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Gene Review

RRM2B  -  ribonucleotide reductase M2 B (TP53...

Homo sapiens

Synonyms: MTDPS8A, MTDPS8B, P53R2, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, ...
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Disease relevance of RRM2B


High impact information on RRM2B

  • Our results indicate that p53R2 encodes a ribonucleotide reductase that is directly involved in the p53 checkpoint for repair of damaged DNA [5].
  • Induction of p53R2 in p53-deficient cells caused G2/M arrest and prevented cells from death in response to adriamycin [5].
  • Inhibition of endogenous p53R2 expression in cells that have an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair and cell survival after exposure to various genotoxins [5].
  • Mammalian two-hybrid assay further indicates that the amino acids 1 to 113 of p53R2 are critical for interacting with the NH(2)-terminal region (amino acids 1-93) of p21 [6].
  • These data suggest a new function of p53R2 of cooperating with p21 during DNA repair at G(1) arrest [6].

Chemical compound and disease context of RRM2B


Biological context of RRM2B

  • Screening of nine exons of the p53R2 [Human Genome Organisation (HUGO) official name RRM2B] gene resulted in identification of a novel polymorphism in the 5' untranslated region, which was detected in four cases [8].
  • The human ribonucleotide reductase subunit hRRM2 complements p53R2 in response to UV-induced DNA repair in cells with mutant p53 [9].
  • An increase in p53R2 expression by gene transfection significantly reduced the cellular invasion potential to 54% and 30% in KB and PC-3 cells, respectively, whereas inhibition of p53R2 by short interfering RNA resulted in a 3-fold increase in cell migration [1].
  • CONCLUSIONS: Opposite regulation of hRRM2 and p53R2 in invasion potential might play a critical role in determining the invasion and metastasis phenotype in cancer cells [1].
  • p53R2-dependent pathway for DNA synthesis in a p53-regulated cell cycle checkpoint [10].

Anatomical context of RRM2B


Associations of RRM2B with chemical compounds

  • To the contrary, p53R2 was 2.50-fold less sensitive than hRRM2 to the radical scavenger hydroxyurea, whereas EPR showed similar spectra of the tyrosyl radical in two proteins [12].
  • Triapine, a new RR inhibitor, was equally potent for p53R2 and hRRM2 [12].
  • Of interest, p53R2 was 158-fold more susceptible to the iron chelator deferoxamine mesylate than hRRM2, although the iron content of the two proteins determined by atomic absorption spectrometer was almost the same [12].
  • No significant change in the expression of RRM2 was observed in either cell line, although both gemcitabine-resistant cell lines had an approximate 3-fold increase in p53R2 protein [13].
  • Ectopic expression of p53R2 partially reversed the cytotoxicity of cisplatin but not that of RNR inhibitors to R2 knockdown cells [14].

Physical interactions of RRM2B

  • Our results suggest that wild-type p53 directly interacts with both p53R2 and hRRM2 [15].
  • These results suggest that PC3 cells are deficient in both transcription of p53R2 and binding to hRRM1 in response to UV irradiation [9].

Regulatory relationships of RRM2B

  • Wild-type p53 regulates human ribonucleotide reductase by protein-protein interaction with p53R2 as well as hRRM2 subunits [15].

Other interactions of RRM2B


Analytical, diagnostic and therapeutic context of RRM2B


  1. Metastasis-Suppressing Potential of Ribonucleotide Reductase Small Subunit p53R2 in Human Cancer Cells. Liu, X., Zhou, B., Xue, L., Shih, J., Tye, K., Lin, W., Qi, C., Chu, P., Un, F., Wen, W., Yen, Y. Clin. Cancer Res. (2006) [Pubmed]
  2. Expression of p53R2, newly p53 target in oral normal epithelium, epithelial dysplasia and squamous cell carcinoma. Yanamoto, S., Kawasaki, G., Yoshitomi, I., Mizuno, A. Cancer Lett. (2003) [Pubmed]
  3. Novel genetic variations of the p53R2 gene in patients with colorectal adenoma and controls. Deng, Z.L., Xie, D.W., Bostick, R.M., Miao, X.J., Gong, Y.L., Zhang, J.H., Wargovich, M.J. World J. Gastroenterol. (2005) [Pubmed]
  4. Effect of high-risk human papillomavirus oncoproteins on p53R2 gene expression after DNA damage. Lembo, D., Donalisio, M., Cornaglia, M., Azzimonti, B., Demurtas, A., Landolfo, S. Virus Res. (2006) [Pubmed]
  5. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Tanaka, H., Arakawa, H., Yamaguchi, T., Shiraishi, K., Fukuda, S., Matsui, K., Takei, Y., Nakamura, Y. Nature (2000) [Pubmed]
  6. Ribonucleotide Reductase Small Subunit p53R2 Facilitates p21 Induction of G1 Arrest under UV Irradiation. Xue, L., Zhou, B., Liu, X., Heung, Y., Chau, J., Chu, E., Li, S., Jiang, C., Un, F., Yen, Y. Cancer Res. (2007) [Pubmed]
  7. Silencing of the p53R2 gene by RNA interference inhibits growth and enhances 5-fluorouracil sensitivity of oral cancer cells. Yanamoto, S., Iwamoto, T., Kawasaki, G., Yoshitomi, I., Baba, N., Mizuno, A. Cancer Lett. (2005) [Pubmed]
  8. Genetic status of cell cycle regulators in squamous cell carcinoma of the oesophagus: the CDKN2A (p16(INK4a) and p14(ARF) ) and p53 genes are major targets for inactivation. Smeds, J., Berggren, P., Ma, X., Xu, Z., Hemminki, K., Kumar, R. Carcinogenesis (2002) [Pubmed]
  9. The human ribonucleotide reductase subunit hRRM2 complements p53R2 in response to UV-induced DNA repair in cells with mutant p53. Zhou, B., Liu, X., Mo, X., Xue, L., Darwish, D., Qiu, W., Shih, J., Hwu, E.B., Luh, F., Yen, Y. Cancer Res. (2003) [Pubmed]
  10. p53R2-dependent pathway for DNA synthesis in a p53-regulated cell cycle checkpoint. Yamaguchi, T., Matsuda, K., Sagiya, Y., Iwadate, M., Fujino, M.A., Nakamura, Y., Arakawa, H. Cancer Res. (2001) [Pubmed]
  11. Structurally dependent redox property of ribonucleotide reductase subunit p53R2. Xue, L., Zhou, B., Liu, X., Wang, T., Shih, J., Qi, C., Heung, Y., Yen, Y. Cancer Res. (2006) [Pubmed]
  12. In vitro characterization of enzymatic properties and inhibition of the p53R2 subunit of human ribonucleotide reductase. Shao, J., Zhou, B., Zhu, L., Qiu, W., Yuan, Y.C., Xi, B., Yen, Y. Cancer Res. (2004) [Pubmed]
  13. An increase in the expression of ribonucleotide reductase large subunit 1 is associated with gemcitabine resistance in non-small cell lung cancer cell lines. Davidson, J.D., Ma, L., Flagella, M., Geeganage, S., Gelbert, L.M., Slapak, C.A. Cancer Res. (2004) [Pubmed]
  14. Stable suppression of the R2 subunit of ribonucleotide reductase by R2-targeted short interference RNA sensitizes p53(-/-) HCT-116 colon cancer cells to DNA-damaging agents and ribonucleotide reductase inhibitors. Lin, Z.P., Belcourt, M.F., Cory, J.G., Sartorelli, A.C. J. Biol. Chem. (2004) [Pubmed]
  15. Wild-type p53 regulates human ribonucleotide reductase by protein-protein interaction with p53R2 as well as hRRM2 subunits. Xue, L., Zhou, B., Liu, X., Qiu, W., Jin, Z., Yen, Y. Cancer Res. (2003) [Pubmed]
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