Disease relevance of UBAP1

• Pure ALS, through ALS with cognitive impairment and ALS-FTD to pure FTD-U, may represent a continuous spectrum of ubiquitin-associated neurodegenerative disease [1].
• Structure of the ubiquitin-associated domain of p62 (SQSTM1) and implications for mutations that cause Paget's disease of bone [2].
• Platelet-rich plasma was prepared from the citrated blood of 12 patients with unstable angina (UAP) and 20 patients with acute myocardial infarction (AMI) who were admitted within 3 hours of the onset of cardiac symptoms and from 18 controls [3].
• METHODS: Thirty patients with unstable angina pectoris (UAP group) and 15 patients with acute myocardial infarction (MI group) were studied [4].
• PATIENTS: 390 patients with stable angina pectoris (SAP) and 306 with unstable angina (UAP) [5].

High impact information on UBAP1

• HHR23A, a protein implicated in nucleotide excision repair, belongs to a class of proteins containing both a ubiquitin-like (Ubl) domain and one or more ubiquitin-associated (UBA) domains, suggesting a role in the ubiquitin-proteasome pathway as well [6].
• The presence of UBA domains provides a possible link between DNA methylation and ubiquitin/proteasome pathways [7].
• Mutational analysis of the UBA domain reveals that the conserved hydrophobic patch MGF as well as the conserved leucine in helix 2 are necessary for binding polyubiquitinated proteins and for sequestration-aggregate formation [8].
• Furthermore, the UBA domain of p62 was necessary for aggregate sequestration and cell survival [8].
• The effects of the polyglutamine repeat protein ataxin-1 on the UbL-UBA protein A1Up [9].

Chemical compound and disease context of UBAP1

• In this study, we show the presence of a substance associated with auxin activity, identified as indole-3-butyric acid (IBA), in Azospirillum brasilense UAP 154 growth medium [10].
• The organic matrix extracted from kidney stones contained a protein antigenically related to I alpha I. We conclude that UAP is a member of I alpha I family taking part in inhibiting calcium oxalate crystallization, and modulating the formation of stones in the urinary tract [11].
• BACKGROUND: The purpose of the study was to prospectively evaluate the protective effect of nicorandil during percutaneous coronary intervention (PCI) in patients with unstable angina (UAP) [12].

Biological context of UBAP1

• Isolation and characterization of a novel cDNA, UBAP1, derived from the tumor suppressor locus in human chromosome 9p21-22 [13].
• Materials and Methods: Blood was obtained from 94 unrelated French PDB patients and 100 controls for mutation screening of exons 7 and 8, encoding for the UBA domain of SQSTM1 [14].
• Three novel missense mutations of SQSTM1 were identified in familial PDB, all affecting the UBA domain [15].
• Ubiquitin-associated domain mutations of SQSTM1 in Paget's disease of bone: evidence for a founder effect in patients of British descent [16].
• Novel UBA domain mutations of SQSTM1 in Paget's disease of bone: genotype phenotype correlation, functional analysis, and structural consequences [15].

Anatomical context of UBAP1

• The direct sequencing analysis of the coding region of hUBAP1 following RT-PCR failed to reveal any mutations in a preliminary screen of NPC cell line HNE1 and primary nasopharyngeal carcinoma samples [13].
• In summary, exposure to Zn2+ ions causes PTEN degradation and loss of function, which is mediated by an ubiquitin-associated proteolytic process in the airway epithelium [17].
• A variety of ubiquitin-associated (or conjugated) proteins, including substrates and enzymes for the ubiquitin system, are present in eukaryotic cells [18].
• The previously identified BMSC-UbP protein derived from bone marrow stromal cells contains a ubiquitin-associated (UBA) domain at the C terminus that has been implicated in linking cellular processes and the ubiquitin system [19].
• An increase in the activity of soluble PSA in the prefrontal cortex of heroin abusers was observed (heroin addict group: 51,452+/-3892 UAP/mg protein versus control group: 42,003+/-2597 UAP/mg protein; P<0.05), while the activity of the other peptidases in both brain regions remained unaltered [20].

Associations of UBAP1 with chemical compounds

• Rad23 ubiquitin-associated domains (UBA) inhibit 26 S proteasome-catalyzed proteolysis by sequestering lysine 48-linked polyubiquitin chains [21].
• Uronic-acid-rich protein (UAP) is a new urinary macromolecule which strongly inhibits calcium oxalate crystal formation [22].
• These included heat shock proteins (119.m00119 and 279.m00091), cyst wall glycoprotein Jacob (33.m00261), and ubiquitin-associated proteins (16.m00343; 195.m00092) [23].
• In the present study human and rat AM were reacted with a range of environmental particles, including oil fly ash (OFA), diesel dust (DD), and ambient air particles (UAP) collected in four urban centers [24].
• LPS priming amplified TNF production by both rat and human AMs in response to UAP and CAPs but not inert TiO2 [25].

Other interactions of UBAP1

• We report the X-ray structure of the catalytic and ubiquitin-associated domains (UBA) of human MARK2 [26].
• The ubiquitin-associated domain of hPLIC-2 interacts with the proteasome [27].
• Solution structure of the ubiquitin-associated domain of human BMSC-UbP and its complex with ubiquitin [19].

Analytical, diagnostic and therapeutic context of UBAP1

• Here, we report the results of a quantitative study of the interaction of K48-linked polyubiquitin chains with UBA domains of the DNA repair/proteolysis protein HHR23A, using surface plasmon resonance and other approaches [28].
• UAP exhibited a strong calcium oxalate crystallization inhibitory activity [29].
• Failed deployment occurred with nine stents in SAP patients, v four in UAP patients (NS) [5].
• On Western blotting, bikunin antibody immunoreacted with UAP and both urinary and plasma bikunins [29].
• The diagnostic sensitivity of Trop T for the detection of minor myocardial damage in UAP patients was the same as for cTnT ELISA [30].

References