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Gene Review

YEF3  -  translation elongation factor eEF1B...

Saccharomyces cerevisiae S288c

Synonyms: EF-3, EF-3A, EFC1, Elongation factor 3A, Eukaryotic elongation factor 3, ...
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Disease relevance of YEF3


High impact information on YEF3

  • Based on these findings, we propose that GCN1 performs an EF3-related function in facilitating the activation of GCN2 by uncharged tRNA on translating ribosomes [4].
  • The level of TEF-1 and -2 mRNA varied little among the cell morphological types studied, whereas TEF-3 mRNA was present in twofold greater quantity in sporangiospores than in either germlings or yeast cells which had been induced to undergo morphogenesis to hyphae [5].
  • These results support the model that upon ATP hydrolysis, eEF3 interacts with eEF1A to help catalyze the delivery of aminoacyl-tRNA at the A-site of the ribosome [6].
  • The binding of eEF3 to eEF1A is enhanced by ADP, indicating the interaction is favored post-ATP hydrolysis but is not dependent on the eEF1A-bound nucleotide [6].
  • A temperature-sensitive P915L mutant in the eEF1A binding site of eEF3 has reduced ATPase activity and affinity for eEF1A [6].

Chemical compound and disease context of YEF3


Biological context of YEF3


Anatomical context of YEF3

  • RLI1 is an essential yeast protein closely related in sequence to two soluble members of the ATP-binding cassette family of proteins that interact with ribosomes and function in translation elongation (YEF3) or translational control (GCN20) [11].
  • Evidence was obtained which indicated that EF-3 was essential for the translation of natural mRNA as well as poly(U), was associated with polysomes but not ribosomal subunits, and was required for every cycle in the elongation phase of protein synthesis [12].
  • The EF-3 purified from the cytosol by immunoaffinity chromatography was comparable to that prepared by ion-exchange chromatography [12].

Associations of YEF3 with chemical compounds

  • We cloned the coding sequence of the Saccharomyces YEF3 gene in this vector and demonstrated an increase in YEF3 protein levels when cells were grown in the presence of the sugar sorbitol [13].
  • A combination of 40 S subunits from yeast and "60 S" from wheat germ showed the stimulatory effect of EF-3 in polyphenylalanine synthesis (Chakraburtty, K., and Kamath, A. (1988) Int. J. Biochem. 20, 581-590) [14].
  • The EF-3 effect is manifest in the presence of ATP, GTP, or ITP [14].
  • The carboxy-terminus of EF-3 contains blocks of lysine boxes essential for its functional interaction with yeast ribosomes [10].
  • Yeast strains containing elevated EF-3 protein levels are more sensitive to the aminoglycoside antibiotics hygromycin and paromomycin [8].

Other interactions of YEF3

  • From these results, we conclude that the HEF3 gene does not encode a functional homolog of YEF3 [15].
  • The stimulatory effect of EF-3 is, in many respects, distinct from that of EF-1 beta [14].
  • This conclusion supports the model that an eEF3-related activity of GCN1 influences occupancy of the ribosomal decoding site by uncharged tRNA in starved cells [16].
  • ELF1 (Elongation Like Factor) showed greatest homology with a Saccharomyces cerevisiae ORF (YPL226W), whose function is unknown, and lower homology with fungal elongation factor 3 (EF-3) genes [17].
  • Furthermore, EF-3 was also found to display amino acid similarity to myosin proteins whose cellular function is to provide the motive force of muscle [18].

Analytical, diagnostic and therapeutic context of YEF3


  1. The N terminus of eukaryotic translation elongation factor 3 interacts with 18 S rRNA and 80 S ribosomes. Gontarek, R.R., Li, H., Nurse, K., Prescott, C.D. J. Biol. Chem. (1998) [Pubmed]
  2. Sequence analysis of the translational elongation factor 3 from Saccharomyces cerevisiae. Qin, S.L., Xie, A.G., Bonato, M.C., McLaughlin, C.S. J. Biol. Chem. (1990) [Pubmed]
  3. Evolutionary divergence of an elongation factor 3 from Cryptococcus neoformans. Blakely, G., Hekman, J., Chakraburtty, K., Williamson, P.R. J. Bacteriol. (2001) [Pubmed]
  4. Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2alpha kinase GCN2. Marton, M.J., Vazquez de Aldana, C.R., Qiu, H., Chakraburtty, K., Hinnebusch, A.G. Mol. Cell. Biol. (1997) [Pubmed]
  5. Expression of three genes for elongation factor 1 alpha during morphogenesis of Mucor racemosus. Linz, J.E., Sypherd, P.S. Mol. Cell. Biol. (1987) [Pubmed]
  6. Domain and Nucleotide Dependence of the Interaction between Saccharomyces cerevisiae Translation Elongation Factors 3 and 1A. Anand, M., Balar, B., Ulloque, R., Gross, S.R., Kinzy, T.G. J. Biol. Chem. (2006) [Pubmed]
  7. Purification and crystallization of the yeast translation elongation factor eEF3. Andersen, C.F., Anand, M., Boesen, T., Van, L.B., Kinzy, T.G., Andersen, G.R. Acta Crystallogr. D Biol. Crystallogr. (2004) [Pubmed]
  8. Protein synthesis in yeast. Structural and functional analysis of the gene encoding elongation factor 3. Sandbaken, M.G., Lupisella, J.A., DiDomenico, B., Chakraburtty, K. J. Biol. Chem. (1990) [Pubmed]
  9. Regulation by tetracycline of gene expression in Saccharomyces cerevisiae. Nagahashi, S., Nakayama, H., Hamada, K., Yang, H., Arisawa, M., Kitada, K. Mol. Gen. Genet. (1997) [Pubmed]
  10. Yeast elongation factor 3: structure and function. Chakraburtty, K., Triana-Alonso, F.J. Biol. Chem. (1998) [Pubmed]
  11. The essential ATP-binding cassette protein RLI1 functions in translation by promoting preinitiation complex assembly. Dong, J., Lai, R., Nielsen, K., Fekete, C.A., Qiu, H., Hinnebusch, A.G. J. Biol. Chem. (2004) [Pubmed]
  12. Monoclonal antibody specific for yeast elongation factor 3. Hutchison, J.S., Feinberg, B., Rothwell, T.C., Moldave, K. Biochemistry (1984) [Pubmed]
  13. Construction of a sorbitol-based vector for expression of heterologous proteins in Saccharomyces cerevisiae. McGonigal, T., Bodelle, P., Schopp, C., Sarthy, A.V. Appl. Environ. Microbiol. (1998) [Pubmed]
  14. Role of yeast elongation factor 3 in the elongation cycle. Kamath, A., Chakraburtty, K. J. Biol. Chem. (1989) [Pubmed]
  15. A highly conserved intraspecies homolog of the Saccharomyces cerevisiae elongation factor-3 encoded by the HEF3 gene. Maurice, T.C., Mazzucco, C.E., Ramanathan, C.S., Ryan, B.M., Warr, G.A., Puziss, J.W. Yeast (1998) [Pubmed]
  16. Polyribosome binding by GCN1 is required for full activation of eukaryotic translation initiation factor 2{alpha} kinase GCN2 during amino acid starvation. Sattlegger, E., Hinnebusch, A.G. J. Biol. Chem. (2005) [Pubmed]
  17. Disruption studies of a Candida albicans gene, ELF1: a member of the ATP-binding cassette family. Sturtevant, J., Cihlar, R., Calderone, R. Microbiology (Reading, Engl.) (1998) [Pubmed]
  18. Translation elongation factor-3 (EF-3): an evolving eukaryotic ribosomal protein? Belfield, G.P., Ross-Smith, N.J., Tuite, M.F. J. Mol. Evol. (1995) [Pubmed]
  19. Isolation of the yeast gene encoding elongation factor 3 for protein synthesis. Qin, S.L., Moldave, K., McLaughlin, C.S. J. Biol. Chem. (1987) [Pubmed]
  20. Elongation factor 3 (EF-3) from Candida albicans shows both structural and functional similarity to EF-3 from Saccharomyces cerevisiae. Colthurst, D.R., Schauder, B.S., Hayes, M.V., Tuite, M.F. Mol. Microbiol. (1992) [Pubmed]
  21. No detection of characteristic fungal protein elongation factor EF-3 in Pneumocystis carinii. Jackson, H.C., Colthurst, D., Hancock, V., Marriott, M.S., Tuite, M.F. J. Infect. Dis. (1991) [Pubmed]
  22. Domain structure analysis of elongation factor-3 from Saccharomyces cerevisiae by limited proteolysis and differential scanning calorimetry. Ladror, U.S., Egan, D.A., Snyder, S.W., Capobianco, J.O., Goldman, R.C., Dorwin, S.A., Johnson, R.W., Edalji, R., Sarthy, A.V., McGonigal, T., Walter, K.A., Holzman, T.F. Protein Sci. (1998) [Pubmed]
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