The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Ssbp1  -  single-stranded DNA binding protein 1,...

Rattus norvegicus

Synonyms: Mt-SSB, MtSSB, Single strand DNA-binding protein P16, Single-stranded DNA-binding protein, mitochondrial, Ssbp
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Ssbp1


Psychiatry related information on Ssbp1

  • The levels of CORT after sleep deprivation in P16, P20 and P24 animals were similar, resulting in an age-dependent decrease of the magnitude of the response [6].

High impact information on Ssbp1

  • Analysis of subcellular fractions revealed that nearly all of the cellular P16 was located in the mitochondria and that only trace amounts of protein of comparable electrophoretic mobility could be isolated from the nuclear or cytoplasmic fractions [7].
  • Purification and general properties of the DNA-binding protein (P16) from rat liver mitochondria [7].
  • These results indicate that P16 is synthesized on cytoplasmic ribosomes and imported into the mitochondria [7].
  • The labeling of P16 with [35S]methionine in primary rat hepatocyte cultures was inhibited by more than 90% by the cytoplasmic translation inhibitor cycloheximide, but unaffected by the mitochondrial-specific agent chloramphenicol [7].
  • Inhibition of the synthesis of this single-stranded DNA binding protein after 48 hr of incubation with NGF is potentiated by concomitant treatment of PC12 cells with antimitotic drugs acting at different levels of DNA replication [2].

Biological context of Ssbp1


Anatomical context of Ssbp1

  • The Purkinje cells, glomeruli, and Golgi cells began to be stained with MAb 6B4 from P10, P16, and P20, respectively [12].
  • Compared with that in nondegenerative Sprague-Dawley (SD) rats, the rate of photoreceptor death is abnormally high from P16 and remains several-fold higher than normal into young adulthood [13].
  • (-)-Nicotine exposure during P8 to P16 resulted in a significant and long-lasting increase in the number of nAChR isoforms labeled by (-)-[3H]nicotine, but not by [3H]epibatidine, in the cortex, hippocampus, and striatum of adult rat [14].
  • Levels of all the neutrinin proteins in rat cerebral cortex, as represented by P12, P15, and P16, showed an ontogenetic increase from the early postnatal days to the adult [15].
  • Because P12, P15, P16, and P20 were neutral proteins (pl 7.0) and specifically distributed in neuronal membranes, the new family of membrane-associated PKC substrate proteins was referred to as neutrinins [15].

Associations of Ssbp1 with chemical compounds


Regulatory relationships of Ssbp1


Other interactions of Ssbp1


Analytical, diagnostic and therapeutic context of Ssbp1

  • The gene for the rat mitochondrial single-stranded DNA-binding protein (mtSSB) was amplified by PCR and isolated as several overlapping genomic clones [18].
  • Measurement of the affinity of P16 for isolated sites by titration with either oligo(dT)8 or 5'-dephosphorylated oligo(dT)8 indicated values on the order of 10(7) M-1 [1].
  • Amino-terminal sequence analysis of P16 and a protease-insensitive, DNA binding domain (Mr approximately 6000) revealed that the DNA binding domain residues, at least in part, in the amino-terminal third of the P16 molecule [1].
  • The mitochondrial DNA-binding protein P16 was isolated from rat liver mitochondrial lysates by affinity chromatography on single strand DNA agarose and separated from DNA in the preparation by alkaline CsCl isopycnic gradients [7].
  • Analysis of chemically fixed complexes by CsCl isopycnic gradient centrifugation showed that P16 was bound to a large subpopulation of mtDNA enriched in displacement loops (D-loops) [19].


  1. Structural and functional studies of the rat mitochondrial single strand DNA binding protein P16. Hoke, G.D., Pavco, P.A., Ledwith, B.J., Van Tuyle, G.C. Arch. Biochem. Biophys. (1990) [Pubmed]
  2. Nerve growth factor inhibits the synthesis of a single-stranded DNA binding protein in pheochromocytoma cells (clone PC12). Biocca, S., Cattaneo, A., Calissano, P. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  3. Phosphotyrosine-containing lactate dehydrogenase is restricted to the nuclei of PC12 pheochromocytoma cells. Zhong, X.H., Howard, B.D. Mol. Cell. Biol. (1990) [Pubmed]
  4. A novel single-stranded DNA-binding protein from the Novikoff hepatoma which stimulates DNA polymerase beta. Purification and general characterization. Koerner, T.J., Meyer, R.R. J. Biol. Chem. (1983) [Pubmed]
  5. The polypeptide P16 is a carboxy-terminal cleavage product of rat growth hormone in anterior pituitary and GH3 pituitary tumor cells. Davis, R.B., Morris, J., Ivarie, R. Mol. Endocrinol. (1987) [Pubmed]
  6. Sleep deprivation elevates plasma corticosterone levels in neonatal rats. Hairston, I.S., Ruby, N.F., Brooke, S., Peyron, C., Denning, D.P., Heller, H.C., Sapolsky, R.M. Neurosci. Lett. (2001) [Pubmed]
  7. Purification and general properties of the DNA-binding protein (P16) from rat liver mitochondria. Pavco, P.A., Van Tuyle, G.C. J. Cell Biol. (1985) [Pubmed]
  8. Puralpha, a single-stranded deoxyribonucleic acid binding protein, augments placental lactogen gene transcription. Limesand, S.W., Jeckel, K.M., Anthony, R.V. Mol. Endocrinol. (2004) [Pubmed]
  9. Genesis and fate of the perireticular thalamic nucleus during early development. Earle, K.L., Mitrofanis, J. J. Comp. Neurol. (1996) [Pubmed]
  10. Puralpha, a single-stranded DNA binding protein, suppresses the enhancer activity of cAMP response element (CRE). Sadakata, T., Kuo, C., Ichikawa, H., Nishikawa, E., Niu, S.Y., Kumamaru, E., Miki, N. Brain Res. Mol. Brain Res. (2000) [Pubmed]
  11. Internucleosomal DNA fragmentation during deprived and non-deprived olfactory development. Najbauer, J., Yan, X.X., Leon, M. Brain Res. (2002) [Pubmed]
  12. A chondroitin sulfate proteoglycan that is developmentally regulated in the cerebellar mossy fiber system. Maeda, N., Matsui, F., Oohira, A. Dev. Biol. (1992) [Pubmed]
  13. Photoreceptor death, trophic factor expression, retinal oxygen status, and photoreceptor function in the P23H rat. Yu, D.Y., Cringle, S., Valter, K., Walsh, N., Lee, D., Stone, J. Invest. Ophthalmol. Vis. Sci. (2004) [Pubmed]
  14. Nicotine exposure during a critical period of development leads to persistent changes in nicotinic acetylcholine receptors of adult rat brain. Miao, H., Liu, C., Bishop, K., Gong, Z.H., Nordberg, A., Zhang, X. J. Neurochem. (1998) [Pubmed]
  15. Novel endogenous protein kinase C substrate proteins, neutrinins, in brain synaptic membranes of maturing rat. Chen, X.F., Miao, H.H., Zhang, J.W., Han, Y., Du, Y.C. J. Neurochem. (1996) [Pubmed]
  16. Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch. Ito, K., Sato, K., Endo, H. Nucleic Acids Res. (1994) [Pubmed]
  17. Identification of a single-stranded DNA binding protein from rat liver with high mobility group protein 1. Bonne, C., Sautiere, P., Duguet, M., de Recondo, A.M. J. Biol. Chem. (1982) [Pubmed]
  18. The gene and processed pseudogenes of the rat mitochondrial single-strand DNA-binding protein: structure and promoter strength analyses. Gupta, S., Van Tuyle, G.C. Gene (1998) [Pubmed]
  19. The rat liver mitochondrial DNA-protein complex: displaced single strands of replicative intermediates are protein coated. Van Tuyle, G.C., Pavco, P.A. J. Cell Biol. (1985) [Pubmed]
WikiGenes - Universities