Disease relevance of Liver, Artificial

• Efficacy of a larger version of the hybrid artificial liver support system using a polyurethane foam/spheroid packed-bed module in a warm ischemic liver failure pig model for preclinical experiments [1].
• Polyurethane foam/spheroid culture system using human hepatoblastoma cell line (Hep G2) as a possible new hybrid artificial liver [2].
• It may be expected to improve the cellular functions of the hepatoma cell line by Cx32 gene transfection and serve to develop an efficacious bioartificial liver [3].
• Clinical use of a bioartificial liver in the treatment of acetaminophen-induced fulminant hepatic failure [4].
• The Molecular Adsorbent Recirculating System (MARS) represents an attractive artificial liver support system for the treatment of liver insufficiency [5].

High impact information on Liver, Artificial

• CYP3A4 inducible model for in vitro analysis of human drug metabolism using a bioartificial liver [6].
• Further advances in the MARS components will definitively state whether albumin dialysis may represent the future in the field of artificial liver devices [7].
• These results are of relevance in the use of these cell lines to investigate acetaminophen hepatotoxicity, and may be of importance in the choice of cells for use in bioartificial liver support systems [8].
• For clinical utilization of extracorporeal liver perfusion as an artificial liver assist device, we examined the possibility of long-term xenoperfusion of the pig liver by the continuous administration of prostaglandin E1 (PGE1) and insulin [9].
• The advisability of using Matrigel or other extracellular matrix proteins in a bioartificial liver loaded with pig hepatocytes is discussed [10].

Chemical compound and disease context of Liver, Artificial

• AIM: To observe the adsorbent effect of resin on endotoxin, cytokine, bilirubin in plasma of patients with hepatic failure and to determine the resin perfusion as an artificial liver support system in the treatment of hepatic failure [11].
• Development of a bioartificial liver with glutamine synthetase-transduced recombinant human hepatoblastoma cell line, HepG2 [12].

Biological context of Liver, Artificial

• We tested the hypothesis that hepatocyte growth factor would improve viable cell density and decrease biotransformation functions of liver cells in monolayer culture and in hepatocytes entrapped in collagen cylindrical gel "noodles" as found in the extracorporeal bioartificial liver [13].
• RESULTS: After treatment with bioartificial liver, blood ammonia, prothrombin time and total bilirubin showed significant decrease [14].

Anatomical context of Liver, Artificial

• Bilirubin conjugation by an artificial liver composed of cultured cells and synthetic capillaries [15].
• CONCLUSION: Our differentiation media, including FGF-4 and OSM, are effective to differentiate bone marrow cells into hepatocyte-like cells, which could be used for hepatocyte resources for bioartificial liver or hepatocyte transplantation [16].

Associations of Liver, Artificial with chemical compounds

• A new extracorporeal bioartificial liver using alginate-entrapped hepatocytes was developed and evaluated for its ability to correct the lack of bilirubin conjugation in the Gunn rat [17].
• We have reported the usefulness of a polyurethane foam packed-bed culture system of hepatocyte spheroids as a hybrid artificial liver support system (PUF-HALSS) [1].
• Fibrin membrane endowed with biological function. VII. An approach to an artificial liver; conversion of ammonia to urea in vitro [18].
• Crosslinked collagen/chitosan matrix for artificial livers [19].
• Deliverance. Medicine closes in on an artificial liver device [20].

Gene context of Liver, Artificial

• To develop a bioartificial liver with a human cell line, GS gene was transfected into HepG2 cells, which had no ammonia removal activity [21].
• With the aim of adding lost or deficient functions and producing cell lines for the bioreactor of a hybrid artificial liver, rat glutamine synthetase (GS) gene was transfected into Chinese hamster ovary (CHO) cells, because it is able to lower the ammonia level [22].
• Fourteen patients with coma received artificial liver support that we devised [23].
• Physico-chemical aspects of the removal of protein-bound substances by charcoal and other adsorbents of potential value in systems of artificial liver support: Part 2--kinetics of removal [24].
• Why bioartificial liver support remains the Holy Grail [25].

Analytical, diagnostic and therapeutic context of Liver, Artificial

• The maximum rate of glucose production by the hepatocyte bioartificial liver ranged from 50-80 micrograms/h. Plasma concentrations of aromatic amino acids, proline, alanine, and ammonia were normalized in anhepatic rabbits during hepatocyte hemoperfusion [26].
• Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver [27].
• The bioartificial liver, containing either microcarrier hepatocytes (n = 6) or microcarriers alone (n = 5); was attached to adult Gunn rats via cannulas in the aorta and inferior vena cava [28].
• To realize the high density culture of NKNT-3 cells in a bioartificial liver device, we have developed cellulose microspheres (CMS) which contain cell adhesive GRGDS (Gly-Arg-Gly-Asp-Ser) peptides [29].
• Immobilization of multienzymes of urea cycle into fibrin membrane: an approach to an artificial liver [30].

References