Disease relevance of jar

• This reduction of 95F myosin causes male sterility as a result of defects in spermatid individualization [1].

Psychiatry related information on jar

• The motor activity of 95F myosin itself and/or components within the particles transported to the furrows by 95F myosin may be required for normal furrows to form [2].

High impact information on jar

• Altered patterns of beta 3-tubulin and myosin heavy chain gene expression in the mutants indicate a role for the neurogenic genes in development of most visceral and somatic muscles [3].
• Myosin VI, unlike almost all other myosins, moves toward the minus end of actin filaments and functions in a variety of intracellular processes such as vesicular membrane traffic, cell migration, and mitosis [4].
• The absence of other alternative exons in the rod-coding region, aside from those shown previously to encode alternative carboxyl termini, demonstrates that the bulk of the myosin rod is not involved in the generation of isoform-specific properties of the MHC molecule [5].
• As opposed to the null phenotype observed in the IFM, normal amounts of MHC accumulate in the leg muscles of three of these mutants, whereas the fourth mutant shows a 45% reduction in leg muscle MHC [6].
• The indirect flight muscles (IFMs) of each of these homozygous mutants fail to accumulate MHC, lack thick filaments, and do not display normal cylindrical myofibrils [6].

Biological context of jar

• Myosin VI is required for E-cadherin-mediated border cell migration [7].
• Asymmetric cell division: Miranda chauffeured by Jaguar [8]?
• 95F myosin and D-CLIP-190 are coexpressed in a number of tissues during embryogenesis in Drosophila [9].
• Germ line transformation with the 95F myosin heavy chain cDNA rescues the male sterility phenotype [1].
• Finally Acam binds with high affinity to peptide versions of both myosin VI light chain binding sites [10].

Anatomical context of jar

• An intact F-actin cytoskeleton was known to be required, and now a myosin VI (Jaguar) has been shown to be necessary for basal targeting of cell fate determinants in neuroblasts [8].
• In wild-type testes, 95F myosin localizes to the leading edge of the IC [1].
• Here, we demonstrate a new function of myosin VI using observations of Drosophila spermatid individualization in vivo [11].
• One of these alleles is characterized by unfused myoblasts which express beta 3 tubulin and myosin heavy chain, indicating the state of cell differentiation [12].
• Myosin VI is crucial for correct cell morphology and maintenance of adhesive cellular contacts within epithelial cell layers [13].

Associations of jar with chemical compounds

• A clone of a fast isoform of myosin heavy chain (HC) gene was isolated from a cDNA1 expression library made from mRNA purified from the deep abdominal flexor muscle of the lobster, Homarus americanus [14].

Other interactions of jar

• A role for myosin VI in actin dynamics at sites of membrane remodeling during Drosophila spermatogenesis [15].
• Androcam is a tissue-specific light chain for myosin VI in the Drosophila testis [10].
• Porcine myosin-VI is highly homologous to Drosophila 95F myosin heavy chain, and together these two myosins comprise a sixth class of myosin motors [16].

Analytical, diagnostic and therapeutic context of jar

• Further, myosin VI and Acam co-immunoprecipitate from the testis and interact in yeast two-hybrid assays [10].
• In this study, in situ hybridization analysis has been used to examine the complexity and muscle specificity of MHC isoform expression in the fibrillar indirect flight muscle (IFM), the tubular direct flight muscles (DFM) and tubular tergal depressor of the trochanter muscle (TDT), and the visceral esophageal muscle in the adult thorax [17].
• The quantity of MHC transcript was measured in embryos of various stages by single-strand RNA probe excess titration [18].
• We report here on the cis-regulatory mechanisms that direct the processing of alternative exons in Mhc exon 11 in individual muscles using transgenic reporter constructs, RT-PCR and directed mutagenesis [19].
• Video-intensified fluorescence timelapse microscopy, in combination with microinjections of antibodies directed against Drosophila 95F myosin, have revealed that this unconventional myosin of class VI is involved in the transport processes [20].

References