Disease relevance of Thioglycerine

• Inhibition of respiration of Escherichia coli by thioglycerol [1].

High impact information on Thioglycerine

• In addition, the simultaneous presence of both TGA-like ligand and 1-thioglycerol or 2-mercaptoethylamine leads to formation of colloidally stable 1D CdTe nanostructures with controlled aspect ratios [2].
• Water-soluble luminescent CdS quantum dots (QDs) capped by polyphosphate, L-cysteine, and thioglycerol were synthesized in aqueous solution [3].
• Preincubation of microsomes in the presence of monothioglycerol or DTT resulted in an increased reductase activity, suggesting a possible inactive enzyme precursor(s) consisting of disulfide-linked units [4].
• Depression of adenosylmethionine content of Escherichia coli by thioglycerol [5].
• Anaerobic growth on glucose significantly protected Escherichia coli from growth inhibition by thioglycerol [1].

Chemical compound and disease context of Thioglycerine

• Mu dX(lac) insertion mutants of Escherichia coli CSH50 in which the expression of the lacZ gene was sensitive to the presence of exogenous 1-thioglycerol or dithiothreitol were isolated [6].
• Here the levels of delta-aminolevulinate biosynthetic enzymes in untreated and TG-treated E. coli THU and PUC2 (a mutant of THU which overproduces porphyrins in the presence of thiols) cells were determined [7].

Biological context of Thioglycerine

• Methionine and anaerobiosis completely overcame growth inhibition by 2 to 90 mM thioglycerol [1].
• Complement activation by 3-mercapto-1,2-propanediol immobilized on gold surfaces [8].
• In addition to high sensitivity as compared to optical detection, the simultaneous detection of free thiols and disulfides can be used to study the kinetics of these conversions, as is shown for MTG [9].
• The influence of alpha thioglycerol on erythropoiesis in fetal mouse liver cell cultures [10].

Anatomical context of Thioglycerine

• The calf brain phospholipid N-methyltransferase activity has also been solubilized by treating the membranes ultrasonically in the presence of Triton X-100 and 10 mM monothioglycerol [11].
• Monolayers of glutathione (GSH) and 3-mercapto-1,2-propanediol (MG) on gold were tested for their bioreactivity by assessing the degree of inflammatory reaction as manifested by the adherence and activation of platelets and white blood cells (wbc) after exposure to blood ex vivo [12].
• 1-thioglycerol (1-TG) was investigated as a potential GK inhibitor in adrenal gland, an organ consistently affected, and in cultured fibroblasts, available from affected individuals [13].
• Enzyme activity is poorly expressed in the absence of thiol groups, the presence of monothioglycerol stimulating uterine and chorion enzymes by 5- and 15-fold, respectively [14].
• It was affected by monothioglycerol and its binding was found to be significantly increased on cytosol dilution, as well as by estrogen-treatment [15].

Associations of Thioglycerine with other chemical compounds

• We report the effect of serum pH and of the presence or absence of mercaptoethanol, N-acetyl-L-cysteine, monothioglycerol, ethylenediaminetetraacetate, and ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetate on the activation of the human creatine kinase isoenzymes [16].
• Tissue was homogenized in 10 mM Tes buffer (pH 7.4 at 25 degrees C) containing 0.25 M sucrose, 20 mM Na2MoO4, 12 mM monothioglycerol, and 1.5 mM EDTA [17].
• Time-monitoring experiments conducted with the acetate salts of DAVP and AVP in common matrices such as thioglycerol, dithiothreitol + diethioerythritol, glycerol, hydroxyethyldisulfide and nitrobenzyl-alcohol demonstrate an important effect of the chemical nature of the matrix on reduction [18].
• The presence of 2-10 mM dithiothreitol (DTT), beta-mercaptoethanol (beta-MER), and monothioglycerol (MTG) caused a 2-3 fold increase in the [3H]TA and [3H]RU486 binding to GR [19].
• Analysis of the same peptides in thioglycerol by positive or negative ion FAB yielded peak intensity distributions for the protonated molecule which matched those predicted from the natural abundance of stable isotopes [20].

Gene context of Thioglycerine

• 1-Thioglycerol: inhibitor of glycerol kinase activity in vitro and in situ [13].
• Thioglycerol, 20 mmol/liter, is superior for both in-assay reactivation and for storage stability of sera [21].
• TG treatment elevated the activity of glutamyl-tRNA reductase in both strains [7].
• These findings suggest that thioglycerol should be the FAB matrix in analyses of peptides when determination of elemental or isotopic composition is desired [20].
• First, tyrosinase oxidation of [3H]-estradiol was markedly inhibited by sulfhydryl reducing agents (monothioglycerol) that stabilize [3H]-estradiol binding to estrogen receptor [22].

Analytical, diagnostic and therapeutic context of Thioglycerine

• Pulse-labeled proteins from aerobically growing, 1-thioglycerol-treated E. coli were separated by two-dimensional gel electrophoresis, and their radioactivities were compared with those of identical proteins from nontreated cells [23].
• The native gangliosides GM3, GM1, Fuc-GM1, GD1a, GD1b, Fuc-GD1b, GT1b and GQ1b were analysed by fast atom bombardment mass spectrometry (FAB-MS) in the negative ion mode in a matrix of thioglycerol [24].
• A 39 kD protein has been extracted from barley flour with 0.1 M monothioglycerol at pH 5.0 and purified by (NH4)2SO4-precipitation, anion exchange and molecular sieve chromatography [25].

References