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Gene Review

H2-Q7  -  histocompatibility 2, Q region locus 7

Mus musculus

Synonyms: H-2 class I histocompatibility antigen, Q7 alpha chain, H-2Q7, Ped, Q9, QA-2 antigen, ...
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Disease relevance of H2-Q7

  • Correction of defects responsible for impaired Qa-2 class Ib MHC expression on melanoma cells protects mice from tumor growth [1].
  • Q9-mediated protective immunity is lost or greatly diminished in mice deficient in CTL, including beta(2)-microglobulin knockout (KO), CD8 KO, and SCID mice [2].
  • Similar analysis of thymoma cells transfected with cloned C57BL/10 genes showed that cell-surface Qa-2 molecules encoded by a Q7b cDNA and the Q7b or Q9b gene were sensitive to hydrolysis by PtdIns-PLC, whereas the H-2Kb and H-2Db gene products were resistant [3].
  • In this study, we report that surface-expressed Q9 on 3LLA9F1 Lewis lung carcinoma and RMA T cell lymphoma also induces potent antitumor CTL responses [4].

High impact information on H2-Q7

  • These results revealed that the Qa-2k antigen was distinct from the normal Qa-2 antigen expressed on H-2b lymphocytes although it cross-reacted with some Qa-2-specific mAbs [5].
  • Our results suggest that the tissue-specific expression of Qa-2 may be controlled, in part, by mechanisms of alternate RNA splicing [6].
  • In cultured cells the alternative splicing of the Qa-2 message is induced by T-cell activation splicing of the Qa-2 message is induced by T-cell activation with concanavalin A [7].
  • While the H-2 antigens span the cell membrane, the Qa-2 molecules are attached to the cell surface via phospholipid anchors [7].
  • In some cell lines almost all Qa-2 transcripts lack exon 5 [7].

Biological context of H2-Q7


Anatomical context of H2-Q7

  • It was found that all detectable Qa-2 antigen on the embryonic cell surface is sensitive to cleavage by PI-PLC and is therefore bound to the cell membrane by a GPI linkage [12].
  • It was found that all four genes are transcribed in lymphocytes, but only Q7 and Q9 are transcribed in mouse embryos [10].
  • Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen [7].
  • The canonical mRNA encoding the membrane form of Qa-2 predominates in unstimulated mouse tissues but the cultured cell lines, like activated T cells, express enhanced levels of the truncated mRNA [7].
  • Both NK cells and CTLs appear to collaborate in restraining growth of Q9-positive tumors [1].

Associations of H2-Q7 with chemical compounds

  • Furthermore, treatment of Qa-2 proteins from both sources with cyanogen bromide or alpha-chymotrypsin resulted in identical peptide fragmentation patterns [13].
  • These results therefore provide a biochemical correlation between a cloned Qa-region gene produce expressed on the surface of transfected cells, and the Qa-2 glycoprotein on spleen cells that was described a decade ago by serologic methods [13].
  • Susceptibility or resistance in BALB/c substrains may be associated to differences known to distinguish them, such as serum testosterone levels and Qa-2 protein expression [14].
  • As a result, Qa-2 proteins cluster in cholesterol- and sphingolipid-rich lipid raft microdomains in the cell membrane and can signal via raft-associated intracellular signaling molecules [15].
  • L1210 expresses classical H-2 class I molecules, and since it has been shown that DTIC treatment does not modify the expression of these molecules, this is a suitable model to study nonclassical class I antigens, such as Qa2 glycoproteins, and their potential role in tumorigenicity [16].

Regulatory relationships of H2-Q7


Other interactions of H2-Q7

  • Thus, both Q7 and Q9 are candidates for the genes responsible for the Ped gene phenotype [10].
  • We found that the microinjected individual Q7 and Q9 genes increased the rate of preimplantation development [9].
  • Among the best characterized non-classical mouse major histocompatibility antigens are the Qa-2 molecules [7].
  • Northern (RNA) blot hybridizations, polymerase chain reaction studies, and cDNA cloning experiments demonstrate that EC lines transcribe genes allelic to the Tla region gene "37", Qa-2 region gene "Q7", and another, previously uncharacterized, class I-like gene [18].
  • Qa-region class I gene expression: identification of a second class I gene, Q9, encoding a Qa-2 polypeptide [19].

Analytical, diagnostic and therapeutic context of H2-Q7

  • Microinjection of the Q7 and/or Q9 genes resulted in protein expression in 10-25% of the microinjected embryos [9].
  • To confirm this apparent lack of genetic polymorphism, the polymerase chain reaction (PCR) technique was used to amplify a portion of the 3' end of the Q region genes, Q4 to Q9, from several independent wild-derived strains of mice [11].
  • Therefore, in this study, the sensitivity of cell-surface Qa-2, H-2Kb, and H-2Db to hydrolysis by PtdIns-PLC was investigated biochemically by immunoprecipitation of radioiodinated molecules from cell lysates or supernatants [3].
  • After treatment with endoglycosidase F, the Qa-2 polypeptide chains derived from C57BL/10 spleen and Q7b-transfected R1.1 cells displayed identical mobilities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis because of removal of N-linked oligosaccharide residues [13].
  • Using an enzyme-linked immunosorbent assay with an IgD-specific mAb and Qa-2-lacZ fusion protein, the existence of IgD in RS with specificity for Qa-2 was confirmed [20].


  1. Correction of defects responsible for impaired Qa-2 class Ib MHC expression on melanoma cells protects mice from tumor growth. Chiang, E.Y., Henson, M., Stroynowski, I. J. Immunol. (2003) [Pubmed]
  2. A nonclassical MHC class I molecule restricts CTL-mediated rejection of a syngeneic melanoma tumor. Chiang, E.Y., Stroynowski, I. J. Immunol. (2004) [Pubmed]
  3. Molecular mapping of signals in the Qa-2 antigen required for attachment of the phosphatidylinositol membrane anchor. Waneck, G.L., Sherman, D.H., Kincade, P.W., Low, M.G., Flavell, R.A. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  4. Protective immunity against disparate tumors is mediated by a nonpolymorphic MHC class I molecule. Chiang, E.Y., Stroynowski, I. J. Immunol. (2005) [Pubmed]
  5. Expression of the Qa-2k phenotype encoded by the Q5k gene on the surface of tumor cells derived from H-2k mice. Seo, N., Okazaki, T., Nakanishi-Ito, C., Tanino, T., Matsudaira, Y., Takahashi, T., Egawa, K. J. Exp. Med. (1992) [Pubmed]
  6. Tissue-specific expression of cell-surface Qa-2 antigen from a transfected Q7b gene of C57BL/10 mice. Waneck, G.L., Sherman, D.H., Calvin, S., Allen, H., Flavell, R.A. J. Exp. Med. (1987) [Pubmed]
  7. Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen. Ulker, N., Lewis, K.D., Hood, L.E., Stroynowski, I. EMBO J. (1990) [Pubmed]
  8. Modulation of preimplantation embryonic development by antisense oligonucleotides to major histocompatibility complex genes. Xu, Y., Jin, P., Warner, C.M. Biol. Reprod. (1993) [Pubmed]
  9. Identification of two major histocompatibility complex class Ib genes, Q7 and Q9, as the Ped gene in the mouse. Wu, L., Feng, H., Warner, C.M. Biol. Reprod. (1999) [Pubmed]
  10. Sequence and transcription of Qa-2-encoding genes in mouse lymphocytes and blastocysts. Cai, W., Cao, W., Wu, L., Exley, G.E., Waneck, G.L., Karger, B.L., Warner, C.M. Immunogenetics (1996) [Pubmed]
  11. Genetic polymorphisms of Q region genes from wild-derived mice: implications for Q region evolution. Tine, J.A., Walsh, A., Rathbun, D., Leonard, L., Wakeland, E.K., Dilwith, R., Flaherty, L. Immunogenetics (1990) [Pubmed]
  12. Removal of Qa-2 antigen alters the Ped gene phenotype of preimplantation mouse embryos. Tian, Z., Xu, Y., Warner, C.M. Biol. Reprod. (1992) [Pubmed]
  13. Qa-2 antigen encoded by Q7b is biochemically indistinguishable from Qa-2 expressed on the surface of C57BL/10 mouse spleen cells. Sherman, D.H., Waneck, G.L., Flavell, R.A. J. Immunol. (1988) [Pubmed]
  14. Genetic control of susceptibility to Taenia crassiceps cysticercosis. Fragoso, G., Lamoyi, E., Mellor, A., Lomeli, C., Govezensky, T., Sciutto, E. Parasitology (1996) [Pubmed]
  15. HLA-G Is Found in Lipid Rafts and Can Act as a Signaling Molecule. Comiskey, M., Domino, K.E., Warner, C.M. Hum. Immunol. (2007) [Pubmed]
  16. Down regulation of Qa gene expression on drug-modified tumor cells. Leroy, E., Lattuada, D., Casnici, C., Franco, P., Marelli, O.E. Tumori. (1993) [Pubmed]
  17. Differential expression of Ped gene candidates in preimplantation mouse embryos. Wu, L., Exley, G.E., Warner, C.M. Biol. Reprod. (1998) [Pubmed]
  18. Embryonal carcinoma cells express Qa and Tla class I genes of the major histocompatibility complex. Ostrand-Rosenberg, S., Nickerson, D.A., Clements, V.K., Garcia, E.P., Lamouse-Smith, E., Hood, L., Stroynowski, I. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  19. Qa-region class I gene expression: identification of a second class I gene, Q9, encoding a Qa-2 polypeptide. Soloski, M.J., Hood, L., Stroynowski, I. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  20. Detection of allogeneic Qa/TL and Ly specificities on murine tumor cells with IgD in tumor-regressor serum. Tanino, T., Seo, N., Okazaki, T., Nakanishi-Ito, C., Sekimata, M., Egawa, K. Cancer Immunol. Immunother. (1992) [Pubmed]
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