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Gene Review

argR  -  l-arginine-responsive arginine metabolism...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3226, JW3206, Rarg, xerA
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Disease relevance of argR


High impact information on argR

  • This plasmid stabilizing recombination system requires the presence in cis of the ColE1 cer region, plus at least two trans-acting factors encoded by the xerA and xerB genes of Escherichia coli [6].
  • The argR protein binds to cer DNA both in vivo and in vitro in the presence of arginine [2].
  • Sequences within DsrA complementary to three additional genes, argR, ilvIH, and rbsD, suggest that DsrA is a riboregulator of gene expression that acts coordinately via RNA:RNA interactions at multiple loci [7].
  • We describe the nucleotide sequence of the argR gene, including its control region, and show that formation of the repressor is autoregulated [1].
  • The argR control region contains two promoters, one of which overlaps the operator site and, as with other arg genes, consists of two adjacent palindromic sequences ("ARG boxes") [1].

Chemical compound and disease context of argR

  • Ornithine transcarbamoylase was purified by affinity chromatography from Escherichia coli W argR- by using N-delta-(phosphonoacetyl)ornithine as the ligand [8].
  • A single free Cys sidechain in the N-terminal domain of the E. coli arginine repressor was covalently derivatized with S-cysteaminyl-EDTA for site-specific attachment of paramagnetic metal ions [9].
  • The filter element is demonstrated with a (1)H NOESY spectrum of a 28.5-kDa 2:1 complex between the uniformly (13)C-labeled N-terminal domain of Escherichia coli arginine repressor and operator DNA [10].
  • A plasmid carrying the arginine repressor gene (argR) of Escherichia coli was obtained out of the Clarke and Carbon colony bank which contains clones of individual ColE1 plasmids carrying fragments of the E. coli chromosome [11].

Biological context of argR


Associations of argR with chemical compounds

  • These effects appear to depend on the concurrent expression of the regulatory-gene and streptomycin resistance mutations, as indicated by analogous experiments with canavanine-resistant mutants of 250-10 that have partial argR- character [17].
  • This observation, together with our previous report that argR mutations impair the cumulative repression of CPSase, suggests that this control is mediated through the concerted effects of regulatory elements specific for the arginine and pyrimidine pathways [18].
  • Other mutants harbored wild-type argR genes, and 20 mutants have lost their ability to grow in normal air without carbon dioxide enrichment; this revealed a link between arginine biosynthesis and a still-unknown CO2-dependent metabolic pathway [19].
  • We demonstrate that this unusual behaviour for an ArgR protein can, to a large extent, be ascribed to the presence of a serine residue at position 107 of ArgR(Tn), instead of the highly conserved glutamine that is involved in arginine binding in the E.coli repressor [4].
  • Dissecting the molecular details of prokaryotic transcriptional control by surface plasmon resonance: the methionine and arginine repressor proteins [20].

Other interactions of argR

  • The three arginine biosynthetic operons argCJDBF, argGH, and gltS-argE were shown to be repressed by the products of argR and ahrC [14].
  • Using strains carrying defined argR, and rpoS mutations, we evaluated the relative contributions of these two regulators to the expression of argH using operon-lacZ fusions [21].

Analytical, diagnostic and therapeutic context of argR

  • Moreover, sequence analysis of 85 strains shows no evidence of selection at the arginine repressor locus [22].


  1. Nucleotide sequence of the argR gene of Escherichia coli K-12 and isolation of its product, the arginine repressor. Lim, D.B., Oppenheim, J.D., Eckhardt, T., Maas, W.K. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  2. The arginine repressor is essential for plasmid-stabilizing site-specific recombination at the ColE1 cer locus. Stirling, C.J., Szatmari, G., Stewart, G., Smith, M.C., Sherratt, D.J. EMBO J. (1988) [Pubmed]
  3. The highly thermostable arginine repressor of Bacillus stearothermophilus: gene cloning and repressor-operator interactions. Dion, M., Charlier, D., Wang, H., Gigot, D., Savchenko, A., Hallet, J.N., Glansdorff, N., Sakanyan, V. Mol. Microbiol. (1997) [Pubmed]
  4. Hyperthermophilic Thermotoga arginine repressor binding to full-length cognate and heterologous arginine operators and to half-site targets. Morin, A., Huysveld, N., Braun, F., Dimova, D., Sakanyan, V., Charlier, D. J. Mol. Biol. (2003) [Pubmed]
  5. Control of arg gene expression in Salmonella typhimurium by the arginine repressor from Escherichia coli K-12. Gardner, M.M., Hennig, D.O., Kelln, R.A. Mol. Gen. Genet. (1983) [Pubmed]
  6. xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase. Stirling, C.J., Colloms, S.D., Collins, J.F., Szatmari, G., Sherratt, D.J. EMBO J. (1989) [Pubmed]
  7. Riboregulation in Escherichia coli: DsrA RNA acts by RNA:RNA interactions at multiple loci. Lease, R.A., Cusick, M.E., Belfort, M. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  8. The inhibition of ornithine transcarbamoylase from Escherichia coli W by phaseolotoxin. Templeton, M.D., Sullivan, P.A., Shepherd, M.G. Biochem. J. (1984) [Pubmed]
  9. Site-specific labelling with a metal chelator for protein-structure refinement. Pintacuda, G., Moshref, A., Leonchiks, A., Sharipo, A., Otting, G. J. Biomol. NMR (2004) [Pubmed]
  10. Time-shared X(omega(1))-half-filter for improved sensitivity in subspectral editing. Andersson, P., Otting, G. J. Magn. Reson. (2000) [Pubmed]
  11. Isolation of plasmids carrying the arginine repressor gene argR of Escherichia coli K12. Eckhardt, T. Mol. Gen. Genet. (1980) [Pubmed]
  12. Binding of the arginine repressor of Escherichia coli K12 to its operator sites. Tian, G., Lim, D., Carey, J., Maas, W.K. J. Mol. Biol. (1992) [Pubmed]
  13. Mutational analysis of the arginine repressor of Escherichia coli. Tian, G., Maas, W.K. Mol. Microbiol. (1994) [Pubmed]
  14. ArgR and AhrC are both required for regulation of arginine metabolism in Lactococcus lactis. Larsen, R., Buist, G., Kuipers, O.P., Kok, J. J. Bacteriol. (2004) [Pubmed]
  15. Enhanced production of arginine and urea by genetically engineered Escherichia coli K-12 strains. Tuchman, M., Rajagopal, B.S., McCann, M.T., Malamy, M.H. Appl. Environ. Microbiol. (1997) [Pubmed]
  16. Complete sequence of the Salmonella typhimurium gene encoding malate dehydrogenase. Lu, C.D., Abdelal, A.T. Gene (1993) [Pubmed]
  17. Evidence for translational repression of arginine biosynthetic enzymes in Escherichia coli: altered regulation in a streptomycin-resistant mutant. Vogel, R.H., Devine, E.A., Vogel, H.J. Mol. Gen. Genet. (1978) [Pubmed]
  18. Repression of Escherichia coli carbamoylphosphate synthase: relationships with enzyme synthesis in the arginine and pyrimidine pathways. Piérard, A., Glansdorff, N., Gigot, D., Crabeel, M., Halleux, P., Thiry, L. J. Bacteriol. (1976) [Pubmed]
  19. Two arginine repressors regulate arginine biosynthesis in Lactobacillus plantarum. Nicoloff, H., Arsène-Ploetze, F., Malandain, C., Kleerebezem, M., Bringel, F. J. Bacteriol. (2004) [Pubmed]
  20. Dissecting the molecular details of prokaryotic transcriptional control by surface plasmon resonance: the methionine and arginine repressor proteins. Stockley, P.G., Baron, A.J., Wild, C.M., Parsons, I.D., Miller, C.M., Holtham, C.A., Baumberg, S. Biosensors & bioelectronics. (1998) [Pubmed]
  21. Stationary phase expression of the arginine biosynthetic operon argCBH in Escherichia coli. Weerasinghe, J.P., Dong, T., Schertzberg, M.R., Kirchhof, M.G., Sun, Y., Schellhorn, H.E. BMC Microbiol. (2006) [Pubmed]
  22. The argRB of Escherichia coli is rare in isolates obtained from natural sources. Merlo, L.M., Sadowsky, M.J., Ferguson, J.A., Dean, A.M. Gene (2006) [Pubmed]
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