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Gene Review

Acsl1  -  acyl-CoA synthetase long-chain family...

Mus musculus

Synonyms: Acas, Acas1, Acs, Acsl2, FACS, ...
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Disease relevance of Acsl1

  • Lymphoma cell variants were selected on the FACS for differences in LPAM-1 expression: the binding capacity of these variants to Peyer's patch HEV directly correlates with the level of LPAM-1 expression [1].
  • The successful birth of offspring from recipient mice without transmission of leukemia to the recipients indicates the potential of autotransplantation of germ cells sorted by FACS to treat infertility secondary to anticancer treatment for childhood leukemia [2].
  • To test the hypothesis that mismatch between myocardial fatty acid uptake and utilization leads to the accumulation of cardiotoxic lipid species, and to establish a mouse model of metabolic cardiomyopathy, we generated transgenic mouse lines that overexpress long-chain acyl-CoA synthetase in the heart (MHC-ACS) [3].
  • Molecular analysis of NOD/SCID BM confirmed the high levels of engraftment of human transduced cells deduced from FACS analysis [4].
  • Also, granuloma T cells were isolated by FACS [5].

Psychiatry related information on Acsl1


High impact information on Acsl1

  • ALCAM+ perichondrial cells isolated by FACS exhibit the characteristics of mesenchymal stem cells [7].
  • Second, FACS analyses showed that an Ag-Ab complex is preferentially targeted by SpA to a subpopulation of splenocytes mainly composed of B cells [8].
  • Northern blot and FACS analyses suggest that the hCD40-L is restricted in its expression to T lymphocytes, and that it is most abundant on the CD4+ T cell subpopulation [9].
  • This was shown by FACS analysis as this putative pathogenic population was diminished in both spleen and lymph node [10].
  • To seek information on the capacity of mature T cells to migrate to the thymus, mice were injected with Thy-1-marked populations enriched for resting T cells or T blast cells; localization of the donor cells in the host thymus was assessed by staining cryostat sections of thymus and by FACS analysis of cell suspensions [11].

Chemical compound and disease context of Acsl1

  • We have used 3 color FACS analysis (together with dead cell exclusion by propidium iodide) to ascertain the levels of lymphoid lineage cells present in typical young adult (2-5 month) SCID mice [12].
  • FACS analysis showed a pronounced increase in intracellular adriamycin accumulation in the presence of hyperthermia [13].

Biological context of Acsl1

  • Long-chain fatty acyl-CoA synthetase (FACS) catalyzes esterification of long-chain fatty acids (LCFAs) with coenzyme A (CoA), the first step in fatty acid metabolism [14].
  • The phenotype of migratory cells was then examined by two-color immunocytochemistry and FACS analysis [15].
  • We have studied a panel of DBA/2 T cell clones specific for sperm whale myoglobin (SpW Mb) for TCR (T cell receptor) beta chain gene expression by FACS analysis using the monoclonal antibodies F23.1 and KJ16 specific for the V beta 8 family of the TCR beta chain genes [16].
  • Furthermore, Ly-1 B cells, enriched by a FACS, were shown to integrate the defective genome and appeared to be a major virus-infected B cell population [17].
  • However, using sensitive techniques such as flow cytometry (FACS) and cell-surface-specific biotinylation, we now show that there is significant surface expression of these proteins [18].

Anatomical context of Acsl1

  • Subcellular fractionation of adipocytes by differential density centrifugation reveals immunoreactive and enzymatically active FACS in several membrane fractions, including the plasma membrane [14].
  • To explore how FACS contributes to LCFA import, we examined the subcellular location of this enzyme in 3T3-L1 adipocytes which natively express this protein and which efficiently take up LCFAs [14].
  • Multicolor FACS analyses of CD4+ T cell subsets showed that development of phenotypic abnormalities of these cells at 9 wk after infection was completely inhibited by mu-suppression [19].
  • Double-color fluorescence experiments and FACS analysis showed that BB3+ cells were restricted to the CD3+ fraction of peripheral blood lymphocytes; in addition, in several donors the percentages (0.5-8% of total PBL) of BB3+ cells paralleled those of CD3+ WT31- cells [20].
  • Two-color FACS analysis of mouse bone marrow reveals a rare population, comprising 0.1-0.3% of the total, that expresses low levels of the Thy-1 antigen but does not express any of five surface markers that characterize differentiated hematolymphoid cells [21].

Associations of Acsl1 with chemical compounds

  • The very long chain acyl-CoA synthetase activity of the two enzymes was comparable as were the Km values for both ATP and coenzyme A. Interestingly, FATP1 was insensitive to inhibition by triacsin C, whereas ACS1 was inhibited by micromolar concentrations of the compound [22].
  • Low density, lineage-negative (CD4-, CD8-, B220-, Mac-1-, Gr-1-), CD71-negative, class I highly positive, FACS-sorted cells from 5-FU-treated C57BL/6 (B6) donor mice were transplanted into lethally irradiated BALB/c recipients [23].
  • FACS analysis of cell surface oligosaccharides using either Concanavalin A or L-phytohemagglutinin lectins confirmed an increase in high mannose groups and a decrease in tri- and tetra antennary beta-linked galactose-N-acetylglucosamine on mannose residues of Asn-linked oligosaccharides upon drug treatment [24].
  • However, cell cycle analysis of these two fractions by FACS using propidium iodide showed a similar pattern of cell cycle stages in both low and high density populations (G0 + G1 approximately 96 to 98% and S/G2 + M approximately 2 to 4%) [25].
  • FACS analysis and immunohistochemical studies with DQ beta-specific mAb demonstrated that DQ beta molecules in association with mouse A alpha f molecules are expressed on peripheral mononuclear cells, spleen cells, and in thymic medulla [26].

Other interactions of Acsl1


Analytical, diagnostic and therapeutic context of Acsl1


  1. Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha. Holzmann, B., McIntyre, B.W., Weissman, I.L. Cell (1989) [Pubmed]
  2. Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia. Fujita, K., Ohta, H., Tsujimura, A., Takao, T., Miyagawa, Y., Takada, S., Matsumiya, K., Wakayama, T., Okuyama, A. J. Clin. Invest. (2005) [Pubmed]
  3. A novel mouse model of lipotoxic cardiomyopathy. Chiu, H.C., Kovacs, A., Ford, D.A., Hsu, F.F., Garcia, R., Herrero, P., Saffitz, J.E., Schaffer, J.E. J. Clin. Invest. (2001) [Pubmed]
  4. Efficient transduction of human hematopoietic repopulating cells generating stable engraftment of transgene-expressing cells in NOD/SCID mice. Barquinero, J., Segovia, J.C., Ramírez, M., Limón, A., Güenechea, G., Puig, T., Briones, J., García, J., Bueren, J.A. Blood (2000) [Pubmed]
  5. IL-2 contributes to the IL-5 response in granulomas from mice infected with Schistosoma mansoni. Metwali, A., Elliott, D., Mathew, R., Blum, A., Weinstock, J.V. J. Immunol. (1993) [Pubmed]
  6. All T15 Id-positive antibodies (but not the majority of VHT15+ antibodies) are produced by peritoneal CD5+ B lymphocytes. Masmoudi, H., Mota-Santos, T., Huetz, F., Coutinho, A., Cazenave, P.A. Int. Immunol. (1990) [Pubmed]
  7. Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation. Arai, F., Ohneda, O., Miyamoto, T., Zhang, X.Q., Suda, T. J. Exp. Med. (2002) [Pubmed]
  8. Presentation of antigen in immune complexes is boosted by soluble bacterial immunoglobulin binding proteins. Léonetti, M., Galon, J., Thai, R., Sautès-Fridman, C., Moine, G., Ménez, A. J. Exp. Med. (1999) [Pubmed]
  9. Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion. Spriggs, M.K., Armitage, R.J., Strockbine, L., Clifford, K.N., Macduff, B.M., Sato, T.A., Maliszewski, C.R., Fanslow, W.C. J. Exp. Med. (1992) [Pubmed]
  10. Reduction of lupus nephritis in MRL/lpr mice by a bacterial superantigen treatment. Kim, C., Siminovitch, K.A., Ochi, A. J. Exp. Med. (1991) [Pubmed]
  11. Reentry of T cells to the adult thymus is restricted to activated T cells. Agus, D.B., Surh, C.D., Sprent, J. J. Exp. Med. (1991) [Pubmed]
  12. Analysis of lymphoid population in scid mice; detection of a potential B lymphocyte progenitor population present at normal levels in scid mice by three color flow cytometry with B220 and S7. Hardy, R.R., Kemp, J.D., Hayakawa, K. Curr. Top. Microbiol. Immunol. (1989) [Pubmed]
  13. Augmentative effects of intracellular chemotherapy penetration combined with hyperthermia in human ovarian cancer cells lines. Maymon, R., Bar-Shira Maymon, B., Holzinger, M., Tartakovsky, B., Leibovici, J. Gynecol. Oncol. (1994) [Pubmed]
  14. Localization of adipocyte long-chain fatty acyl-CoA synthetase at the plasma membrane. Gargiulo, C.E., Stuhlsatz-Krouper, S.M., Schaffer, J.E. J. Lipid Res. (1999) [Pubmed]
  15. Migration and maturation of Langerhans cells in skin transplants and explants. Larsen, C.P., Steinman, R.M., Witmer-Pack, M., Hankins, D.F., Morris, P.J., Austyn, J.M. J. Exp. Med. (1990) [Pubmed]
  16. Correlation of T cell receptor V beta gene family with MHC restriction. Morel, P.A., Livingstone, A.M., Fathman, C.G. J. Exp. Med. (1987) [Pubmed]
  17. Delayed progression of a murine retrovirus-induced acquired immunodeficiency syndrome in X-linked immunodeficient mice. Hitoshi, Y., Okada, Y., Sonoda, E., Tominaga, A., Makino, M., Suzuki, K., Kinoshita, J., Komuro, K., Mizuochi, T., Takatsu, K. J. Exp. Med. (1993) [Pubmed]
  18. Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein. Ramprasad, M.P., Terpstra, V., Kondratenko, N., Quehenberger, O., Steinberg, D. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  19. B cells are required for induction of T cell abnormalities in a murine retrovirus-induced immunodeficiency syndrome. Cerny, A., Hügin, A.W., Hardy, R.R., Hayakawa, K., Zinkernagel, R.M., Makino, M., Morse, H.C. J. Exp. Med. (1990) [Pubmed]
  20. A monoclonal antibody specific for a common determinant of the human T cell receptor gamma/delta directly activates CD3+WT31- lymphocytes to express their functional program(s). Ciccone, E., Ferrini, S., Bottino, C., Viale, O., Prigione, I., Pantaleo, G., Tambussi, G., Moretta, A., Moretta, L. J. Exp. Med. (1988) [Pubmed]
  21. Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus. Spangrude, G.J., Muller-Sieburg, C.E., Heimfeld, S., Weissman, I.L. J. Exp. Med. (1988) [Pubmed]
  22. Characterization of the Acyl-CoA synthetase activity of purified murine fatty acid transport protein 1. Hall, A.M., Smith, A.J., Bernlohr, D.A. J. Biol. Chem. (2003) [Pubmed]
  23. Purified hematopoietic stem cells without facilitating cells can repopulate fully allogeneic recipients across entire major histocompatibility complex transplantation barrier in mice. Wang, B., El-Badri, N.S., Cherry, n.u.l.l., Good, R.A. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  24. The alpha-glucosidase I inhibitor castanospermine alters endothelial cell glycosylation, prevents angiogenesis, and inhibits tumor growth. Pili, R., Chang, J., Partis, R.A., Mueller, R.A., Chrest, F.J., Passaniti, A. Cancer Res. (1995) [Pubmed]
  25. Cytokine synthesis by intestinal intraepithelial lymphocytes. Both gamma/delta T cell receptor-positive and alpha/beta T cell receptor-positive T cells in the G1 phase of cell cycle produce IFN-gamma and IL-5. Yamamoto, M., Fujihashi, K., Beagley, K.W., McGhee, J.R., Kiyono, H. J. Immunol. (1993) [Pubmed]
  26. Thymic deletion of V beta 11+, V beta 5+ T cells in H-2E negative, HLA-DQ beta+ single transgenic mice. Zhou, P., Anderson, G.D., Savarirayan, S., Inoko, H., David, C.S. J. Immunol. (1991) [Pubmed]
  27. Expression and regulation of mRNA for putative fatty acid transport related proteins and fatty acyl CoA synthase in murine epidermis and cultured human keratinocytes. Harris, I.R., Farrell, A.M., Memon, R.A., Grunfeld, C., Elias, P.M., Feingold, K.R. J. Invest. Dermatol. (1998) [Pubmed]
  28. Mouse very long-chain acyl-CoA synthetase in X-linked adrenoleukodystrophy. Heinzer, A.K., Kemp, S., Lu, J.F., Watkins, P.A., Smith, K.D. J. Biol. Chem. (2002) [Pubmed]
  29. Murine bubblegum orthologue is a microsomal very long-chain acyl-CoA synthetase. Fraisl, P., Forss-Petter, S., Zigman, M., Berger, J. Biochem. J. (2004) [Pubmed]
  30. Changes in hematopoiesis-supporting ability of C3H10T1/2 mouse embryo fibroblasts during differentiation. Nishikawa, M., Ozawa, K., Tojo, A., Yoshikubo, T., Okano, A., Tani, K., Ikebuchi, K., Nakauchi, H., Asano, S. Blood (1993) [Pubmed]
  31. DNA microarray gene expression profile of T cells with the splice variants of TCRzeta mRNA observed in systemic lupus erythematosus. Tsuzaka, K., Nozaki, K., Kumazawa, C., Shiraishi, K., Setoyama, Y., Yoshimoto, K., Suzuki, K., Abe, T., Takeuchi, T. J. Immunol. (2006) [Pubmed]
  32. Participation of bone marrow-derived cells in fibrotic changes in denervated skeletal muscle. Mochizuki, Y., Ojima, K., Uezumi, A., Masuda, S., Yoshimura, K., Takeda, S. Am. J. Pathol. (2005) [Pubmed]
  33. Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells. Gorczynski, L., Chen, Z., Hu, J., Kai, Y., Lei, J., Ramakrishna, V., Gorczynski, R.M. J. Immunol. (1999) [Pubmed]
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