The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

pgi  -  glucose-6-phosphate isomerase

Escherichia coli O157:H7 str. Sakai

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ECs5008


High impact information on ECs5008

  • The energy-independent transhydrogenase UdhA, in contrast, was essential for growth under metabolic conditions with excess NADPH formation, i.e. growth on acetate or in a phosphoglucose isomerase mutant that catabolized glucose through the pentose phosphate pathway [6].
  • Because the PEP-glyoxylate cycle was also active in glucose excess batch cultures of an NADPH-overproducing phosphoglucose isomerase mutant, one function of this new central pathway may be the decoupling of catabolism from NADPH formation that would otherwise occur in the tricarboxylic acid cycle [7].
  • The deduced amino acid sequence of this first archaeal PGI revealed that it is not related to its bacterial and eukaryal counterparts [8].
  • We here report the isolation of the missing link of the pyrococcal glycolysis, the phosphoglucose isomerase (PGI), which was purified to homogeneity from P. furiosus and biochemically characterized [8].
  • The P. furiosus PGI, a dimer of identical 23.5-kDa subunits, catalyzes the reversible isomerization of glucose 6-phosphate to fructose 6-phosphate, with K(m) values of 1.99 and 0.63 mm, respectively [8].

Chemical compound and disease context of ECs5008


Biological context of ECs5008

  • However, excluding the alternative hypothesis of ancient gene duplication has proven difficult because of both insufficient sampling of taxa and an earlier misidentification of a bacterial Pgi sequence [2].
  • Molecular analysis of the plant gene encoding cytosolic phosphoglucose isomerase [13].
  • Mutagenesis of endopolygalacturonase from Fusarium moniliforme: histidine residue 234 is critical for enzymatic and macerating activities and not for binding to polygalacturonase-inhibiting protein (PGIP) [14].
  • We cloned the PGI gene from a genomic library prepared from a single plant of known PGI genotype [13].
  • No physiological role could be found for the existence of the two forms. -- Formation of phosphoglucose isomerase is under respiratory control: under anaerobiosis the enzyme (both species) is depressed parallely with other glycolytic enzymes [15].

Anatomical context of ECs5008


Associations of ECs5008 with chemical compounds

  • Kinetic analysis revealed that, unlike all known PGIs, the enzyme catalyzed reversible isomerization not only of glucose 6-phosphate but also of epimeric mannose 6-phosphate at similar catalytic efficiency, thus defining the protein as bifunctional phosphoglucose/phosphomannose isomerase (PGI/PMI) [17].
  • 2) A series of isogenic strains were made in which in vivo use of thereaction might differ, e.g. a wild type strain versus a mutant lacking 6-phosphogluconate dehydrase, as grown on gluconate; a phosphoglucose isomerase mutant grown on glucose or glycerol [18].
  • The transamidase mechanism of GPI anchoring was studied in bloodstream forms of Trypanosoma brucei using media containing hydrazine or biotinylated hydrazine [19].
  • Six electromorphs were found for PE2, G6P, and PGI, five for MDH, four for 6PG, and three for AK [20].
  • PE2, G6P, and PGI contributed most of the ET resolution (48 of 49 ETs) [20].

Analytical, diagnostic and therapeutic context of ECs5008


  1. Elevated glucose 6-phosphate levels are associated with plasmid mutations in vivo. Lee, A.T., Cerami, A. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  2. Transkingdom transfer of the phosphoglucose isomerase gene. Katz, L.A. J. Mol. Evol. (1996) [Pubmed]
  3. Cloning, characterization, and nucleotide sequence analysis of a Zymomonas mobilis phosphoglucose isomerase gene that is subject to carbon source-dependent regulation. Hesman, T.L., Barnell, W.O., Conway, T. J. Bacteriol. (1991) [Pubmed]
  4. Expression, purification, and crystallization of two isozymes of 6-phosphoglucose isomerase of Bacillus stearothermophilus. Hsiao, C.D., Chou, C.C., Hsiao, Y.Y., Sun, Y.J., Meng, M. J. Struct. Biol. (1997) [Pubmed]
  5. Biochemical characterization of recombinant phosphoglucose isomerase of Mycobacterium tuberculosis. Mathur, D., Ahsan, Z., Tiwari, M., Garg, L.C. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
  6. The soluble and membrane-bound transhydrogenases UdhA and PntAB have divergent functions in NADPH metabolism of Escherichia coli. Sauer, U., Canonaco, F., Heri, S., Perrenoud, A., Fischer, E. J. Biol. Chem. (2004) [Pubmed]
  7. A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli. Fischer, E., Sauer, U. J. Biol. Chem. (2003) [Pubmed]
  8. The phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus is a unique glycolytic enzyme that belongs to the cupin superfamily. Verhees, C.H., Huynen, M.A., Ward, D.E., Schiltz, E., de Vos, W.M., van der Oost, J. J. Biol. Chem. (2001) [Pubmed]
  9. Functional effects of PGI allozymes in Escherichia coli. Dykhuizen, D.E., Hartl, D.L. Genetics (1983) [Pubmed]
  10. New phosphoglucose isomerase mutants of Escherichia coli. Vinopal, R.T., Hillman, J.D., Schulman, H., Reznikoff, W.S., Fraenkel, D.G. J. Bacteriol. (1975) [Pubmed]
  11. Responses of the central metabolism in Escherichia coli to phosphoglucose isomerase and glucose-6-phosphate dehydrogenase knockouts. Hua, Q., Yang, C., Baba, T., Mori, H., Shimizu, K. J. Bacteriol. (2003) [Pubmed]
  12. Gene expression patterns for metabolic pathway in pgi knockout Escherichia coli with and without phb genes based on RT-PCR. Kabir, M.M., Shimizu, K. J. Biotechnol. (2003) [Pubmed]
  13. Molecular analysis of the plant gene encoding cytosolic phosphoglucose isomerase. Thomas, B.R., Laudencia-Chingcuanco, D., Gottlieb, L.D. Plant Mol. Biol. (1992) [Pubmed]
  14. Mutagenesis of endopolygalacturonase from Fusarium moniliforme: histidine residue 234 is critical for enzymatic and macerating activities and not for binding to polygalacturonase-inhibiting protein (PGIP). Caprari, C., Mattei, B., Basile, M.L., Salvi, G., Crescenzi, V., De Lorenzo, G., Cervone, F. Mol. Plant Microbe Interact. (1996) [Pubmed]
  15. Phosphoglucose isomerase from Escherischia coli K 10: purification, properties and formation under aerobic and anaerobic condition. Schreyer, R., Böck, A. Arch. Microbiol. (1980) [Pubmed]
  16. Characterization of cDNA encoding for phosphoglucose isomerase of rice (Oryza sativa L.). Nozue, F., Umeda, M., Nagamura, Y., Minobe, Y., Uchimiya, H. DNA Seq. (1996) [Pubmed]
  17. Bifunctional phosphoglucose/phosphomannose isomerases from the Archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily. Hansen, T., Wendorff, D., Schönheit, P. J. Biol. Chem. (2004) [Pubmed]
  18. The 6-phosphogluconate dehydrogenase reaction in Escherichia coli. de Silva, A.O., Fraenkel, D.G. J. Biol. Chem. (1979) [Pubmed]
  19. GPI anchor transamidase of Trypanosoma brucei: in vitro assay of the recombinant protein and VSG anchor exchange. Kang, X., Szallies, A., Rawer, M., Echner, H., Duszenko, M. J. Cell. Sci. (2002) [Pubmed]
  20. Application of multilocus enzyme gel electrophoresis to Haemophilus influenzae. Porras, O., Caugant, D.A., Lagergård, T., Svanborg-Edén, C. Infect. Immun. (1986) [Pubmed]
  21. Identification and characterization of SRS1, a Toxoplasma gondii surface antigen upstream of and related to SAG1. Hehl, A., Krieger, T., Boothroyd, J.C. Mol. Biochem. Parasitol. (1997) [Pubmed]
  22. Purification, crystallization and preliminary crystallographic analysis of phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus. Akerboom, J., Turnbull, A.P., Hargreaves, D., Fisher, M., de Geus, D., Sedelnikova, S.E., Berrisford, J.M., Baker, P.J., Verhees, C.H., van der Oost, J., Rice, D.W. Acta Crystallogr. D Biol. Crystallogr. (2003) [Pubmed]
  23. Prostacyclin and thromboxane A2 in septic shock: species differences. Yellin, S.A., Nguyen, D., Quinn, J.V., Burchard, K.W., Crowley, J.P., Slotman, G.J. Circ. Shock (1986) [Pubmed]
WikiGenes - Universities