Disease relevance of o-Tolidine

• The number of revertants was increased in the presence of S9 mix by o-tolidine in the Salmonella typhimurium strains TA98 and TA1538, and by TDDM in strains TA98 and TA100 [1].
• It is of importance to early recognize any acutely ill patient as having rhabdomyolysis syndrome when there is elevation of muscle enzymes, hypocalcemic seizure, positive orthotolidine reaction of urine strip while negative finding of red blood cell [2].

High impact information on o-Tolidine

• Bone marrow preparations stained with CD44 antibodies and visualized with 125I antimouse Ig (F(ab')2) followed by autoradiography showed positive staining of lymphocytes and myeloid cells but not of most orthotolidine-positive erythroblasts [3].
• We compared the orthotolidine method for quantifying haemoglobin in gastric juice with a method based on measurement of fluorescent porphyrins extracted from haem [4].
• Results of the orthotolidine test were not positively correlated with those of erythrocyte labeling, indicating that peroxidase tests should not be relied upon to detect blood in gastric aspirates [5].
• Identification by thin-layer chromatography of dyestuffs based on benzidine, o-tolidine or o-dianisidine in consumer fabric dye products [6].
• Evans Blue, another o-tolidine-based dye, elicited a greater DNA-repair response in hamster hepatocytes [7].

Chemical compound and disease context of o-Tolidine

• This paper confirms the mutagenicity of 2 carcinogenic chemicals, o-tolidine and 4,4'-tetramethyldiaminodiphenylmethane (TDDM). o-Tolidine and TDDM were both tested in the Salmonella/mammalian-microsome test and in the sister-chromatid exchange (SCE) test with rabbit lymphocytes in vitro [1].
• When hydrochloride salts of the parent compounds were made to improve their stability for animal feeding experiments, the mutagenicity for the Salmonella tester strains was reduced except for 3,3'-dimethylbenzidine [8].

Biological context of o-Tolidine

• In the neutralization test viral replication was demonstrated by the hemadsorption of guinea pig erythrocytes, the visibility of which could be further enhanced by the use of o-tolidine [9].
• However, stains exposed to benzidine dissolved in glacial acetic acid, leucomalachite green, and o-tolidine failed to yield high-molecular-weight DNA or to produce any RFLP patterns [10].

Anatomical context of o-Tolidine

• In tissue exposed to sucrose before or after being reacted with o-tolidine, the HRP reaction product is less electron dense and is found only in lysosomes outside the nucleus or occasionally in proximal cellular processes [11].
• Transverse cryostat sections of skeletal muscle were fixed in a solution containing 1.5% glutaraldehyde and 1.5% sulfosalicylic acid and stained in a solution containing equal volumes of 3% hydrogen peroxide and 50% ethanol saturated with o-tolidine [12].

Associations of o-Tolidine with other chemical compounds

• The TMB, BDHC, and o-tolidine reaction products were all found to be suitable for electron microscopy [13].
• Autoradiographic analyses demonstrated the binding of benzidine and 3,3'-dimethylbenzidine to both alpha 1- and beta-lipoproteins [14].
• The purpose of this work was to conduct a comparative study of the sensitivity and specificity of phenolphthalein, tetramethylbenzidine, leucomalachite green, and orthotolidine as presumptive tests for blood [15].

Gene context of o-Tolidine

• False negative tests for urine myoglobin by o-tolidine reaction after (NH4)2SO4 extraction occurred in four cases [16].
• A review of the records of 133 patients admitted with onset of IDDM disclosed one additional patient with marked myoglobinuria, and 11 patients with orthotolidine-positive urine in the absence of hematuria [17].
• The kinetic characteristics (k(cat) and K(m)) of benzidine (BD), 3,3'-dimethylbenzidine (o-tolidine), 3,3'-dimethoxybenzidine (o-dianisidine), and TMB oxidation at 25 degrees C in 0.05 M phosphate-citrate buffer, pH 5.5, catalyzed by hTPO and horseradish peroxidase (HPR) were determined [18].

Analytical, diagnostic and therapeutic context of o-Tolidine

• The o-tolidine is mutagenic in the Ames Salmonella/mammalian-microsome mutagenicity test when activated by a rat liver S9 preparation [19].
• Eight different diets were each fed to 6 cats to evaluate the effect on a guaiac and an o-tolidine fecal occult blood test [20].
• Guaiac reagent and orthotolidine (Hematest) tablets were extremely sensitive, but yielded false-positive reaction rates of 72% and 76%, respectively on the 240 stool specimens compared [21].
• MATERIALS AND METHODS: Twenty-nine kinds of detergents were added to a Hb solution and measured Hb concentration by a quantitative chemical occult blood test (modified colorimetric o tolidine method) and the results were compared with those by the immunological test [22].

References