Disease relevance of Cryoultramicrotomy

• The special features of our protocol include one-step dehydration of tissue sections in 100% ethanol immediately after cryosectioning, a self-made T-shape plate (T plate) and "exclusion microdissection" procedure [1].

High impact information on Cryoultramicrotomy

• Immunogold localization of placental lactogen and the SBU-3 antigen by cryoultramicrotomy at implantation in the sheep [2].
• Nevertheless, according to GC-MS analysis combined with tangential cryosectioning, the distribution of ACC was found to be fairly uniform across the cambial-region tissues [3].
• Perfusion fixation with high amounts of glutaraldehyde and the use of cryoultramicrotomy caused remarkable changes in immunostaining versus formaldehyde/picric acid fixation [4].
• Presence of angiotensin II in the adult male rat anterior pituitary gland: immunocytochemical study after cryoultramicrotomy [5].
• Close examination of cells revealed that the periplasmic space was compressed during cryosectioning, a finding which provided supporting evidence that this space is filled by a compressible gel [6].

Biological context of Cryoultramicrotomy

• After excision and cryosectioning of the tumours, frozen tissue sections were incubated with [(125I)Tyr2]-alpha-MSH or [(125I)Tyr2,Nle4,D-Phe7]-alpha-MSH and specific alpha-MSH binding sites were visualized by subsequent autoradiography [7].

Anatomical context of Cryoultramicrotomy

• Mitochondrial Bcl-2 protein levels were determined in hepatocytes and SECs using cryosectioning immunogold labeling electron microscopy.Results [8].
• The presence of endogenous ANF has also been demonstrated in the exocrine pancreas by immunocytochemistry on ultra-thin sections obtained by cryoultramicrotomy [9].
• Immunoreactivity to endogenous calcitonin was studied in osteoblasts from calvaria of neonatal rats, by immunocytochemistry with the use of ultrathin sections obtained by cryoultramicrotomy [10].
• Sideways position of the temporomandibular joint disk: coronal cryosectioning of fresh autopsy specimens [11].
• We observed mouse corneal stroma after treatment with 20 mM adenosine 5'-triphosphate (ATP), thin-sectioned by cryoultramicrotomy, with a transmission electron microscope [12].

Associations of Cryoultramicrotomy with chemical compounds

• While TcpA cannot be detected by surface immunogold labelling in transmission electron microscope preparations, EDTA pretreatment facilitates immunofluorescent antibody labelling of whole cells, and ultrathin cryosectioning techniques confirm membrane and periplasmic accumulation of TcpA [13].
• CONCLUSION: Within 1 h allergen challenge has induced patchy damage sites where epithelial restitution is already advanced; although easily produced by cryosectioning frank denudation was not evident in whole-mount preparations [14].
• After aldehyde fixation, tissue samples were prepared by cryoultramicrotomy or embedding in acrylic resins [15].
• Novel applications of acrylamide for cryosectioning of isolated cells, tissues, and arthropods [16].
• Rat kidneys were fixed, cryoprotected by sucrose, frozen, sectioned by cryoultramicrotomy, and dried at room temperature [17].

Gene context of Cryoultramicrotomy

• Ultrathin sections, obtained by cryoultramicrotomy, were incubated with anti-SP serum [18].
• Cryosectioning and embedding by the PLT method both require the antigen to withstand some degree of fixation [19].
• The forces during cryosectioning were measured using miniature strain gauges attached to a load cell fitted to the drive arm of the Porter-Blum MT-2 cryomicrotome [20].
• In this study we investigated the distribution of collagen types I-V in the human iris at the fine-structural level using cryoultramicrotomy and London Resin White plastic embedding [21].
• Immunocytochemical and cryoultramicrotomy techniques were applied to label endogenous as well as intravenously injected calcitonin (CT) in rat kidney [22].

Analytical, diagnostic and therapeutic context of Cryoultramicrotomy

• We have compared mRNA detection after different methods of tissue preservation, freeze-drying, cryosectioning, and formaldehyde or methanol fixation [23].
• Matrix vesicles or structures similar in profile and location were identified in rat epiphyseal growth plate cartilages that had been subjected to freeze-substitution, cryoultramicrotomy, or ethylene glycol fixation [24].

References