Disease relevance of Sfxn1

• These results suggest that hypothyroidism affects TCC expression at both the transcriptional and post-transcriptional levels [1].
• The effect of hyperthyroidism on the activity of the mitochondrial tricarboxylate carrier has been studied [2].
• Interstitial PDT with ALA/PpIX is equally effective in treating prostate cancer and TCC in these heterotopic rat models [3].
• Metabolism and toxicity of halogenated carbanilides: absorption, distribution and excretion of radioactivity from 3,4,4'-trichloro[14C]carbanilide (TCC) and 3-trifluoromethyl-4,4'-dichloro[14C]carbanilide (TFC) in rats [4].

High impact information on Sfxn1

• These sequences include a consensus element common to several neuronal genes, a TCC repeat, a cAMP response element, and two TATA boxes that were 134 nucleotides apart from each other [5].
• Hypothyroidism reduces tricarboxylate carrier activity and expression in rat liver mitochondria by reducing nuclear transcription rate and splicing efficiency [1].
• The tricarboxylate carrier (TCC), also known as citrate carrier, is an integral protein of the mitochondrial inner membrane [1].
• Hypothyroidism did not influence TCC mRNA stability [1].
• Furthermore, we found that the ratio of polyadenylated/unpolyadenylated TCC RNA as well as the length of the TCC RNA poly(A) tail were similar in both euthyroid and hypothyroid rats [1].

Biological context of Sfxn1

• The tricarboxylate (citrate) carrier (TCC), a protein of the mitochondrial inner membrane, is an obligatory component of the shuttle system by which mitochondrial acetyl-CoA is transported into the cytosol, where lipogenesis occurs [6].
• The aim of this study was to investigate the molecular basis for the regulation of TCC gene expression by a high-fat, n-6 PUFA-enriched diet [6].
• The properties of the reconstituted carrier, i.e., requirement for a counteranion, substrate specificity and inhibitor sensitivity, were similar to those of the tricarboxylate carrier purified from rat liver mitochondria [7].
• Wistar rats were subjected to orthodontic forces, in the absence or presence of treatment with a decoy molecule mimicking a nonsymmetric NF-kappaB binding site (5'-CGC TGG GGA CTT TCC ACG G-3') [8].
• Almost all areas of squamous cell proliferation in TCC were negative for cathepsin E [9].

Anatomical context of Sfxn1

• The tricarboxylate carrier has recently been purified from rat liver mitochondria by three distinct scientific groups using different methods [10].
• CTPL/Sfxn3 was targeted to mitochondrial membrane like Sfxn1 [11].
• The mitochondrial tricarboxylate carrier (TCC) plays an important role in lipogenesis being TCC-responsible for the efflux from the mitochondria to the cytosol of acetyl-CoA, the primer for fatty acid synthesis [12].
• At this time, the estimated half-life of TCC mRNA was the same in the hepatocytes from rats on both diets, whereas the transcriptional rate of TCC mRNA, tested by nuclear run-on assay, was reduced by approximately 38% in the rats on the SO-enriched diet [6].
• PURPOSE: Tumor spillage from bladder perforation during transurethral resection of a bladder tumor or during cystectomy risks seeding the peritoneum with TCC [13].

Associations of Sfxn1 with chemical compounds

• This effect of ATP was observed in the presence of oligomycin and could not be attributed to a change in the content of the known tricarboxylate carrier inhibitor, palmitoyl-CoA, nor to the ATP concentration [14].
• Inhibition of the mitochondrial tricarboxylate carrier by arginine-specific reagents [15].
• Purification of the active mitochondrial tricarboxylate carrier by hydroxylapatite chromatography [16].
• The mitochondrial tricarboxylate carrier has been extracted from rat liver mitochondria or SMP with Triton X-100, in the presence of 1,2,3-BTA and DPG, and partially purified by chromatography on HTP [16].
• The expression of the mitochondrial transporters, carnitine palmitoyltransferase 1 and the tricarboxylate carrier, is unaffected under these conditions [17].

Analytical, diagnostic and therapeutic context of Sfxn1

• Gel mobility shift assays demonstrate that WT1 binds to the TCC repeat sequences of the rat EGFR promoter [18].
• CONCLUSIONS: Intraperitoneal chemotherapy prevents TCC implantation in a murine model of tumor spillage [13].
• MATERIALS AND METHODS: Laparotomy was performed in 28 Fischer 344 rats (National Cancer Institute, Frederick, Maryland) to instill 1 x 10 AY-27 TCC cells [13].
• In separate experiments, after repeated oral administration of 3,4,4'-trichlorocarbanilide (TCC) and 3-trifluoromethyl-4,4'-dichlorocarbanilide (TFC) to rats, the biliary metabolites of each were isolated and identified [19].
• Detection of the TCC moiety after HPLC separation is by UV absorption at 265 nm; quantitation by peak height measurement [20].

References