Disease relevance of EFCBP1

• High level expression in Escherichia coli and characterization of the EF-hand calcium-binding protein caltractin [1].
• Head and neck squamous cell carcinoma express high levels of the EF-hand calcium-binding protein S100A2 in contrast to other tumorigenic tissues and cell lines where the expression of this protein is reduced [2].
• Recently, profilin and 2 EF-hand calcium-binding protein (polcalcin) have been shown to play a role in chenopod pollinosis [3].
• NMR assignments, secondary structure, and global fold of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea [4].
• The presence of the EF-hand-calcium-binding protein S100A4 in the carcinoma cells of the primary tumour is associated with a shorter survival time of a group of breast cancer patients [5].

High impact information on EFCBP1

• This gene, Necab, encodes a putative cytoplasmic Ca(2+)-binding protein and coincides with Pax6 expression pattern in the neural ectoderm of the optic vesicle and in the forebrain pretectum [6].
• S100B is a small, dimeric EF-hand calcium-binding protein abundant in vertebrates [7].
• Calbrain, a novel two EF-hand calcium-binding protein that suppresses Ca2+/calmodulin-dependent protein kinase II activity in the brain [8].
• Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes [9].
• RESULTS: The isoelectric point (pI) 4.7 form of carp parvalbumin, a three EF-hand calcium-binding protein, was purified to homogeneity [10].

Biological context of EFCBP1

• Human EFCBP1 encodes a 351 amino acid protein containing two EF-hand motifs and an antibiotic biosynthesis monooxygenase (ABM) domain, sharing 49.9 and 56.8% global homology with human EFCBP2 and EFCBP3 [11].
• In addition, we found that EFCBP1, EFCBP2 and EFCBP3 share a similar exon distribution mode, though their chromosomal localizations, genomic sizes and intron sizes are diverse [11].
• Furthermore, comparison of the structure of Calsensin with other members of the EF-hand calcium-binding protein family provides insight into plausible mechanisms of calcium and target protein binding [12].
• The EF-hand calcium-binding protein from Saccharopolyspora erythraea has been shown, using 113Cd NMR, to possess three Cd(2+)-ion binding sites [13].
• Calcium- and integrin-binding protein (CIB) is a small EF-hand calcium-binding protein that is involved in hemostasis through its interaction with the alphaIIb cytoplasmic domain of integrinalphaIIbbeta(3) [14].

Anatomical context of EFCBP1

• The expression level of EFCBP1 equals in putamen and cerebral cortex, while no hybridization signal was detected in spinal cord [11].
• EFCBP1/NECAB1, a brain-specifically expressed gene with highest abundance in temporal lobe, encodes a protein containing EF-hand and antibiotic biosynthesis monooxygenase domains [11].
• Ionized calcium binding adaptor molecule 1, Iba1, is an EF hand calcium binding protein whose expression is restricted to macrophages/microglia [15].
• Consequently, we hypothesized that the somal volume of another subset of pyramidal cells immunoreactive for neuronal calcium binding protein-1 (Necab-1) is significantly reduced in schizophrenia [16].
• Calsensin is an EF-hand calcium-binding protein expressed by a subset of peripheral sensory neurons that fasciculate into a single tract in the leech central nervous system [12].

Associations of EFCBP1 with chemical compounds

• RESULTS: The allergen profile of ash pollen comprises Fra e 1, a major allergen related to the major olive allergen, Ole e 1, and to group 11 grass pollen allergens, the panallergen profilin, a two EF-hand calcium-binding protein, a pectinesterase-like molecule and an allergen sharing epitopes with group 4 grass pollen allergens [17].
• Taken together, these findings demonstrate in zebrafish and rainbow trout that dioxin increases expression of this EF-hand calcium-binding protein gene in a tissue-dependent fashion [18].

Physical interactions of EFCBP1

• The crystal structure of human EF-hand calcium-binding protein S100A12 in its calcium-bound form has been determined to 1.95 A resolution by molecular replacement using the structure of the S100B protein [19].

Other interactions of EFCBP1

• S100A11 is a homodimeric EF-hand calcium binding protein that undergoes a calcium-induced conformational change and interacts with the phospholipid binding protein annexin I to coordinate membrane association [20].
• A role for EF-hand calcium-binding protein Mts1 (S100A4) in the phosphorylation and the assembly of myosin filaments was studied [21].
• Centrin is an EF-hand calcium-binding protein found in microtubule organizing centers of organisms ranging from algae and yeast to man [22].
• The results show that, although its function remains undetermined, calretinin is the major EF-hand calcium-binding protein in the frog retina and optic tectum [23].

Analytical, diagnostic and therapeutic context of EFCBP1

• Sequence analysis revealed 99% homology to the EF-hand calcium-binding protein S100P (Placental) [24].
• By immunocytochemistry the distribution and developmental expression of the small EF-hand calcium-binding protein calsensin in the peripheral (PNS) and central nervous system (CNS) of the three hirudinid leech species Haemopis, Hirudo, and Macrobdella was compared [25].

References