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Chemical Compound Review

BIC3480     sodium 3-[[[4-[(Z)-[4-[(4...

Synonyms: AC1O3HAD, Acid Blue 90, FT-0623195, I14-19325, 6104-58-1, ...
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Disease relevance of Eriosin Brilliant Cyanine G


High impact information on Eriosin Brilliant Cyanine G

  • PAGE in the presence of 0.1% SDS demonstrates that striated flagellar roots are composed of a number of polypeptides, the most predominant one being a protein of 20,000 Mr. The 20,000 Mr protein band represents approximately 63% of the Coomassie Brilliant Blue staining of gels of isolated flagellar roots [6].
  • A silver/Coomassie brilliant blue R-250 staining technique that permits a color-coded differentiation of erythrocyte membrane proteins, sialoglycoproteins, and lipids in a single one-dimensional NaDodSO4/polyacrylamide gel has been described [7].
  • Polypeptides unique to the olfactory cilia preparation, compared to a control preparation of palate respiratory cilia, are identified by Coomassie brilliant blue staining, silver staining, and radiolabeled lectin overlays, as well as by biosynthetic labeling with 35S-methionine in epithelial explants and protein phosphorylation in isolated cilia [8].
  • The activator protein, exhaustively purified and designated as activator II in this study, showed a single protein band on sodium dodecyl sulfate-, native-, and isoelectrofocussing-polyacrylamide slab gel electrophoresis after being stained with Coomassie Brilliant Blue. Its molecular weight and pI were 69,200 and 4.0, respectively [9].
  • The strong staining of the TLX antigens with Coomassie Brilliant Blue and Amido Black indicates they are largely proteins [10].

Chemical compound and disease context of Eriosin Brilliant Cyanine G


Biological context of Eriosin Brilliant Cyanine G

  • Induction of the lambda OLPL promoter caused the appearance of L-fucose-H+ symport activity and of a Coomassie brilliant blue-stained 32 kDa membrane protein expressed at high levels sufficient for identification as FucP by N-terminal protein sequencing [14].
  • Reverse sister chromatid differential staining by Coomassie Brilliant blue R-250 [15].
  • After the protein spots had been electroblotted onto a polyvinylidene difluoride membrane and stained with Coomassie Brilliant Blue, the N-terminal amino acid sequences were determined for about twenty protein spots [16].
  • The comparison between such maps showed up- and downregulation of 45 polypeptide chains, of which 32 were downregulated and 13 upregulated, out of a total of 700 spots detected by a medium-sensitivity stain, micellar Coomassie Brilliant Blue. Fingerprinting by mass spectrometry analysis enabled the identification of 36 of these spots [17].
  • Enzymic deglycosylation of several chromatographic fractions of ribonuclease, which have different degrees of glycosylation, results in increased staining by Coomassie Brilliant Blue R-250 [18].

Anatomical context of Eriosin Brilliant Cyanine G


Associations of Eriosin Brilliant Cyanine G with other chemical compounds


Gene context of Eriosin Brilliant Cyanine G

  • The slower moving band, which probably corresponded to the higher polymeric form of the G6PD with high specific activity, was not seen on native PAGE due to insufficient protein for Coomassie brilliant blue staining [29].
  • When normal human serum and properdin were allowed to diffuse toward each other in agar gel for 16 hr, a line of precipitation could be seen when stained with Coomassie brilliant blue [30].
  • In the SDS-PAGE, one major band with a molecular weight of 56,000 and one minor band with 15,000 were stained with Coomassie Brilliant Blue and both showed DIF activity after extraction from gels [31].
  • KN-62 inhibited an increase in calcium entry induced by 4AP, while brilliant blue G (BBG) prevented it, supporting the hypothesis that purinergic P2X7 receptors are involved in this action [32].
  • The matrix metalloproteinase MMP13, a secretory protein, was expressed in MDCK cells at a level high enough to be detectable in crude supernatants without concentration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis stained with Coomassie brilliant blue R-250 [33].

Analytical, diagnostic and therapeutic context of Eriosin Brilliant Cyanine G


  1. Threshold of pathological proteinuria measured with Coomassie Brilliant Blue. Lievens, M.M., Ambroes, Y.I. Clin. Chem. (1985) [Pubmed]
  2. Identification of trichloroethanol visualized proteins from two-dimensional polyacrylamide gels by mass spectrometry. Ladner, C.L., Edwards, R.A., Schriemer, D.C., Turner, R.J. Anal. Chem. (2006) [Pubmed]
  3. Profiling of Caenorhabditis elegans proteins using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. Kaji, H., Tsuji, T., Mawuenyega, K.G., Wakamiya, A., Taoka, M., Isobe, T. Electrophoresis (2000) [Pubmed]
  4. P2X4 is Up-regulated in Gingival Fibroblasts after Periodontal Surgery. Binderman, I., Bahar, H., Jacob-Hirsch, J., Zeligson, S., Amariglio, N., Rechavi, G., Shoham, S., Yaffe, A. J. Dent. Res. (2007) [Pubmed]
  5. Coomassie brilliant blue G-250 dye-binding technique for determination of autolytic protein breakdown in Euglena gracilis and comparison to other methods of autolysis measurement. Krauspe, R., Scheer, A. Anal. Biochem. (1986) [Pubmed]
  6. Striated flagellar roots: isolation and partial characterization of a calcium-modulated contractile organelle. Salisbury, J.L., Baron, A., Surek, B., Melkonian, M. J. Cell Biol. (1984) [Pubmed]
  7. Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique. Dzandu, J.K., Deh, M.E., Barratt, D.L., Wise, G.E. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  8. Isolated frog olfactory cilia: a preparation of dendritic membranes from chemosensory neurons. Chen, Z., Pace, U., Heldman, J., Shapira, A., Lancet, D. J. Neurosci. (1986) [Pubmed]
  9. Activator proteins for glycosphingolipid hydrolysis by endoglycoceramidases. Elucidation of biological functions of cell-surface glycosphingolipids in situ by endoglycoceramidases made possible using these activator proteins. Ito, M., Ikegami, Y., Yamagata, T. J. Biol. Chem. (1991) [Pubmed]
  10. Isolation and identification of trophoblast lymphocyte cross-reactive (TLX) antigens from human lymphocytes. Kim, I.C. J. Biol. Chem. (1989) [Pubmed]
  11. Trypanosoma cruzi: polypeptide markers of epimastigotes and trypomastigotes. Rangel-Aldao, R., Comach, G., Allende, O., Cayama, E., Delgado, V., Piras, R., Piras, M., Henriquez, D., Negri, S. Mol. Biochem. Parasitol. (1986) [Pubmed]
  12. A technique for isolation of rubella virus-like particles by sucrose gradient ultracentrifugation using Coomassie brilliant blue G crystals. Giessauf, A., Flaim, M., Dierich, M.P., Würzner, R. Anal. Biochem. (2002) [Pubmed]
  13. Lipopolysaccharide interferes with the staining of lipoprotein on polyacrylamide gels. Loeb, M.R., Kilner, J. Biochim. Biophys. Acta (1978) [Pubmed]
  14. Identification of a novel sugar-H+ symport protein, FucP, for transport of L-fucose into Escherichia coli. Gunn, F.J., Tate, C.G., Henderson, P.J. Mol. Microbiol. (1994) [Pubmed]
  15. Reverse sister chromatid differential staining by Coomassie Brilliant blue R-250. Jan, K.Y. Exp. Cell Res. (1981) [Pubmed]
  16. Separation of heat-stable proteins from Thermus thermophilus HB8 by two-dimensional electrophoresis. Kawaguchi, S., Kuramitsu, S. Electrophoresis (1995) [Pubmed]
  17. Proteomic analysis of pancreatic ductal carcinoma cells treated with 5-aza-2'-deoxycytidine. Cecconi, D., Astner, H., Donadelli, M., Palmieri, M., Missiaglia, E., Hamdan, M., Scarpa, A., Righetti, P.G. Electrophoresis (2003) [Pubmed]
  18. Interference of the carbohydrate moiety in coomassie brilliant blue R-250 protein staining. Osset, M., Piñol, M., Fallon, M.J., de Llorens, R., Cuchillo, C.M. Electrophoresis (1989) [Pubmed]
  19. Annexin VI, a marker protein of hepatocytic endosomes. Jäckle, S., Beisiegel, U., Rinninger, F., Buck, F., Grigoleit, A., Block, A., Gröger, I., Greten, H., Windler, E. J. Biol. Chem. (1994) [Pubmed]
  20. Combined immunostaining and Coomassie Brilliant Blue staining of polyvinylidene difluoride membranes without organic solvent. Houen, G., Bruun, L., Barkholt, V. Electrophoresis (1997) [Pubmed]
  21. A continuous-flow technique with Coomassie Brilliant Blue for cerebrospinal fluid protein assay. Gillery, P., Locre, F., Malgras, A., Borel, J.P. Clin. Chem. (1985) [Pubmed]
  22. Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis. Bergqvist, Y., Karlsson, L., Fohlin, L. Clin. Chem. (1989) [Pubmed]
  23. Cell surface proteins of Candida albicans: preparation of extracts and improved detection of proteins. Vediyappan, G., Bikandi, J., Braley, R., Chaffin, W.L. Electrophoresis (2000) [Pubmed]
  24. Fluoride affects Coomassie Brilliant Blue measurement of protein in cerebrospinal fluid. Cheung, C.K., Chan, K.W., Chan, A.Y. Clin. Chem. (1990) [Pubmed]
  25. Quantifying proliferation of cultured human and rabbit airway smooth muscle cells in response to serum and platelet-derived growth factor. Hirst, S.J., Barnes, P.J., Twort, C.H. Am. J. Respir. Cell Mol. Biol. (1992) [Pubmed]
  26. Improving the detection of proteins after transfer to polyvinylidene difluoride membranes. Sanchez, J.C., Ravier, F., Pasquali, C., Frutiger, S., Paquet, N., Bjellqvist, B., Hochstrasser, D.F., Hughes, G.J. Electrophoresis (1992) [Pubmed]
  27. Comparison of different staining methods for polyvinylidene difluoride membranes. Christiansen, J., Houen, G. Electrophoresis (1992) [Pubmed]
  28. Improved detection of lymphocyte membrane proteins in purified form and as a crude mixture using native and denaturing polyacrylamide gel electrophoresis by optimisation of coomassie brilliant blue and silver staining. Warlow, R.S., Bernard, C.C. Electrophoresis (1990) [Pubmed]
  29. Purification and some properties of human placental glucose-6-phosphate dehydrogenase. Aksoy, Y., Ogüs, I.H., Oauzer, N. Protein Expr. Purif. (2001) [Pubmed]
  30. Complement activation in semi-solid medium: Insolubilization of properdin and the third component of complement (C3) in agar gels. Ziegler, J.B., Watson, L., Goodkofsky, I., Alper, C.A., Lepow, I.H. J. Immunol. (1976) [Pubmed]
  31. Purification and characterization of a T lymphocyte-derived differentiation-inducing factor for human promyelocytic cell line (HL-60) and its relationship to lymphotoxin. Shimizu, Y., Nakamura, T., Niki, T., Hemmi, H., Sugamura, K. Microbiol. Immunol. (1989) [Pubmed]
  32. 4-Aminopyridine activates calcium influx through modulation of the pore-forming purinergic receptor in human peripheral blood mononuclear cells. Lajdova, I., Chorvat, D., Spustova, V., Chorvatova, A. Can. J. Physiol. Pharmacol. (2004) [Pubmed]
  33. A high-level mammalian expression system based on the Madin-Darby canine kidney cell line. Pei, D., Yi, J. Protein Expr. Purif. (1998) [Pubmed]
  34. Impermeant maleimides. Oriented probes of erythrocyte membrane proteins. Abbott, R.E., Schachter, D. J. Biol. Chem. (1976) [Pubmed]
  35. Palmitylation of the glycoprotein IIb-IIIa complex in human blood platelets. Cierniewski, C.S., Krzeslowska, J., Pawlowska, Z., Witas, H., Meyer, M. J. Biol. Chem. (1989) [Pubmed]
  36. Characterization of allergens from spores of the oyster mushroom, Pleurotus ostreatus. Horner, W.E., Ibanez, M.D., Liengswangwong, V., Salvaggio, J.E., Lehrer, S.B. J. Allergy Clin. Immunol. (1988) [Pubmed]
  37. Identification of a major peanut allergen, Ara h I, in patients with atopic dermatitis and positive peanut challenges. Burks, A.W., Williams, L.W., Helm, R.M., Connaughton, C., Cockrell, G., O'Brien, T. J. Allergy Clin. Immunol. (1991) [Pubmed]
  38. Quantitative densitometry of proteins stained with coomassie blue using a Hewlett Packard scanjet scanner and Scanplot software. Vincent, S.G., Cunningham, P.R., Stephens, N.L., Halayko, A.J., Fisher, J.T. Electrophoresis (1997) [Pubmed]
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