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Gene Review

ERG3  -  C-5 sterol desaturase

Saccharomyces cerevisiae S288c

Synonyms: Ergosterol Delta(5,6) desaturase, L2150, PSO6, SYR1, Sterol-C5-desaturase, ...
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Disease relevance of ERG3


High impact information on ERG3


Biological context of ERG3

  • Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility [5].
  • We demonstrate that mutations affecting early and late steps in sterol homeostasis modulate the expression of the ERG3 gene: a late step in sterol biosynthesis in yeast [6].
  • Our results demonstrate that the absence of sterol esterification leads to a decrease in total intracellular sterol and ERG3 is a target of this negative regulation [6].
  • Deletion analysis of the ERG3 promoter identified two upstream activation sequences: UAS1 which when deleted reduces ERG3 gene expression 3-4-fold but maintains sterol regulation and UAS2, which when deleted further reduces ERG3 expression and abolishes sterol regulation [6].
  • While all mutants of the DNA repair group and pso3 were sensitive to DEO and NDEA the pso6 mutant revealed WT or near WT resistance to these mutagens [7].

Anatomical context of ERG3

  • Mutation of ERG3, encoding another ergosterol biosynthetic enzyme, also caused Nal sensitivity, suggesting that plasma membrane sterol composition, and plasma membrane function, mediates recovery from Nal-mediated inhibition of Start [8].
  • These results indicate that mitochondria function as important physiological partners with Erg3p in the accumulation of toxic sterol intermediates in the presence of azoles [9].

Associations of ERG3 with chemical compounds

  • The data suggest that the conserved hydroxyl function at position 114 in the ERG3 family makes the abstraction of the 6alpha-hydrogen atom substantially less rate-limiting during the 5-DES reaction [10].
  • Insertionally inactivated mutants of ERG3 fail to grow without 'sparking' levels of delta 5 sterols in heme-deficient cells, and are unable to grow on the respiratory substrates glycerol and ethanol [11].
  • ERG3 is the structural gene in Saccharomyces cerevisiae for the sterol delta 5 desaturase that introduces the C5 = 6 unsaturation in ergosterol biosynthesis [11].
  • The yeast ORFs YDL139c, YDL147w, ERG3 and ERG11 were tagged with sequences encoding the hormone-binding domains of mammalian steroid receptors by PCR-mediated, targeted integration into the yeast genome [12].
  • Further transcript analysis showed that expression of the ERG3 gene, which encodes C-5 sterol desaturase, was reduced in two amphotericin B-resistant isolates [13].

Other interactions of ERG3

  • Northern (RNA) analysis revealed that the single-deletion mutants had a marked increase in message for the undeleted ERG3 and ERG11 genes [5].
  • Expression of ERG3 is increased in response to a mutation in the major isoform of HMG CoA reductase which catalyzes the rate-limiting step of sterol biosynthesis [6].
  • The ERG3 gene has been mapped on chromosome XII, 13.7 centimorgans from GAL2 toward SPT8 [11].
  • Erg2p and Erg3p, two enzymes catalyzing sequential reactions also appear to have different interaction partners [14].
  • Both predicted proteins consist of 258 amino acid residues (77% identity) which show sequence similarity to di-iron-binding enzymes, such as Sur2p and Erg3p from yeast, involved in oxygen-dependent lipid modifications [15].

Analytical, diagnostic and therapeutic context of ERG3

  • Northern blot analysis using the ERG 3 probe showed a strong expression of ERG 3 in three of the seven transgenic calli [16].
  • The role of 15 residues in the reaction catalyzed by Arabidopsis thaliana Delta7-sterol-C5(6)-desaturase (5-DES) was investigated using site-directed mutagenesis and expression of the mutated enzymes in an erg3 yeast strain defective in 5-DES [17].
  • The protein product of SYR1 was identified by Western blot analysis as a protein of 40 kDa in the particulate fraction [18].


  1. Allelism of Saccharomyces cerevisiae genes PSO6, involved in survival after 3-CPs+UVA induced damage, and ERG3, encoding the enzyme sterol C-5 desaturase. Schmidt, C.L., Grey, M., Schmidt, M., Brendel, M., Henriques, J.A. Yeast (1999) [Pubmed]
  2. Transcriptional regulation by ergosterol in the yeast Saccharomyces cerevisiae. Smith, S.J., Crowley, J.H., Parks, L.W. Mol. Cell. Biol. (1996) [Pubmed]
  3. Isolation and characterization of an Arabidopsis thaliana cDNA encoding a delta 7-sterol-C-5-desaturase by functional complementation of a defective yeast mutant. Gachotte, D., Husselstein, T., Bard, M., Lacroute, F., Benveniste, P. Plant J. (1996) [Pubmed]
  4. Dual effects of plant steroidal alkaloids on Saccharomyces cerevisiae. Simons, V., Morrissey, J.P., Latijnhouwers, M., Csukai, M., Cleaver, A., Yarrow, C., Osbourn, A. Antimicrob. Agents Chemother. (2006) [Pubmed]
  5. Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility. Geber, A., Hitchcock, C.A., Swartz, J.E., Pullen, F.S., Marsden, K.E., Kwon-Chung, K.J., Bennett, J.E. Antimicrob. Agents Chemother. (1995) [Pubmed]
  6. Positive and negative regulation of a sterol biosynthetic gene (ERG3) in the post-squalene portion of the yeast ergosterol pathway. Arthington-Skaggs, B.A., Crowell, D.N., Yang, H., Sturley, S.L., Bard, M. FEBS Lett. (1996) [Pubmed]
  7. Further phenotypic characterization of pso mutants of Saccharomyces cerevisiae with respect to DNA repair and response to oxidative stress. Pungartnik, C., Picada, J., Brendel, M., Henriques, J.A. Genet. Mol. Res. (2002) [Pubmed]
  8. Mutations sensitizing yeast cells to the start inhibitor nalidixic acid. Prendergast, J.A., Singer, R.A., Rowley, N., Rowley, A., Johnston, G.C., Danos, M., Kennedy, B., Gaber, R.F. Yeast (1995) [Pubmed]
  9. Modulation of fluconazole sensitivity by the interaction of mitochondria and erg3p in Saccharomyces cerevisiae. Kontoyiannis, D.P. J. Antimicrob. Chemother. (2000) [Pubmed]
  10. Deuterated delta 7-cholestenol analogues as mechanistic probes for wild-type and mutated delta 7-sterol-C5(6)-desaturase. Rahier, A. Biochemistry (2001) [Pubmed]
  11. The ERG3 gene in Saccharomyces cerevisiae is required for the utilization of respiratory substrates and in heme-deficient cells. Smith, S.J., Parks, L.W. Yeast (1993) [Pubmed]
  12. Post-translational regulation of Saccharomyces cerevisiae proteins tagged with the hormone-binding domains of mammalian nuclear receptors. Launhardt, H., Munder, T. Mol. Gen. Genet. (2000) [Pubmed]
  13. Disruption of ergosterol biosynthesis confers resistance to amphotericin B in Candida lusitaniae. Young, L.Y., Hull, C.M., Heitman, J. Antimicrob. Agents Chemother. (2003) [Pubmed]
  14. A systematic study of yeast sterol biosynthetic protein-protein interactions using the split-ubiquitin system. Mo, C., Bard, M. Biochim. Biophys. Acta (2005) [Pubmed]
  15. Functional characterization of sphingolipid C4-hydroxylase genes from Arabidopsis thaliana. Sperling, P., Ternes, P., Moll, H., Franke, S., Zähringer, U., Heinz, E. FEBS Lett. (2001) [Pubmed]
  16. An Arabidopsis mutant deficient in sterol biosynthesis: heterologous complementation by ERG 3 encoding a delta 7-sterol-C-5-desaturase from yeast. Gachotte, D., Meens, R., Benveniste, P. Plant J. (1995) [Pubmed]
  17. Role of highly conserved residues in the reaction catalyzed by recombinant Delta7-sterol-C5(6)-desaturase studied by site-directed mutagenesis. Taton, M., Husselstein, T., Benveniste, P., Rahier, A. Biochemistry (2000) [Pubmed]
  18. Identification and analysis of the Saccharomyces cerevisiae SYR1 gene reveals that ergosterol is involved in the action of syringomycin. Taguchi, N., Takano, Y., Julmanop, C., Wang, Y., Stock, S., Takemoto, J., Miyakawa, T. Microbiology (Reading, Engl.) (1994) [Pubmed]
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