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Gene Review

KIF23  -  kinesin family member 23

Homo sapiens

Synonyms: CHO1, KNSL5, Kinesin-like protein 5, Kinesin-like protein KIF23, MKLP-1, ...
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Disease relevance of KIF23

  • Microinjection of CHO1, either as an ascites fluid or as purified IgM, results in mitotic inhibition in a stage-specific and dose-dependent manner [1].

High impact information on KIF23


Biological context of KIF23


Anatomical context of KIF23

  • Although relaxing furrows contained overlapping microtubules from opposing centrosomes, they lacked microtubule bundles as well as INCENP and CHO1 [9].
  • A monoclonal antibody raised against mitotic spindles isolated from CHO cells ([CHO1], Sellitto, C., and R. Kuriyama. 1988. J. Cell Biol. 106:431-439) identifies an epitope that resides on polypeptides of 95 and 105 kD and is localized in the spindles of diverse organisms [1].
  • The CHO1 antigen in HeLa cell extracts copurifies with taxol-stabilized microtubules [1].
  • Thus, this CHO1/MKLP1 fragment establishes a nonuniform MT polarity pattern and does so by a similar sequence of events as occurs with the dendrite, the antecedent of which is a short process with a uniform MT polarity orientation [10].
  • Through the use of a series of degenerate primers selected from consensus sequences of CHO-1 and CHT1, we generated two probes that were used to search a Limulus cDNA library produced from central nervous system (CNS) tissue [11].

Associations of KIF23 with chemical compounds

  • Here, we show that in C. elegans embryos and in cultured human cells, respectively, ZEN-4 and MKLP1 are phosphorylated by aurora B in vitro and in vivo on conserved C-terminal serine residues [12].
  • The diffusion coefficient and sedimentation coefficient were determined for the native CHO1 antigen by gel filtration and sucrose density gradient centrifugation, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)[13]
  • (2000. Nature Neurosci. 3, 120-125) recently reported the successful cloning of the choline co-transporter in Caenorhabditis elegans (CHO-1) and rat (CHT1) [11].
  • Performance time, running speed, blood glucose and blood lactate concentrations were evaluated during two 25-km treadmill time trial (trial 1 and trial 2) separated by 7 days in which two groups (CHO1 and CHO2) had a carbohydrate loading [14].

Physical interactions of KIF23

  • Further deletion mapping allowed the identification of an 88 amino acid Arf3 binding domain in the C-terminus of MKLP1 [7].
  • Cep55 directly binds to MKLP1 in vitro and associates with the MKLP1-MgcRacGAP centralspindlin complex in vivo [15].

Enzymatic interactions of KIF23

  • The mitotic kinase Cdk1/cyclin B phosphorylates the motor domain of ZEN-4 on a conserved site within a basic amino-terminal extension characteristic of the MKLP1 subfamily [16].

Other interactions of KIF23


Analytical, diagnostic and therapeutic context of KIF23

  • Two-hybrid assays of the C-terminal domain of MKLP1 with each of the human Arf isoforms revealed strong interaction with each [7].
  • These results demonstrate that CHO1 consists of functionally differentiated subregions that act in concert to ensure complete cell separation [18].
  • Immunofluorescence analysis of injected cells reveals that those which complete mitosis display normal localization of CHO1, whereas arrested cells show no specific localization of the CHO1 antigen within the spindle [1].


  1. A monoclonal antibody to a mitotic microtubule-associated protein blocks mitotic progression. Nislow, C., Sellitto, C., Kuriyama, R., McIntosh, J.R. J. Cell Biol. (1990) [Pubmed]
  2. Annexin 11 is required for midbody formation and completion of the terminal phase of cytokinesis. Tomas, A., Futter, C., Moss, S.E. J. Cell Biol. (2004) [Pubmed]
  3. Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. Neef, R., Preisinger, C., Sutcliffe, J., Kopajtich, R., Nigg, E.A., Mayer, T.U., Barr, F.A. J. Cell Biol. (2003) [Pubmed]
  4. CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis. Kuriyama, R., Gustus, C., Terada, Y., Uetake, Y., Matuliene, J. J. Cell Biol. (2002) [Pubmed]
  5. Nonuniform microtubular polarity established by CHO1/MKLP1 motor protein is necessary for process formation of podocytes. Kobayashi, N., Reiser, J., Kriz, W., Kuriyama, R., Mundel, P. J. Cell Biol. (1998) [Pubmed]
  6. Molecular interactions of Polo-like-kinase 1 with the mitotic kinesin-like protein CHO1/MKLP-1. Liu, X., Zhou, T., Kuriyama, R., Erikson, R.L. J. Cell. Sci. (2004) [Pubmed]
  7. Arf proteins bind to mitotic kinesin-like protein 1 (MKLP1) in a GTP-dependent fashion. Boman, A.L., Kuai, J., Zhu, X., Chen, J., Kuriyama, R., Kahn, R.A. Cell Motil. Cytoskeleton (1999) [Pubmed]
  8. Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells. Zhu, C., Bossy-Wetzel, E., Jiang, W. Biochem. J. (2005) [Pubmed]
  9. Cleavage furrows formed between centrosomes lacking an intervening spindle and chromosomes contain microtubule bundles, INCENP, and CHO1 but not CENP-E. Savoian, M.S., Earnshaw, W.C., Khodjakov, A., Rieder, C.L. Mol. Biol. Cell (1999) [Pubmed]
  10. Expression of a kinesin-related motor protein induces Sf9 cells to form dendrite-like processes with nonuniform microtubule polarity orientation. Sharp, D.J., Kuriyama, R., Baas, P.W. J. Neurosci. (1996) [Pubmed]
  11. Molecular cloning of a cDNA for a putative choline co-transporter from Limulus CNS. Wang, Y., Cao, Z., Newkirk, R.F., Ivy, M.T., Townsel, J.G. Gene (2001) [Pubmed]
  12. Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis. Guse, A., Mishima, M., Glotzer, M. Curr. Biol. (2005) [Pubmed]
  13. Heterogeneity and microtubule interaction of the CHO1 antigen, a mitosis-specific kinesin-like protein. Analysis of subdomains expressed in insect sf9 cells. Kuriyama, R., Dragas-Granoic, S., Maekawa, T., Vassilev, A., Khodjakov, A., Kobayashi, H. J. Cell. Sci. (1994) [Pubmed]
  14. The effect of a carbohydrate loading on running performance during a 25-km treadmill time trial by level of aerobic capacity in athletes. Sullo, A., Monda, M., Brizzi, G., Meninno, V., Papa, A., Lombardi, P., Fabbri, B. European review for medical and pharmacological sciences. (1998) [Pubmed]
  15. Cep55, a microtubule-bundling protein, associates with centralspindlin to control the midbody integrity and cell abscission during cytokinesis. Zhao, W.M., Seki, A., Fang, G. Mol. Biol. Cell (2006) [Pubmed]
  16. Cell cycle regulation of central spindle assembly. Mishima, M., Pavicic, V., Grüneberg, U., Nigg, E.A., Glotzer, M. Nature (2004) [Pubmed]
  17. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Mishima, M., Kaitna, S., Glotzer, M. Dev. Cell (2002) [Pubmed]
  18. Role of the midbody matrix in cytokinesis: RNAi and genetic rescue analysis of the mammalian motor protein CHO1. Matuliene, J., Kuriyama, R. Mol. Biol. Cell (2004) [Pubmed]
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