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Gene Review

G3BP1  -  GTPase activating protein (SH3 domain)...

Homo sapiens

Synonyms: ATP-dependent DNA helicase VIII, G3BP, G3BP-1, GAP SH3 domain-binding protein 1, HDH-VIII, ...
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Disease relevance of G3BP1


High impact information on G3BP1

  • The RasGAP-associated endoribonuclease G3BP assembles stress granules [5].
  • In antigen-specific T cells, endogenous G3BP moved into a intracellular location adjacent to the immune synapse, but deeper inside the cell, upon antigen recognition [6].
  • Conversely, elimination of endogenous G3BP by RNA interference increased Lck Y505 phosphorylation and reduced TCR signaling [6].
  • As a consequence, G3BP augmented T-cell activation as measured by interleukin-2 gene activation [6].
  • A number of the genes assigned to the CDR represent good candidates for the 5q- syndrome, including MEGF1, G3BP, and several of the novel gene predictions [7].

Chemical compound and disease context of G3BP1

  • G3BP was also overexpressed in human breast tumors in parallel with HER2 overexpression and in an estrogen-independent manner, suggesting a role for G3BP in cancer progression [1].

Biological context of G3BP1


Anatomical context of G3BP1


Associations of G3BP1 with chemical compounds

  • Here, we show that G3BP, a phosphorylation-dependent endoribonuclease that interacts with RasGAP, is recruited to SGs in cells exposed to arsenite [5].
  • In quiescent cells, G3BP was hyperphosphorylated on serine residues, and this modification was essential for its activity [8].
  • Exogenous supplementation of glutathione inhibited SG formation elicited by arsenate or G3BP [13].
  • It should be emphasized that these results do not necessarily indicate a direct interaction of integrin with G3BP1 and rasGap120 [14].

Physical interactions of G3BP1


Regulatory relationships of G3BP1

  • Our results indicate that G3BP mRNA may be regulated by FMRP and supports the hypothesis that FMRP may modulate the transcription of specific transcripts [12].
  • Furthermore, recombinant G3BP and p137 expressed individually or together in mammalian or bacterial cells complemented the activity of the viral RNA polymerase and transcription factors [4].
  • We also show that G3BP promotes S phase entry in cultured fibroblasts deprived of serum and that this function is dependent on the presence of the RNA binding domain of the protein [17].

Other interactions of G3BP1

  • This interaction was further confirmed by performing a 2HS with G3BP, which led to the isolation of USP10 encoding cDNAs [15].
  • Microsequencing of the polypeptide showed that this enzyme corresponds to G3BP, an element of the Ras pathway which binds specifically to the GTPase-activating protein [18].
  • HDH VIII/G3BP is analogous to the heterogeneous nuclear ribonucleoproteins and contains a sequence rich in RGG boxes similar to the C-terminal domain of HDH IV/nucleolin, another DNA and RNA helicase [18].
  • Indeed, G3BP harbors a phosphorylation-dependent RNase activity which specifically cleaves the 3'-untranslated region of human c-myc mRNA [8].
  • In addition, we show a strong, negative effect on translation of the tau mRNA by IMP1, G3BP1, and HuD proteins in HEK-293 cells [11].

Analytical, diagnostic and therapeutic context of G3BP1


  1. Heregulin induces expression, ATPase activity, and nuclear localization of G3BP, a Ras signaling component, in human breast tumors. Barnes, C.J., Li, F., Mandal, M., Yang, Z., Sahin, A.A., Kumar, R. Cancer Res. (2002) [Pubmed]
  2. Galectin 3 and its binding protein in rheumatoid arthritis. Ohshima, S., Kuchen, S., Seemayer, C.A., Kyburz, D., Hirt, A., Klinzing, S., Michel, B.A., Gay, R.E., Liu, F.T., Gay, S., Neidhart, M. Arthritis Rheum. (2003) [Pubmed]
  3. Identification of metastasis associated gene G3BP by differential display in human cancer cell sublines with different metastatic potentials G3BP as highly expressed in non-metastatic. Liu, Y., Zheng, J., Fang, W., You, J., Wang, J., Cui, X., Wu, B. Chin. Med. J. (2001) [Pubmed]
  4. Vaccinia virus intermediate stage transcription is complemented by Ras-GTPase-activating protein SH3 domain-binding protein (G3BP) and cytoplasmic activation/proliferation-associated protein (p137) individually or as a heterodimer. Katsafanas, G.C., Moss, B. J. Biol. Chem. (2004) [Pubmed]
  5. The RasGAP-associated endoribonuclease G3BP assembles stress granules. Tourrière, H., Chebli, K., Zekri, L., Courselaud, B., Blanchard, J.M., Bertrand, E., Tazi, J. J. Cell Biol. (2003) [Pubmed]
  6. Removal of C-terminal SRC kinase from the immune synapse by a new binding protein. Rahmouni, S., Vang, T., Alonso, A., Williams, S., van Stipdonk, M., Soncini, C., Moutschen, M., Schoenberger, S.P., Mustelin, T. Mol. Cell. Biol. (2005) [Pubmed]
  7. Narrowing and genomic annotation of the commonly deleted region of the 5q- syndrome. Boultwood, J., Fidler, C., Strickson, A.J., Watkins, F., Gama, S., Kearney, L., Tosi, S., Kasprzyk, A., Cheng, J.F., Jaju, R.J., Wainscoat, J.S. Blood (2002) [Pubmed]
  8. A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein: a potential link between signal transduction and RNA stability. Gallouzi, I.E., Parker, F., Chebli, K., Maurier, F., Labourier, E., Barlat, I., Capony, J.P., Tocque, B., Tazi, J. Mol. Cell. Biol. (1998) [Pubmed]
  9. Tracking and elucidating alphavirus-host protein interactions. Cristea, I.M., Carroll, J.W., Rout, M.P., Rice, C.M., Chait, B.T., Macdonald, M.R. J. Biol. Chem. (2006) [Pubmed]
  10. Characterization of G3BPs: tissue specific expression, chromosomal localisation and rasGAP(120) binding studies. Kennedy, D., French, J., Guitard, E., Ru, K., Tocque, B., Mattick, J. J. Cell. Biochem. (2001) [Pubmed]
  11. Dynamic association with polysomes during P19 neuronal differentiation and an untranslated-region-dependent translation regulation of the tau mRNA by the tau mRNA-associated proteins IMP1, HuD, and G3BP1. Atlas, R., Behar, L., Sapoznik, S., Ginzburg, I. J. Neurosci. Res. (2007) [Pubmed]
  12. Reduced mRNA for G3BP in fragile X cells: evidence of FMR1 gene regulation. Zhong, N., Ju, W., Nelson, D., Dobkin, C., Brown, W.T. Am. J. Med. Genet. (1999) [Pubmed]
  13. Regulation of cytoplasmic stress granules by apoptosis-inducing factor. Candé, C., Vahsen, N., Métivier, D., Tourrière, H., Chebli, K., Garrido, C., Tazi, J., Kroemer, G. J. Cell. Sci. (2004) [Pubmed]
  14. Association of rasGAPSH3 binding protein 1, G3BP1, and rasGap120 with integrin containing complexes induced by an adhesion blocking antibody. Meng, X., Krishnan, J., She, Y., Ens, W., Standing, K., Wilkins, J.A. J. Proteome Res. (2004) [Pubmed]
  15. Ras-GAP SH3 domain binding protein (G3BP) is a modulator of USP10, a novel human ubiquitin specific protease. Soncini, C., Berdo, I., Draetta, G. Oncogene (2001) [Pubmed]
  16. Upregulation of the RAS-GTPase activating protein (GAP)-binding protein (G3BP) in proliferating RPE cells. Kociok, N., Esser, P., Unfried, K., Parker, F., Schraermeyer, U., Grisanti, S., Toqué, B., Heimann, K. J. Cell. Biochem. (1999) [Pubmed]
  17. G3BP is overexpressed in human tumors and promotes S phase entry. Guitard, E., Parker, F., Millon, R., Abecassis, J., Tocqué, B. Cancer Lett. (2001) [Pubmed]
  18. Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the ras transduction pathway. Costa, M., Ochem, A., Staub, A., Falaschi, A. Nucleic Acids Res. (1999) [Pubmed]
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