Disease relevance of False Positive Reactions

• Sera from all 61 patients with a variety of autoimmune and other diseases known to be associated with biologically false-positive reactions for syphilis were negative by this ELISA-SY [1].
• The inhibitor, Irgasan DP-300, was incorporated to eliminate false-positive reactions from most remaining Enterobacteriaceae [2].
• Most false-positive reactions resulted with Ehrlichia chaffeensis; when these sera were excluded, the specificity of most antigens was 91 to 100% [3].
• When 177 sera from patients with fungal diseases other than paracoccidioidomycosis were tested by immunodiffusion, there were no false-positive reactions [4].
• An immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (MACELISA) was developed for the detection of pseudorabies virus (PRV)-specific IgM antibody in swine sera because false-positive reactions frequently occurred when sera from older swine were tested with an indirect IgM enzyme-linked immunosorbent assay [5].

High impact information on False Positive Reactions

• The frequencies of false-positive reactions in a "tricky" panel of samples from patients with autoimmune and acute viral diseases and in a blood-donor panel were Abbott 9.5%, 0.42%: Organon 1.7%, 0%; Litton 1.0%, 0.4%; Behring 2.7%, 0.06%; Wellcome 0%, 0%; and Pasteur 0%, 0.02% [6].
• The Hemoccult methods had 65% and 50% false-positive reactions while the Hematest had 25% and 65% false-positive reactions after ferrous sulfate and ferrous gluconate, respectively [7].
• No false-positive reactions have been obtained with pooled or individual sera from patients infected with numerous parasites other than S. japonicum, although no information is yet available on the inhibitory activities of sera fro S. mansoni- or S. hematobium-infected individuals [8].
• Failure to detect type-C virus p30-related antigen in systemic lupus erythematosus: false-positive reaction due to protease activity [9].
• The Farr test with standard buffer was found to be unsatisfactory for the measurement of anti-nDNA antibodies in dog sera due to a high incidence of false positive reactions; these could be eliminated by the inclusion of sodium dodecyl sulfate in the buffer system [10].

Chemical compound and disease context of False Positive Reactions

• We also found that serum specimens from healthy individuals contained antibodies cross-reactive with HHV-8 glycoprotein B that can potentially cause false-positive reactions in lytic PEL-based IFAs [11].

Biological context of False Positive Reactions

• Phosphocholine (PC), an immunodominant molecule present on a wide range of organisms, including filariae, evokes antibody responses that lead to false-positive reactions in routine serological assays [12].
• Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections [13].

Anatomical context of False Positive Reactions

• All the positive sera were then absorbed with porcine red blood cells and retested to exclude the false positive reaction due to nonspecific serum components [14].
• Blood stained cerebrospinal fluid responsible for false positive reactions of latex particle agglutination tests [15].

Associations of False Positive Reactions with chemical compounds

• There was no increase in the rate of false-positive reactions over the range of 2 to 5 cm distance from the sites of positive reactions to allergen or histamine [16].
• Causes, consequences, and recognition of false-positive reactions for ketones [17].
• The reliability of latex coagglutination testing for the serological grouping of hemolytic streptococci is limited by the relatively high incidence of false-positive reactions [18].
• A false-positive reaction was observed in six samples (33.3%) in the oxybuprocaine group [19].
• Although the isopropanol precipitation test is a commonly used and sensitive test for detecting unstable hemoglobins, false-positive reactions are frequently observed [20].

Gene context of False Positive Reactions

• False positive reaction for carcinoembryonic antigen in Paget cells. Immunohistochemical observation [21].
• The use of F(ab')2 antibody fragments to coat plate wells and sample diluent containing 5% NMS has resulted in an EIA for bovine IFN-gamma that is virtually free from false-positive reactions, has a high degree of reproducibility and a sample detection limit equivalent to approximately 80 pg/ml recombinant bovine IFN-gamma [22].
• When CRP was determined by the latex agglutination method, RF caused both increased titers for CRP and false positive reactions for CRP [23].
• Pepsin-digested IgG retained anti-65 KD reactivity, indicating that false-positive reaction due to the presence of IgG Fc portion and/or RF was unlikely [24].
• During the development of our NT4/5 immunoassay, we discovered that false positive reactions resulted when tissues were extracted and assayed under certain conditions [25].

Analytical, diagnostic and therapeutic context of False Positive Reactions

• When serum samples were treated by the alkaline denaturation method, some false-positive reactions were apparent with the digoxigenin-labeled probe, although their frequency could be reduced to around 8% by modifying the sample treatment with a centrifugation step [26].
• None of these 51 specimens reacted with native gp46 and/or gp61/68 in a radioimmunoprecipitation assay, suggesting a false-positive reaction (9.75%) [27].
• A small proportion of apparently false-positive reactions were observed, in that some sera, although reactive with the monoclonal antibodies, were not positive in conventional immunoassays using polyclonal antisera, nor were they neutralisable with polyclonal anti-HBs [28].
• However, intradermal tests were essential to exclude false positive reactions and detect all relevant steroid allergies in any individual patient [29].
• Removal of false-positive reactions from plasma in an enzyme immunoassay for bovine interferon-gamma [22].

References