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Chemical Compound Review

NIGROSINE     disodium (3Z)-5-amino-6-(4...

Synonyms: Nigrosine B, Nigrosine WSB, HSDB 1971, Acid black 2, AC1NSE2P, ...
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Disease relevance of Amido black

  • Cytotoxicity was assessed by neutral red uptake and amido black staining tests; genotoxicity was assayed using the Ames test, Sister Chromatides Exchanges (SCE) and chromosomal aberrations [1].
  • A traditional electrophoretic procedure for detection of Bence-Jones proteinuria, employing Amido black stain on 200-fold concentrated urine, has been compared to two procedures employing highly sensitive protein stains not requiring prior urine concentration [2].
  • A PF patient's serum covalently coupled to a Staphylococcus aureus protein A column was incubated with the Con-A eluted products and the immuno-isolated antigen was separated by SDS-PAGE, transferred to a membrane, and visualized with Coomassie blue, silver and amido black stains [3].

High impact information on Amido black


Biological context of Amido black


Anatomical context of Amido black

  • A duplicate strip was stained with amido black or anti-myelin antibody to visualize the myelin proteins [14].
  • The amido black assay: a simple and quantitative multipurpose test of adhesion, proliferation, and cytotoxicity in microplate cultures of keratinocytes (HaCaT) and other cell types growing adherently or in suspension [15].
  • Staining of purified calf thymus histones H2A, H2B, H3, and H4 by this method is 30 times more sensitive than staining with amido black alone, allowing the detection of each histone and its modified forms down to the nanogram level [16].
  • Purified cell membrane preparations were run on gels and various protease activities were detected by amido black [17].
  • Sera from visceral yolk sac blood vessels, exocoelomic cavity fluid, and amniotic sac fluid were analyzed by SDS-polyacrylamide gel electrophoresis followed by Amido Black staining and fluorography [18].

Associations of Amido black with other chemical compounds


Gene context of Amido black


Analytical, diagnostic and therapeutic context of Amido black

  • Protein content of a wide variety of sample types may be determined by adsorption of microliter samples onto nitrocellulose, followed by amido black staining and laser densitometry [29].
  • The adsorption of albumin and fibrinogen to the surfaces was assessed using the Amido Black assay, whereas platelet attachment was studied by scanning electron microscopy and quantified using stereological techniques [30].
  • Castration for 14 days produced a sharp decrease in the colour intensity of bands B-E when stained with Amido black [31].
  • Here we have modified this 'blot and culture' technique by including Mr standard proteins in the same electrophoretic lane with the samples, identifying the proteins by staining the nitrocellulose blot with Amido black, marking the standard bands with pinholes and destaining the blot prior to seeding neurons onto it [32].
  • Almost all proteins stained by amido black were also revealed by Western blotting with antibodies to T. nattereri venom [33].


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  2. A comparison of three procedures for the detection of Bence-Jones proteinuria. Wong, W.K., Wieringa, G.E., Stec, Z., Russell, J., Cooke, S., Keevil, B.G., Lockhart, S. Ann. Clin. Biochem. (1997) [Pubmed]
  3. The tryptic cleavage product of the mature form of the bovine desmoglein 1 ectodomain is one of the antigen moieties immunoprecipitated by all sera from symptomatic patients affected by a new variant of endemic pemphigus. Abréu-Vélez, A.M., Javier Patiño, P., Montoya, F., Bollag, W.B. European journal of dermatology : EJD. (2003) [Pubmed]
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  6. Tandem scanning reflected-light microscopy of cell-substratum adhesions and stress fibres in Swiss 3T3 cells. Paddock, S.W. J. Cell. Sci. (1989) [Pubmed]
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  10. Cell adhesion to low-Mr proteins extractable from mineralized and soft connective tissues. Wong, H.J., Aubin, J.E., Wasi, S., Sodek, J. Biochem. J. (1985) [Pubmed]
  11. Histamine stimulates the proliferation of human articular chondrocytes in vitro and is expressed by chondrocytes in osteoarthritic cartilage. Tetlow, L.C., Woolley, D.E. Ann. Rheum. Dis. (2003) [Pubmed]
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  14. Activation of complement by myelin: identification of C1-binding proteins of human myelin from central nervous tissue. Vanguri, P., Shin, M.L. J. Neurochem. (1986) [Pubmed]
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  16. Silver staining of histones in Triton-acid-urea gels. Mold, D.E., Weingart, J., Assaraf, J., Lubahn, D.B., Kelner, D.N., Shaw, B.R., McCarty, K.S. Anal. Biochem. (1983) [Pubmed]
  17. Oncogene ras p21- and v-src pp60-transformed cells exhibit altered expression of proteases. DiStefano, J.F., Cotto, C.A., Lane, B., Hagag, N. Cancer Invest. (1988) [Pubmed]
  18. Uptake and distribution of exogenous serum proteins in the cultured rat embryo. Rhinehardt, A.E., Carey, S.W., Young, M.F., Klein, N.W. J. Exp. Zool. (1984) [Pubmed]
  19. Immunogenic proteins of Trichomonas vaginalis as demonstrated by the immunoblot technique. Garber, G.E., Proctor, E.M., Bowie, W.R. Infect. Immun. (1986) [Pubmed]
  20. Purification of outer membrane proteins of the gram-negative bacterium Neisseria gonorrhoeae. Judd, R.C. Anal. Biochem. (1988) [Pubmed]
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  22. Factors affecting the electrophoretic analysis of myelin proteins: application to changes occurring during brain development. Magno-Sumbilla, C., Campagnoni, A.T. Brain Res. (1977) [Pubmed]
  23. A modification of the tannic acid-phosphomolybdic acid-dye stain for demonstrating myoepithelial cells in formalin fixed tissue. Kellogg, A., McAuliffe, W.G., Schrodt, G.R. Stain technology. (1986) [Pubmed]
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  25. PRB3 null mutations result in absence of the proline-rich glycoprotein Gl and abolish Fusobacterium nucleatum interactions with saliva in vitro. Azen, E., Prakobphol, A., Fisher, S.J. Infect. Immun. (1993) [Pubmed]
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  27. Quantitation of fibroblast population growth rate in situ using computerized image analysis. Perricone, M.A., Saldate, V., Hyde, D.M. Microsc. Res. Tech. (1995) [Pubmed]
  28. Amido black 10 B-stained bovine serum albumin (BSA) in density gradient centrifugation. Kouvonen, I., Pettersson, K., Lövgren, T. Anal. Biochem. (1978) [Pubmed]
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  30. Protein adsorption and platelet attachment and activation, on TiN, TiC, and DLC coatings on titanium for cardiovascular applications. Jones, M.I., McColl, I.R., Grant, D.M., Parker, K.G., Parker, T.L. J. Biomed. Mater. Res. (2000) [Pubmed]
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  32. An examination of ciliary neuronotrophic factors from avian and rodent tissue extracts using a blot and culture technique. Rudge, J.S., Davis, G.E., Manthorpe, M., Varon, S. Brain Res. (1987) [Pubmed]
  33. Thalassophryne nattereri fish venom: biological and biochemical characterization and serum neutralization of its toxic activities. Lopes-Ferreira, M., Barbaro, K.C., Cardoso, D.F., Moura-Da-Silva, A.M., Mota, I. Toxicon (1998) [Pubmed]
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