Disease relevance of Orientin

• CONCLUSIONS: Orientin and vicenin protect against foetal irradiation-induced genomic damage and instability, thereby reducing the delayed chromosomal abnormalities and tumorigenesis in adult [1].
• MATERIALS AND METHODS: Fourteen-day pregnant mice were exposed to 1 Gy 60Co gamma-radiation 30 min after an intraperitoneal injection of orientin or vicenin (50 microg kg(-1) body weight) [1].
• In previous studies, flavonoids, orientin and vicenin, that were isolated from the leaf extract of Ocimum sanctum, were found to protect mice against radiation injury [2].
• Vehicle or orientin (3, 10, 30 mumol l- 1) was added 10 min before hypoxia and reoxygenated [3].
• Orientin (0.5, 1.0 and 2.0 mg kg- 1) or its vehicle was injected i.v. 10 min prior to ischemia [3].

High impact information on Orientin

• The present finding that very low nontoxic doses of orientin and vicenin provide efficient protection against bone marrow damage at clinically relevant radiation doses suggests their potential for protection of normal tissues in radiotherapy [4].
• Protection of mouse bone marrow against radiation-induced chromosome damage and stem cell death by the ocimum flavonoids orientin and vicenin [4].
• To study bone marrow protection, adult Swiss mice were exposed to 0-6 Gy 60Co gamma rays 30 min after an intraperitoneal injection of 50 microg/ kg body weight of orientin/vicenin [4].
• Protection against prenatal irradiation-induced genomic instability and its consequences in adult mice by Ocimum flavonoids, orientin and vicenin [1].
• Radiation protection of human lymphocyte chromosomes in vitro by orientin and vicenin [5].

Biological context of Orientin

• Orientin and vicenin provided almost equal protection against radiation-induced lipid peroxidation in mouse liver [2].
• Radiation protection by the ocimum flavonoids orientin and vicenin: mechanisms of action [2].
• Dose modification factors (DMFs) for the LD50 were 1.37 for vicenin and 1.30 for orientin [6].
• Flow cytometric analysis also showed that apoptosis of cardiomyocytes was reduced by pretreatment with orientin (3, 10 and 30 mumol l- 1) [3].
• Vasodilatation produced by orientin and its mechanism study [7].

Anatomical context of Orientin

• Anti-apoptotic effect and the mechanism of orientin on ischaemic/reperfused myocardium [3].
• Orientin can stimulate NO production from endothelial cells [7].
• The results showed that Orientin relaxed thoracic aortic rings by the nitric oxide-cGMP pathway, and in the vascular smooth muscle inhibited the contraction induced by the activation of receptor-operating and voltage-dependent Ca2+ channels [7].
• Among the four compounds, studied, only Orientin relaxed phenylephrine-induced contractions with an IC50 value of 2.28 microM in the endothelium intact and with an IC50 value around 7.27 microM in the endothelium removed aortic rings [7].

Associations of Orientin with other chemical compounds

• Isoorientin and orientin presented similar activities toward the DPPH (IC(50): 9-10 microM) and inhibited lipid peroxidation by 70% at 100 microg/mL [8].
• Isoorientin, orientin, amentoflavone, leucocyanidol, eriodictyol, datiscetin and robinetin behaved as potent scavengers and structure-activity relationships were established [9].
• The main flavonoid constituents of the EtOAc extract, casticin, vitexin and orientin were assayed for antioxidant activity and found that only casticin possesses a marked lipid peroxidation inhibitory effect (IC(50) = 0.049 mm) compared with that of the positive control ascorbic acid (IC(50) = 0.703 mm) [10].
• The radioprotective effects of two flavonoids, orientin (Ot) and vicenin (Vc), obtained from the leaves of Ocimum sanctum, and the synthetic compounds WR-2721 and MPG (2-mercaptopropionyl glycine) have been compared by examining chromosome aberration in cells of bone marrow in irradiated mice [11].

Gene context of Orientin

• Therefore, the effect of orientin and vicenin on radiation-induced lipid peroxidation in vivo and their antioxidant activity in vitro were studied [2].

Analytical, diagnostic and therapeutic context of Orientin

• In addition, results of immunohistochemistry and Western blot analysis showed that orientin increased the expression of bcl-2 and reduced Bax expression, resulting in up-regulation of the bcl-2/Bax ratio [3].
• We investigated the anti-apoptotic effect of orientin, from bamboo leaves (Phyllostachys nigra), on rat heart after treatment with ischemia/reperfusion (I/R), and on rat cardiomyocytes injured by hypoxia/reoxygenation (H/R) [3].
• TUNEL assay and DNA fragmentation assay demonstrated that myocardium apoptosis was attenuated by pretreatment with orientin (0.5, 1.0 and 2.0 mg kg- 1) [3].

References