Disease relevance of Lactococcus lactis

• Sucrose-6-phosphate hydrolase from Lactococcus lactis subsp. lactis K1-23 (formerly Streptococcus lactis K1-23) has been purified 600-fold to electrophoretic homogeneity [1].
• A 4.4-kb XhoI fragment of Streptococcus lactis L13 (Z268) lactose plasmid pUCL13, containing the beta-D-phosphogalactoside galactohydrolase (P-beta Gal; EC 3.2.1.85)-coding gene has been cloned in Escherichia coli [2].
• Two defective transducing bacteriophages induced by exposure of Streptococcus lactis C2 to ultraviolet irradiation have been isolated and characterized [3].
• It was observed that Staphylococcus aureus and Streptococcus lactis, although killed by heat treatment, stained a bright orange when treated with acridine orange dye [4].
• In addition, the cloned 5.7-kbp SalI and 2.5-kbp SmaI-SalI DNA fragments failed to hybridize to chromosomal DNA from Bacillus subtilis, Streptococcus lactis, Streptococcus mutans, and Lactobacillus acidophilus as well as to DNA from a sucrose plasmid from Salmonella tennessee [5].

High impact information on Lactococcus lactis

• Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger [6].
• Streptococcus lactis metabolizes arginine via the arginine deiminase pathway to ornithine, CO2, NH3, and ATP [6].
• Membranes of Streptococcus lactis were solubilized with 1.1% octyl-beta-D-glucopyranoside in the presence of 0.37% acetone/ether-washed phospholipid from several sources [7].
• We confirmed that in starved, valinomycin-treated cells of Streptococcus lactis 7962, Tl+ ions distributed themselves across the bacterial membrane in response to the potassium diffusion potential [8].
• Again, no plasmid DNA was detectable in transconjugants, and the latter could donate tetracycline resistance to S. faecalis, S. sanguis, and Streptococcus lactis [9].

Chemical compound and disease context of Lactococcus lactis

• With this method, the frequencies of transfer of lactose metabolism from Streptococcus lactis ME2 to S. lactis LM2302 were comparable to those achieved with agar surface matings [10].
• Galactose fermentation by Streptococcus lactis and Streptococcus cremoris: pathways, products, and regulation [11].
• Use of 31P nuclear magnetic resonance spectroscopy and 14C fluorography in studies of glycolysis and regulation of pyruvate kinase in Streptococcus lactis [12].
• Some lactose-negative (Lac-) mutants of Streptococcus lactis C2 and ML3 exhibited development of very high level streptomycin resistance after incubation with subinhibitory concentrations of the drug for 18 to 22 h [13].
• Uptake and metabolism of sucrose by Streptococcus lactis [14].

Biological context of Lactococcus lactis

• Regulation of glycolysis and sugar phosphotransferase activities in Streptococcus lactis: growth in the presence of 2-deoxy-D-glucose [15].
• Phosphate/hexose 6-phosphate antiport in Streptococcus lactis [16].
• We investigated the molecular relationship between the 60-megadalton (Mdal) recombinant lactose plasmids in ML 3 x LM2301 lactose-positive (Lac+) transconjugants and the genetic material of Streptococcus lactis ML3 [17].
• Lactose metabolism in Streptococcus lactis: phosphorylation of galactose and glucose moieties in vivo [18].
• Lactose-negative (Lac-) mutants were isolated from a variant of Streptococcus lactis C2 in which the lactose plasmid had become integrated into the chromosome [19].

Anatomical context of Lactococcus lactis

• Colostrum was inoculated with Streptococcus lactis or yogurt culture or preserved with .1% (vol/vol) formalin in two separate experiments [20].

Gene context of Lactococcus lactis

• Regulation of arginine-ornithine exchange and the arginine deiminase pathway in Streptococcus lactis [21].
• Partial purification and some properties of phi C2(W) lysin, a lytic enzyme produced by phage-infected cells of Streptococcus lactis C2 [22].
• Pyruvate dehydrogenase activity was detected in whole cells but not in cell-free extracts of Streptococcus lactis [23].

Analytical, diagnostic and therapeutic context of Lactococcus lactis

• Molecular cloning of the lactose-metabolizing genes from Streptococcus lactis [24].
• A lactose-negative (Lac-), proteinase-negative (Prt-) mutant, designated C145 was isolated from Streptococcus lactis C2 after treatment with nitrosoguanidine and ultraviolet irradiation [25].
• Lactose (Lac) plasmid species in Streptococcus lactis C2 and S. cremoris B1 (30 and 37 megadaltons, respectively) were demonstrated upon examination of DNA from the cleared lysates by agarose gel electrophoresis [26].
• Restriction mapping was employed to characterize the 104-kilobase (kb) cointegrate lactose plasmids from 15 independent transconjugants derived from Streptococcus lactis ML3 as well as the 55-kb lactose plasmid ( pSK08 ) and a previously uncharacterized 48.4-kb plasmid ( pRS01 ) from S. lactis ML3 [27].
• Polyacrylamide gel electropheresis and thin layer chromatography (TLC) were applied to detect the fate of aflatoxin B-1 in milk fermented with an active culture of Streptococcus lactis (ATCC-11454) [28].

References