Disease relevance of CCRIS 5906

• Diethanolamine (DEA) is a major industrial chemical which has low acute toxicity, but, on repeat exposure, has significant cumulative toxicity [1].
• On plates inoculated with Staphylococcus aureus ATCC 25923, CMW1 discs containing 2.5% by dry weight of cephalothin, coumermycin or fusidic acid (as diethanolamine fusidate) produced zones of inhibition for four to eight weeks when transferred daily [2].
• A 35-year-old man demonstrated sulfisoxazole diolamine-induced photosensitivity during treatment for staphylococcal blepharitis [3].
• Genetic alterations in the Catnb gene but not the H-ras gene in hepatocellular neoplasms and hepatoblastomas of B6C3F(1) mice following exposure to diethanolamine for 2 years [4].
• Diethanolamine is used as eluent in this single-step purification to prepare endotoxin-free pertussis toxin in good yield (70%) and high purity (greater than 95%) [5].

High impact information on CCRIS 5906

• This is followed by amination with diethanolamine [6].
• Linearity of precipitation was demonstrable up to a bone ALP activity (diethanolamine buffer 37 degrees C) of 2000 U/L, though a matrix effect was observed for dilutions of high-activity sera in saline or bovine serum albumin [7].
• We modified the Hybritech Tandem-E prostate-specific antigen (PSA) assay by increasing the sample volume, increasing enzyme-substrate incubation time, and using diethanolamine buffer [8].
• The antimicrobial effects of diethanolamine, dimethylamino-methyl-propanol, and butylethanolamine are greatly enhanced at high pH [9].
• Aminopropanediols and diethanolamine, also selectively inhibited ATPase activity of activated membranes after storage in the dark, whereas NH4Cl and imidazole inhibit the ATPase activity of activated thylakoids almost equally whether they are added directly after the illumination or several minutes later [10].

Biological context of CCRIS 5906

• Reproducibility of surface mu quantitation depends upon (1) maintaining cell viability during the reaction procedure, and (2) avoiding buffers containing diethanolamine for the alkaline phosphatase reaction [11].
• Gene expression analysis of mouse and rat hepatocytes following diethanolamine treatment showed increases in genes associated with cell growth and decreases in expression of genes involved in apoptotic pathways [12].
• Toxicology studies of diethanolamine were conducted in male and female B6C3F1 mice to characterize and compare effects of exposure in the drinking water with those caused by topical application and to compare responses in mice to those observed in rats [13].
• Significantly, the blood kinetics and ADME of TEA in mice and/or rats differs from that of a related chemical, diethanolamine, which appears to be more toxic to rodents than TEA [14].
• Investigations on cell proliferation in B6C3F(1) mouse liver by diethanolamine [15].

Anatomical context of CCRIS 5906

• In contrast, diethanolamine failed to increase DNA synthesis in human hepatocytes [12].
• Exposure to diethanolamine caused dose-dependent toxic effects in the liver (hepatocellular cytological alterations and necrosis), kidney (nephropathy and tubular epithelial necrosis in males), heart (cardiac myocyte degeneration) and skin (site of application: ulceration, inflammation, hyperkeratosis, and acanthosis) [13].
• Although oral administration was the preferred route, fusidic acid is not absorbed from the gastrointestinal tract of laboratory rats, and a subcutaneous injection of diethanolamine fusidate was used to achieve serum levels of the agent equivalent to those achieved clinically in man [16].
• Mucosal application of C. perfringens enterotoxin resulted in prompt increases in short-circuit current coupled with a reduction in transepithelial resistance consistent with movement of sodium and other cations smaller than diethanolamine from mucosa to serosa [17].
• Diethanolamine alters neurogenesis and induces apoptosis in fetal mouse hippocampus [18].

Associations of CCRIS 5906 with other chemical compounds

• The combination of 5-bromo-4-chloro-3-indolyl phosphate and nitroblue-tetrazolium salt in diethanolamine buffer at pH 9.6 was selected as buffer/substrate to yield stable, insoluble, and very intense purplish-blue precipitates on the surface of the cells labeled with monoclonal antibody-alkaline phosphatase conjugates [19].
• Choline supplementation inhibits diethanolamine-induced morphological transformation in syrian hamster embryo cells: evidence for a carcinogenic mechanism [20].
• Somatic mutations induced by maleic hydrazide and its potassium and diethanolamine salts in the Tradescantia mutation assay [21].
• Lauramide diethanolamine absorption, metabolism, and disposition in rats and mice after oral, intravenous, and dermal administration [22].
• METHODS: Levels of NO and oxygen free radicals were determined in both reoxygenation in vitro and reperfusion in vivo models using an NO electrochemical probe and high-performance liquid chromatography with the 2,3- and 2,5-dihydroxybenzoic acid trapping method, before and after addition of the NO donor diethanolamine nitric oxide (DEA/NO) [23].

Gene context of CCRIS 5906

• Control beads included those coated with diethanolamine (DEA), SAWA, or MBP [24].
• The present study characterized the immunohistochemical localization of beta-catenin protein in hepatocellular neoplasms and hepatoblastomas in B6C3F(1) mice exposed to diethanolamine (DEA) for 2 years and evaluated genetic alterations in the Catnb and H-ras genes which are known to play important roles in the pathogenesis of liver malignancies [4].
• This value is much higher than that for diethanolamine (228 kcal/mol) or for 18C6 (231 kcal/mol) [25].
• The aldehyde moiety of 1-hexadecanoyl-2-(5-oxopentanoyl)-GPC was found to react with FAB amine-containing liquid matrices (e.g., diethanolamine) to form an oxazolidine derivative (87 u increase in mass) [26].
• Differences between the six ALP isoforms with respect to diethanolamine buffer concentration and substrate pH were observed [27].

Analytical, diagnostic and therapeutic context of CCRIS 5906

• A simple and sensitive high performance liquid chromatographic (HPLC) method with UV detection was developed for the determination of new diethanolamine derivative complexing agents [28].
• Determination of diethanolamine or N-methyldiethanolamine in high ammonium concentration matrices by capillary electrophoresis with indirect UV detection: application to the analysis of refinery process waters [29].

References