate (DEHP) degradation in a soil microcosm. Chao, W.L., Cheng, C.Y. Chemosphere (2007) [Pubmed]
  14. Solubilization and characterization of the partially purified penicillin sensitive D-alanine carboxypeptidase of Neisseria gonorrhoeae. Davis, R.H., Linder, R., Salton, M.R. Microbios (1978) [Pubmed]
  15. Etoposide (VP-16) uptake by tumour spheroids and activity in the presence of Brij 30, formulation additives and sodium salicylate. Cook, J.M., Florence, A.T., Russell, J., Wheldon, T.E. European journal of cancer & clinical oncology. (1989) [Pubmed]
  16. GTPase activity of the stimulatory GTP-binding regulatory protein of adenylate cyclase, Gs. Accumulation and turnover of enzyme-nucleotide intermediates. Brandt, D.R., Ross, E.M. J. Biol. Chem. (1985) [Pubmed]
  17. Quantitative description of migration behavior of porphyrins based on the dynamic complexation model in a nonaqueous capillary electrophoresis system. Bowser, M.T., Sternberg, E.D., Chen, D.D. Electrophoresis (1997) [Pubmed]
  18. Different moieties of tautomycin involved in protein phosphatase inhibition and induction of apoptosis. Kawamura, T., Matsuzawa, S., Mizuno, Y., Kikuchi, K., Oikawa, H., Oikawa, M., Ubukata, M., Ichihara, A. Biochem. Pharmacol. (1998) [Pubmed]
  19. Influence of the nonionic surfactant Brij 35 on the bioavailability of solid and sorbed dibenzofuran. Garcia, J.M., Wick, L.Y., Harms, H. Environ. Sci. Technol. (2001) [Pubmed]
  20. Mechanisms of co-modified liver-targeting liposomes as gene delivery carriers based on cellular uptake and antigens inhibition effect. Zhang, Y., Rong Qi, X., Gao, Y., Wei, L., Maitani, Y., Nagai, T. Journal of controlled release : official journal of the Controlled Release Society (2007) [Pubmed]
  21. Molecular organization of prolactin granules. II. Characterization of glycosaminoglycans and glycoproteins of the bovine prolactin matrix. Zanini, A., Giannattasio, G., Nussdorfer, G., Margolis, R.K., Margolis, R.U., Meldolesi, J. J. Cell Biol. (1980) [Pubmed]
  22. Mechanism of action of cholera toxin and the mobile receptor theory of hormone receptor-adenylate cyclase interactions. Bennett, V., O'Keefe, E., Cuatrecasaş, P. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  23. Efficient association of in vitro translation products with purified stable Candida tropicalis peroxisomes. Small, G.M., Imanaka, T., Shio, H., Lazarow, P.B. Mol. Cell. Biol. (1987) [Pubmed]
  24. Membrane cholesterol regulates LFA-1 function and lipid raft heterogeneity. Marwali, M.R., Rey-Ladino, J., Dreolini, L., Shaw, D., Takei, F. Blood (2003) [Pubmed]
  25. Purification of bicarbonate-sensitive sperm adenylylcyclase by 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid-affinity chromatography. Okamura, N., Tajima, Y., Onoe, S., Sugita, Y. J. Biol. Chem. (1991) [Pubmed]
  26. Physical characteristics of the gonadotropin receptor-hormone complexes formed in vivo and in vitro. Dufau, M.L., Podesta, E.J., Catt, K.J. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  27. Catalytic unit of adenlyate cyclase: purification and identification by affinity crosslinking. Pfeuffer, E., Dreher, R.M., Metzger, H., Pfeuffer, T. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  28. Stability and partial reactions of soluble and membrane-bound sarcoplasmic reticulum ATPase. Martins, O.B., de Meis, L. J. Biol. Chem. (1985) [Pubmed]
  29. Retinol dehydrogenase from bovine retinal rod outer segments. Kinetic mechanism of the solubilized enzyme. Nicotra, C., Livrea, M.A. J. Biol. Chem. (1982) [Pubmed]
  30. A persistent active state of the adenylate cyclase system produced by the combined actions of isoproterenol and guanylyl imidodiphosphate in frog erythrocyte membranes. Schramm, M., Rodbell, M. J. Biol. Chem. (1975) [Pubmed]
  31. Role of Fc receptor gamma-chain in platelet glycoprotein Ib-mediated signaling. Wu, Y., Suzuki-Inoue, K., Satoh, K., Asazuma, N., Yatomi, Y., Berndt, M.C., Ozaki, Y. Blood (2001) [Pubmed]
  32. Endothelin-2-converting enzyme from human renal adenocarcinoma cells is a phosphoramidon-sensitive, membrane-bound metalloprotease. Shinmi, O., Yorimitsu, K., Moroi, K., Nishiyama, M., Sugita, Y., Saito, T., Inagaki, Y., Masaki, T., Kimura, S. J. Cardiovasc. Pharmacol. (1993) [Pubmed]
  33. A rapid procedure for the measurement of elastase inhibitory function of plasma and bronchoalveolar lavage fluid. Walsh, R.L., Dillon, T.J., McLennan, G. Clin. Biochem. (1992) [Pubmed]
  34. Tyrosinase isoenzymes: two melanosomal tyrosinases with different kinetic properties and susceptibility to inhibition by calcium. Jiménez-Cervantes, C., Solano, F., Lozano, J.A., Garcia-Borrón, J.C. Pigment Cell Res. (1994) [Pubmed]
  35. GTP-blot analysis of small GTP-binding proteins. The C-terminus is involved in renaturation of blotted proteins. Klinz, F.J. Eur. J. Biochem. (1994) [Pubmed]
  36. Anti-Rho(D) IgG binds to band 3 glycoprotein of the human erythrocyte membrane. Victoria, E.J., Mahan, L.C., Masouredis, S.P. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
  37. Marke's disease herpesviruses. III. Purification and characterization of Marek's disease herpesvirus B antigen. Velicer, L.F., Yager, D.R., Clark, J.L. J. Virol. (1978) [Pubmed]
  38. Analytical potential of fluorescein analogues for ultrasensitive determinations of phosphorus-containing amino acid herbicides by micellar electrokinetic chromatography with laser-induced fluorescence detection. Molina, M., Silva, M. Electrophoresis (2002) [Pubmed]
  39. Physicochemical and hydrodynamic characterization of P-57, a neurospecific calmodulin binding protein. Masure, H.R., Alexander, K.A., Wakim, B.T., Storm, D.R. Biochemistry (1986) [Pubmed]
  40. Purification and characterisation of a membrane-bound substance-P-degrading enzyme from human brain. Lee, C.M., Sandberg, B.E., Hanley, M.R., Iversen, L.L. Eur. J. Biochem. (1981) [Pubmed]
WikiGenes - Universities