The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

SLBP  -  stem-loop binding protein

Homo sapiens

Synonyms: HBP, Histone RNA hairpin-binding protein, Histone stem-loop-binding protein
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of SLBP

  • Using hairpin II of U1 small nuclear RNA (U1hpII) or the 3' stem loop of histone mRNA as bait, we could selectively amplify T7 phage that display either the spliceosomal protein U1A or the histone stem loop-binding protein from a lung cDNA phage library containing more than 10(7) independent clones [1].
  • Analysis of tissue biopsies from patients with necrotizing fasciitis revealed recruitment of neutrophils and monocytes to the infectious site, combined with the release of HBP [2].
  • The results show that M protein, in synergy with HBP, evokes an inflammatory response that may contribute to the profound pathophysiological consequences seen in severe streptococcal infections [2].
  • We hypothesized a stepwise increase in these platelet activation indices between healthy controls (HC, n = 60), 'high-risk' essential hypertensive subjects (HBP, n = 45) and treated, previously diagnosed patients with malignant phase hypertension (MHT, n = 45) [3].
  • No HBP has been described in Brucella spp [4].

High impact information on SLBP

  • The stem-loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells [5].
  • SLBP binds histone pre-mRNAs and facilitates 3'-end processing by promoting stable association of U7 snRNP with the pre-mRNA [5].
  • Translation of the HBP open reading frame in vitro produced a 43 kDa protein with RNA binding specificity identical to murine or bovine HBP [6].
  • The HBP gene is composed of eight exons covering 19.5 kb on the short arm of chromosome 4 [6].
  • Drosophilia SLBP is heavily phosphorylated, containing up to seven phosphoryl groups [7].

Biological context of SLBP


Anatomical context of SLBP

  • T230 is highly conserved; we have determined that this site is also completely phosphorylated in baculovirus-expressed mammalian SLBP and extensively phosphorylated in both Drosophila and mammalian cultured cells [7].
  • In Xenopus, there are two SLBPs: xSLBP1, the homologue of the mammalian SLBP, which is required for processing of histone pre-mRNA, and xSLBP2, which is expressed only during oogenesis and is bound to the stored histone mRNA in Xenopus oocytes [10].
  • Only 5-10% of the SLBP could be extracted from the polyribosomes with salt [11].
  • In the nucleus, both U7 snRNP and SLBP are present in coiled bodies, structures that are associated with histone genes and may play a direct role in histone pre-mRNA processing in vivo [12].
  • Heparin-binding proteins (HBP) from seminal plasma have been expected to participate in modulation of the acrosomal reaction, and have been correlated with fertility in some species [13].

Associations of SLBP with chemical compounds

  • On the other hand, the tendency for the anaerobic biodegradability was; BPF > HBP > BPS, BPA, TDP > BPE > BPB [14].

Physical interactions of SLBP

  • The cleavage of histone pre-mRNA to form the unique 3' end requires the U7 snRNP and the stem-loop binding protein (SLBP) that binds the 3' end of histone mRNA [15].

Regulatory relationships of SLBP

  • Removal of SLBP by RNA interference causes an increase in the number of cells in S-phase and this effect can be reversed by expressing an exogenous SLBP resistant to the small interfering RNA [16].
  • These data indicate that SLBP stimulates the translation of histone mRNAs through a functional interaction with both the mRNA stem-loop and the 5' cap that is mediated by eIF4G and eIF3 [17].

Other interactions of SLBP

  • It is likely that the RBD of SLBP interacts directly with both the stem-loop RNA and other processing factor(s), most likely the U7 snRNP, to facilitate histone pre-mRNA processing [18].
  • We previously identified a 100-kDa zinc finger protein (ZFP100) as a component of U7 snRNP that interacts with the SLBP/pre-mRNA complex [19].

Analytical, diagnostic and therapeutic context of SLBP

  • To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait [5].
  • We also analyze the messages bound to the endogenous SLBP on polyribosomes by immunoprecipitation [20].
  • Electrophoretic mobility shift assays demonstrated that the SLBP-induced opening of HDE actually facilitates U7 snRNA anchoring on the histone H4-12 pre-mRNAs 3' end [21].
  • OBJECTIVES: (1) To compare blood pressure (BP) readings with an automated arm cuff oscillometric device (AutoBP) to readings with a mercury sphygmomanometer (HgBP) and (2) to evaluate the impact on the prevalence of hypertension (HBP) in a population-based survey [22].


  1. T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries. Danner, S., Belasco, J.G. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  2. Streptococcal M protein: a multipotent and powerful inducer of inflammation. Påhlman, L.I., Mörgelin, M., Eckert, J., Johansson, L., Russell, W., Riesbeck, K., Soehnlein, O., Lindbom, L., Norrby-Teglund, A., Schumann, R.R., Björck, L., Herwald, H. J. Immunol. (2006) [Pubmed]
  3. Assessment of platelet activation indices using the ADVIATM 120 amongst 'high-risk' patients with hypertension. Boos, C.J., Beevers, G.D., Lip, G.Y. Ann. Med. (2007) [Pubmed]
  4. Brucella outer membrane protein Omp31 is a haemin-binding protein. Delpino, M.V., Cassataro, J., Fossati, C.A., Goldbaum, F.A., Baldi, P.C. Microbes Infect. (2006) [Pubmed]
  5. A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3'-end processing. Dominski, Z., Erkmann, J.A., Yang, X., Sànchez, R., Marzluff, W.F. Genes Dev. (2002) [Pubmed]
  6. The gene for histone RNA hairpin binding protein is located on human chromosome 4 and encodes a novel type of RNA binding protein. Martin, F., Schaller, A., Eglite, S., Schümperli, D., Müller, B. EMBO J. (1997) [Pubmed]
  7. Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding. Borchers, C.H., Thapar, R., Petrotchenko, E.V., Torres, M.P., Speir, J.P., Easterling, M., Dominski, Z., Marzluff, W.F. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  8. Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, at the end of S phase. Zheng, L., Dominski, Z., Yang, X.C., Elms, P., Raska, C.S., Borchers, C.H., Marzluff, W.F. Mol. Cell. Biol. (2003) [Pubmed]
  9. Nuclear import of the stem-loop binding protein and localization during the cell cycle. Erkmann, J.A., Wagner, E.J., Dong, J., Zhang, Y., Kutay, U., Marzluff, W.F. Mol. Biol. Cell (2005) [Pubmed]
  10. The stem-loop binding protein is required for efficient translation of histone mRNA in vivo and in vitro. Sànchez, R., Marzluff, W.F. Mol. Cell. Biol. (2002) [Pubmed]
  11. Efficient extraction and partial purification of the polyribosome-associated stem-loop binding protein bound to the 3' end of histone mRNA. Hanson, R.J., Sun, J., Willis, D.G., Marzluff, W.F. Biochemistry (1996) [Pubmed]
  12. Formation of the 3' end of histone mRNA. Dominski, Z., Marzluff, W.F. Gene (1999) [Pubmed]
  13. Herapin-binding proteins of canine seminal plasma. de Souza, F.F., Martins, M.I., dos Santos Fernandes, C.E., Ribolla, P.E., Lopes, M.D. Theriogenology (2006) [Pubmed]
  14. Biodegradation of a variety of bisphenols under aerobic and anaerobic conditions. Ike, M., Chen, M.Y., Danzl, E., Sei, K., Fujita, M. Water Sci. Technol. (2006) [Pubmed]
  15. ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase. Wagner, E.J., Marzluff, W.F. Mol. Cell. Biol. (2006) [Pubmed]
  16. Expression of an RNAi-resistant SLBP restores proper S-phase progression. Wagner, E.J., Berkow, A., Marzluff, W.F. Biochem. Soc. Trans. (2005) [Pubmed]
  17. The histone 3'-terminal stem-loop-binding protein enhances translation through a functional and physical interaction with eukaryotic initiation factor 4G (eIF4G) and eIF3. Ling, J., Morley, S.J., Pain, V.M., Marzluff, W.F., Gallie, D.R. Mol. Cell. Biol. (2002) [Pubmed]
  18. Mutations in the RNA binding domain of stem-loop binding protein define separable requirements for RNA binding and for histone pre-mRNA processing. Dominski, Z., Erkmann, J.A., Greenland, J.A., Marzluff, W.F. Mol. Cell. Biol. (2001) [Pubmed]
  19. Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein. Wagner, E.J., Ospina, J.K., Hu, Y., Dundr, M., Matera, A.G., Marzluff, W.F. RNA (2006) [Pubmed]
  20. Genome-wide analysis of mRNAs bound to the histone stem-loop binding protein. Townley-Tilson, W.H., Pendergrass, S.A., Marzluff, W.F., Whitfield, M.L. RNA (2006) [Pubmed]
  21. Binding of human SLBP on the 3'-UTR of histone precursor H4-12 mRNA induces structural rearrangements that enable U7 snRNA anchoring. Jaeger, S., Martin, F., Rudinger-Thirion, J., Gieg??, R., Eriani, G. Nucleic Acids Res. (2006) [Pubmed]
  22. Difference in Blood Pressure Readings with Mercury and Automated Devices: Impact on Hypertension Prevalence Estimates in Dar es Salaam, Tanzania. Chiolero, A., Gervasoni, J.P., Rwebogora, A., Balampama, M., Paccaud, F., Bovet, P. Eur. J. Epidemiol. (2006) [Pubmed]
WikiGenes - Universities