High impact information on Acph-1

• In this paper we describe changes in microtubule and actin filament network compared to the progress of DNA fragmentation and redistribution of acid phosphatase [1].
• 5. Acid phosphatase-1 is inhibited by L(+)-tartrate (but not D(-)-tartrate), phosphate, and fluoride [2].
• Morphological alterations in the Golgi complex (GC) and changes in the distribution of acid phosphatase (AcPase), thiamine pyrophosphatase (TPPase), complex carbohydrates and reduced osmium tetroxide compounds in this organelle were studied in the salivary gland cells of Drosophila during larval and prepupal development [3].
• The copper-containing granules in the mid-gut epithelium of larval Drosophila melanogaster were examined for acid phosphatase by combined histochemistry and energy-dispersive, X-ray microanalysis [4].
• Excess of nonsynonymous polymorphism at Acph-1 in different gene arrangements of Drosophila subobscura [5].

Biological context of Acph-1

• Strong genetic differentiation at Acph-1 was detected among the different arrangements, which is reflected in strong linkage disequilibrium between the variants at informative polymorphic sites and the type of arrangement [5].
• The pattern of variation at Acph-1 within the young arrangements is unusual for nuclear genes and has the same characteristics previously detected in most genes of the mitochondrial genome [5].
• Lines differ in their chromosomal arrangement for segment I of the O chromosome (O(st) and O(3+4)) and in the Acph-1 electrophoretic allele (Acph-1(100), Acph-1(054), and Acph-1(>100)) [6].
• Amino acid sequence comparisons have revealed several highly conserved motifs between Drosophila Acph-1 and vertebrate lysosomal and prostatic acid phosphatases [7].
• We have isolated and purified two parental homodimers and a unique heterodimer of acid phosphatase [coded by Acph-1 (1.05)(F) and Acph-1 (0.95)(S)] from isogenic homozygotes and heterozygotes of Drosophila malerkotliana [8].

Anatomical context of Acph-1

• Vitamin A deprivation and genetic elimination of the lysosomal enzyme acid phosphatase alsoprotect the photoreceptors of rdgB flies against light-induced damage [9].
• Ultrastructural histochemical localization of acid phosphatase in salivary glands of Drosophila melanogaster [10].
• When glands having wild-type acid phosphatase activity are incubated for this time, deposition of the final reaction product is observed in essentially every lysosome and artifactual staining is minimal [10].
• These acid phosphatase-positive vacuoles also contained both types of abnormal granules [11].
• With regard to cellular degeneration and the involvement of lysosomes, we conclude that mechanisms other than gross modification of the lipid and/or lipid/protein ratio of their membranes are involved in the liberation of the acid phosphatase contents [12].

Associations of Acph-1 with chemical compounds

• Trypan blue stains a patch of dead cells localized to the wing pouch of imaginal discs and the same area shows acid phosphatase (AcPase) activity suggesting that the cell death is lysosomal [13].
• A trace of hexosamines, but no N-acetylneuraminic acid, was found in the ACPH allozymes [14].
• Histochemical techniques were used to characterize these two classes of muscle according to their content of various enzymes (alpha-glycerophosphate, NAD-dependent isocitrate, malate and succinate dehydrogenases, fumarase, acid phosphatase, adenosine triphosphatase and acetylcholinesterase) and of glycogen [15].

Other interactions of Acph-1

• Unlike in D. melanogaster and D. simulans, levels of variation at the y gene region of D. subobscura are not reduced relative to those found at other genomic regions in the same species (rp49, Acp70A, and Acph-1) [16].
• Three enzymes (acid phosphatase, peroxidase, and tyrosinase) are localized within the eyes of wild type and white mutant Drosophila melanogaster by electron microscopy [11].
• Synchronous allelic expression at esterase & acid phosphatase loci in interspecific Drosophila hybrids [17].
• We have investigated 13 alleles of four genes coding for acid phosphatase, alpha- and beta-esterases, and malic enzyme [18].

Analytical, diagnostic and therapeutic context of Acph-1

• Pure acid phosphatase-1 displays heterogeneity in isoelectric focusing, with a major band at pH 5 [2].
• Rocket immunoelectrophoresis was used to correlate the levels of acid phosphatase activity and protein in flies containing the deficiency [19].
• Immunological titration experiments showed that the variant enzymes had the same enzyme activity per molecule of ACPH as the wild-type enzyme [20].
• Molecular cloning, sequencing and alternative splicing of the acid phosphatase (Acph) gene from Drosophila virilis [21].

References