Disease relevance of NSC401568

• Thus, delaying the addition of DMAP after ionomycin decreases chromosomal abnormalities and produces a high proportion of activated oocytes suitable for ICSI [1].
• All the methylated bases found in Streptomyces RNA (1-methyladenine, N6-methyladenine, N6,N6-dimethyladenine and 7-methylguanine) are inhibited by this antibiotic [2].

High impact information on NSC401568

• Moreover, dimethylaminopurine treatment increased the expression of MAPK phosphatase-2, providing a possible mechanism of action of this compound [3].
• Since phosphorylation cascades are implicated in cytokine signaling, the effects of the protein kinase inhibitor dimethylaminopurine (DMAP) on TNF action in bovine aortic endothelial cells (BAEC) were investigated [4].
• Inhibition of tumor necrosis factor signal transduction in endothelial cells by dimethylaminopurine [4].
• We micromanipulated chromosomes in spermatocytes treated with DMAP to learn why maloriented chromosomes often fail to reorient [5].
• Micromanipulation experiments disclose that neither the loss of old nor the acquisition of new microtubules is sufficiently affected by DMAP to account for the indefinite persistence of malorientations [5].

Biological context of NSC401568

• DMAP affected PtK1 cells in mitosis as it did spermatocytes in meiosis: improper chromosome orientations persisted, leading to frequent errors in distribution [5].
• In any case, DMAP reveals interactions of one kinetochore with another, which, though ordinarily suppressed, have implications for normal mitosis [5].
• However, use of higher temperature for the cyclization of one of the chloropyrimidines led to the dimethylaminopurine analogue as a sole product, formed via nucleophilic displacement of the chloro group by dimethylamine generated from DMF [6].
• The results suggested that although DMAP enhanced development and higher cell number, due attention should be paid to abnormal ploidy in pig NT embryos [7].
• Activation of bovine oocytes to produce a single haploid pronucleus in preparation for intracytoplasmic sperm injection (ICSI) has been investigated with various combinations of ionomycin and 6-dimethylaminopurine (DMAP) [1].

Anatomical context of NSC401568

• Injected oocytes were activated by treatment with 5 microM ionomycin (5 min) and, after a 3-h interval, with 1.9 mM 6-dimethylaminopurine (DMAP) for 3 h [8].
• In contrast, nuclear transfer blastocyst rates per fused embryo were lower (P < 0.0001) using cytoplasts activated with DMAP [9].
• Pretreatment of fibroblasts with the Jun N-terminal kinase inhibitor dimethylaminopurine abolished TNF-alpha- and IL-1beta-stimulated ICAM-1 expression [10].
• Ionomycin combined with DMAP resulted in 49, 6 and 3% cleavage, morula and blastocyst rates, respectively, when oocytes were activated after 24 h maturation [11].
• Dimethylaminopurine inhibits metabolic effects of insulin in primary adipocytes [12].

Associations of NSC401568 with other chemical compounds

• Parthenogenetic activation with 5 microns ionomycin followed by a 4-hr incubation in 1.9 mM dimethylaminopurine results in > 80% activation, and antitubulin immunofluorescence microscopy demonstrates that initially disarrayed microtubules are observed and the some microtubules extend from the remnants of the second meiotic spindle [13].
• This phosphorylation was selectively suppressed by the kinase inhibitors wortmannin, dimethylaminopurine, and LY294002, with a dose response that in each case paralleled the inhibition of DNA end-joining [14].
• Parthenogenetic blastocyst yields were greater (P < 0.001) following activation with DMAP than CHX (59.7 +/- 5.1 vs. 31.4 +/- 4.5 [mean +/- SEM]) [9].
• The binding and reactions of ImpU and ImpA on montmorillonite are blocked by N6,N6-dimethyladenine and dA5' ppdA. dA5' ppdA is a better inhibitor of oligomer formation than N6,N6-dimethyladenine because both adenine rings of dA5' ppdA bind to the clay surface and block adjacent catalytic sites [15].
• An analysis of the effect of various purines and pyrimidines on the germination process in three different isolates of the late blight fungus, Phytophthora infestans, revealed increased rates of indirect germination in one isolate by adenine, hypoxanthine, and the riboside of dimethylaminopurine [16].

Gene context of NSC401568

• Dimethylaminopurine (DMAP) has previously been used as an inhibitor of phosphorylation in studies of meiotic events, and more recently to investigate TNFalpha signaling, because of its potential to inhibit activation of c-jun N-terminal kinase (JNK) [12].
• In addition, TNF-alpha-induced ROS production was inhibited by the acidic sphingomyelinase inhibitor desipramine (5 microM; -80%, n = 4, P < 0.01) and totally blocked by the ceramide-activated protein kinase (CAPK) inhibitor dimethylaminopurine (1 mM; n = 6, P < 0.05) [17].
• Modification of SPIN is inhibited by treatment with 6-dimethylaminopurine (DMAP), suggesting that SPIN is phosphorylated by a serine-threonine kinase [18].
• However, when pronuclear formation was advanced experimentally using 6-dimethylaminopurine (DMAP), concomitant advancement of surface uvomorulin was not observed [19].

Analytical, diagnostic and therapeutic context of NSC401568

• Karyotyping of 20 blastocysts and compact morula from each group determined that 65% of BL1 and 63% of DMAP embryos were diploid with the remainder mixoploid (2n + 4n) [20].

References