Disease relevance of Necator

• Furthermore, increased chlorophenol production by overexpression of TfdA also had a negative effect on 2,4-D degradation by C. necator JMP134 and by a different host, Burkholderia xenovorans LB400, harboring plasmid pJP4 [1].
• Preliminary studies indicated that single-dose (500 mg) mebendazole gave disappointing results in the treatment of hookworm infections (Necator americanus) in Mali [2].
• HupE and UreH from Rhodopseudomonas palustris CGA009 and UreJ from Cupriavidus necator H16 were shown to mediate Ni(2+) transport upon heterologous production in E. coli [3].
• Cross-reactive IgG in most necatoriasis sera was effectively absorbed with 20 micrograms/ml extract of Necator americanus [4].

High impact information on Necator

• A novel crystal structure of Na-ASP-2, a PR-1 protein secreted by infective larvae of the human hookworm Necator americanus, has been solved to resolution limits of 1.68 A and to an R-factor of 17% using the recombinant protein expressed in and secreted by Pichia pastoris [5].
• Unfortunately, pyrantel pamoate is not a panacea and in the case of Necator it is not as effective as in the other roundworms [6].
• An accumulation of chlorophenol was found to inhibit chlorophenoxyacetate degradation, and inactivation of the tfdB genes enhanced the toxic effect of 2,4-DCP on C. necator cells [1].
• We investigated the molecular basis of resistance of the obligate biotrophic grape powdery mildew fungus Uncinula necator to sterol demethylation-inhibiting fungicides (DMIs) [7].
• The sensitivity of 91 single-spore field isolates of U. necator to triadimenol was assessed by using a leaf disc assay [7].

Biological context of Necator

• Metabolism of lipid peroxidation products by the gastro-intestinal nematodes Necator americanus, Ancylostoma ceylanicum and Heligmosomoides polygyrus [8].
• An Ichthyobodo species isolated from the skin of Atlantic salmon parr in freshwater is suggested to represent L. necator sensu stricto, while another species, showing particular affinity for the gills, infects salmon in both fresh- and seawater [9].
• After 4 years of mass chemotherapy with a single annual dose of albendazole 400 mg, health education and promotion of environmental health, the prevalence of Necator americanus in Wilayat Tagah, Dhofar, Oman was reduced from 40 to 1.3% and from 6 to 0%, respectively, among rural and urban school children [10].

Anatomical context of Necator

• Collagen-like proteins, thought to be responsible for maintaining the structural integrity of the nematode cuticle, were isolated from adult Necator americanus and shown to be susceptible to digestion by purified mast cell proteases [11].
• Macrophages isolated from fish suffering from a non-lethal Ichthyobodo necator infection displayed a highly increased ability to produce lysozyme in response to both beta-glucan and LPS [12].

Associations of Necator with chemical compounds

• This study evaluated the efficacy and tolerance of a single oral 400-mg dose of albendazole on Necator americanus larvae, and compared its efficacy when administered between meals or with a meal [13].
• A QTL for resistance to Uncinula necator was identified on linkage group 16, and QTLs for endurance to Plasmopara viticola on linkage groups 9 and 10 of 'Regent'. Additional QTLs for the onset of berry ripening ("veraison"), berry size and axillary shoot growth were identified [14].
• Cuticle samples have been prepared from the human parasitic nematode Necator americanus using detergent solubilisation with sodium dodecyl sulphate and subsequent reduction of disulphide bonds using 2-beta-mercaptoethanol [15].
• EPR spectrometry was used to investigate the effect of excretory/secretory product from Necator americanus on superoxide radical anions generated by xanthine/xanthine oxidase as a measure of excretory/secretory product superoxide dismutase activity [16].
• Ichthyobodo necator, Chilodonella hexasticha and Ichthyophthirius multifiliis infections were treated with formalin, salt and malachite green-formalin baths, respectively, whenever any parasites were found [17].

Gene context of Necator

• The acetylcholinesterase secreted by Necator americanus, the human hookworm, was similarly specifically labelled [18].
• Gamma-glutamyl transpeptidase activity was only detected in Moniezia expansa and Necator americanus [19].
• Na-ctl-2, a cDNA encoding a C-type lectin expressed exclusively in adult Necator americanus hookworms [20].
• The recognition of antigens on the surface of adult and L4 Necator americanus by human and hamster post-infection sera [21].
• Experimental and field studies on the viability of eggs, and the infectivity of third-stage larvae of the human hookworm Necator americanus recovered from the creek waters of the Niger Delta, Nigeria, and their epidemiological significance [22].

References