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Gene Review

Itga5  -  integrin alpha 5 (fibronectin receptor alpha)

Mus musculus

Synonyms: CD49 antigen-like family member E, Cd49e, Fibronectin receptor subunit alpha, Fnra, Integrin alpha-5, ...
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Disease relevance of Itga5

  • Analysis of levels of expression comparing resting peritoneal macrophages with macrophages elicited using inflammatory stimuli indicated that alpha chain message and surface VLA-5 expression were significantly increased using thioglycollate or Listeria monocytogenes as stimuli to elicit cells [1].
  • Requirement for VLA-4 and VLA-5 integrins in lymphoma cells binding to and migration beneath stromal cells in culture [2].
  • The adhesion of anti-CD3-stimulated T cells to syngeneic MCA-38 mouse colon adenocarcinoma cells did not involve LFA-1 (alpha(L)beta(2)) or VLA-5 (alpha(5)beta(1)) [3].
  • Consequently, the CH-296 fragment can enhance GT through binding to both retrovirus particles and target cells that express integrins VLA-5 and/or VLA-4 [4].
  • These results strongly suggest that intrahepatic metastasis of CBO140C12 tumors is partly due to the marked invasive and adhesive abilities of tumor cells mediated by expression of MMP-9 and integrin alpha3beta1 (VLA-3), integrin alpha5beta1 (VLA-5) on the tumor surface, respectively [5].

High impact information on Itga5

  • Moreover, macrophage clearance into lymphatics can be blocked in vivo by RGD peptides and VLA-4 and VLA-5 but not beta(2) blocking antibodies [6].
  • We observed that macrophage-mesothelial adhesion is Arg-Gly-Asp (RGD) sensitive and partially mediated by very late antigen (VLA)-4 and VLA-5 but not alpha(v) or beta(2) integrins [6].
  • These results indicate that macrophage VLA-5 expression can be modulated in vivo and may provide an important mechanism by which macrophages are recruited to or adhere to fibronectin in inflammatory foci [1].
  • Molecular cloning of a murine fibronectin receptor and its expression during inflammation. Expression of VLA-5 is increased in activated peritoneal macrophages in a manner discordant from major histocompatibility complex class II [1].
  • Human fibronectin receptor (VLA-5) alpha and beta chain probes were used to identify their mouse homologues in a thioglycollate-elicited peritoneal exudate cell cDNA library [1].

Biological context of Itga5


Anatomical context of Itga5

  • Here we show that the expression of VLA-5 is restricted to thioglycolate-induced inflammatory macrophages and is not found in the resident macrophages [9].
  • Finally, Tpo stimulated adhesion of primitive (CD34+ CD38-) human bone marrow cells to fibronectin, predominantly through activation of VLA-5, whereas no such effect could be observed on CD34+ CD38+ bone marrow cells [12].
  • However, this cell line did not express classical FN receptors such as very late antigen (VLA)-4 and VLA-5, as estimated by immunofluorescent staining [13].
  • Immunohistochemical studies revealed that the integrins VLA 3, VLA 5 and integrin receptors to vitronectin are expressed in the epithelium and/or mesenchyme during the glandular stage of murine lung development [14].
  • We conclude that CALP1 and 2 can inhibit VLA-5-mediated adhesion of mast cells to fibronectin through binding to EF-hands of multiple proteins, and that these peptides can be used as lead compounds for the development of future therapy against allergy [10].

Associations of Itga5 with chemical compounds

  • Furthermore, the enhancing effect by FN was inhibited in the presence of the Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) peptide but not in the presence of the connecting segment-1 (CS-1) peptide, suggesting that enhancement of AICD observed in CD4+ CD8+ thymocytes is mediated through VLA-5 [15].
  • Our results suggest a novel binding pathway, in which the VLA-5 integrin binds to L1 on adjacent cells [16].
  • No inhibition was found with antibodies against glycoprotein IIb:IIIa, against the alpha 2 chain of VLA-2, and against the alpha 5 chain of VLA-5 [17].
  • Ligand-binding affinity of VLA-5 was also augmented by receptor tyrosine kinases when the phospholipase Cgamma-1/protein kinase C pathway was inhibited [18].
  • Expression of very late antigen (VLA)-4, VLA-5, and CXCR4 of CD133(+) cells was not modified by imatinib, but imatinib decreased the ability of CD133(+) cells to migrate [19].

Physical interactions of Itga5

  • Previous studies have shown the presence of several cation-binding domains in VLA-5 that are homologous to the calcium-binding EF-hands of calmodulin [10].

Regulatory relationships of Itga5

  • They adhered to fibronectin through VLA-5 when stimulated with steel factor and also directly to membrane-anchored steel factor through c-kit [20].
  • The IEC adhesion to FN was inhibited by specific antibody against the FN receptor (VLA-5), as well as competitive Arg-Gly-Asp (RGD) peptide [21].
  • CFTL-15 cells were found to express both VLA-4 and -5 integrins whereas MC/9 cells expressed only VLA-5 but not VLA-4 [22].
  • In monocytic ESb-MP cells the molecule serves as a ligand for P-selectin and triggering with CD24 specific antibodies can activate VLA-5/L1-mediated cell adhesion in these cells [23].

Other interactions of Itga5

  • Herein, we investigated the expression of receptors for fibronectin [very late antigen (VLA)-4 and VLA-5] and laminin (VLA-6), known to play a role in thymocyte migration [24].
  • VLA-4 was predominantly expressed at the CD4- CD8- stage, and thereafter the expression was reduced, whereas VLA-5 was constantly expressed during maturation [15].
  • This adhesion occurs to the same extent as that triggered by optimal levels of Steel factor (SF) or IgE + antigen (IgE + Ag) and is mediated by an increased avidity of the integrin very late antigen 5 (VLA-5) [25].
  • Interestingly only CALP2 was able to inhibit Mn(2+)-induced calmodulin-independent adhesion by interfering with an extracellular target, which is probably VLA-5 [10].
  • In this study, we report the preparation of a new monoclonal antibody (mAb) against mouse VLA-5 (alpha 5 beta 1) integrin [22].

Analytical, diagnostic and therapeutic context of Itga5


  1. Molecular cloning of a murine fibronectin receptor and its expression during inflammation. Expression of VLA-5 is increased in activated peritoneal macrophages in a manner discordant from major histocompatibility complex class II. Holers, V.M., Ruff, T.G., Parks, D.L., McDonald, J.A., Ballard, L.L., Brown, E.J. J. Exp. Med. (1989) [Pubmed]
  2. Requirement for VLA-4 and VLA-5 integrins in lymphoma cells binding to and migration beneath stromal cells in culture. Miyake, K., Hasunuma, Y., Yagita, H., Kimoto, M. J. Cell Biol. (1992) [Pubmed]
  3. Adenosine suppresses alpha(4)beta(7) integrin-mediated adhesion of T lymphocytes to colon adenocarcinoma cells. MacKenzie, W.M., Hoskin, D.W., Blay, J. Exp. Cell Res. (2002) [Pubmed]
  4. Enhancement of retroviral gene transduction on a dish coated with a cocktail of two different polypeptides: one exhibiting binding activity toward target cells, and the other toward retroviral vectors. Asada, K., Uemori, T., Ueno, T., Hashino, K., Koyama, N., Kawamura, A., Kato, I. J. Biochem. (1998) [Pubmed]
  5. A new pseudo-peptide of Arg-Gly-Asp (RGD) inhibits intrahepatic metastasis of orthotopically implanted murine hepatocellular carcinoma. Tsuchiya, Y., Sawada, S., Tsukada, K., Saiki, I. Int. J. Oncol. (2002) [Pubmed]
  6. Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. Bellingan, G.J., Xu, P., Cooksley, H., Cauldwell, H., Shock, A., Bottoms, S., Haslett, C., Mutsaers, S.E., Laurent, G.J. J. Exp. Med. (2002) [Pubmed]
  7. The fibronectin receptor, alpha subunit (Itga5) maps to murine chromosome 15, distal to D15Mit16. Adkison, L.R., White, R.A., Haney, D.M., Lee, J.C., Pusey, K.T., Gardner, J. Mamm. Genome (1994) [Pubmed]
  8. Potent cyclic peptide inhibitors of VLA-4 (alpha4beta1 integrin)-mediated cell adhesion. Discovery of compounds like cyclo(MePhe-Leu-Asp-Val-D-Arg-D-Arg) (ZD7349) compatible with depot formulation. Dutta, A.S., Gormley, J.J., Coath, M., Hassall, L., Hayward, C.F., Gellert, P.R., Kittlety, R.S., Alcock, P.J., Ferguson, R., Halterman, T., Jamieson, A., Moors, J.A., Moores, J.M., Rees, A., Wood, L.J., Reilly, C.F., Haworth, D. J. Pept. Sci. (2000) [Pubmed]
  9. Expression and Distribution of Very Late Antigen-5 in Mouse Peritoneal Macrophages upon Ingestion of Fibronectin-Bound Staphylococcus aureus. Shinji, H., Kamada, M., Seki, K., Tajima, A., Iwase, T., Masuda, S. Microbiol. Immunol. (2007) [Pubmed]
  10. Attenuation of very late antigen-5-mediated adhesion of bone marrow-derived mast cells to fibronectin by peptides with inverted hydropathy to EF-hands. Houtman, R., Ten Broeke, R., Blalock, J.E., Villain, M., Koster, A.S., Nijkamp, F.P. J. Immunol. (2001) [Pubmed]
  11. Potent cyclic monomeric and dimeric peptide inhibitors of VLA-4 (alpha4beta1 integrin)-mediated cell adhesion based on the Ile-Leu-Asp-Val tetrapeptide. Dutta, A.S., Crowther, M., Gormley, J.J., Hassall, L., Hayward, C.F., Gellert, P.R., Kittlety, R.S., Alcock, P.J., Jamieson, A., Moores, J.M., Rees, A., Wood, L.J., Reilly, C.F., Haworth, D. J. Pept. Sci. (2000) [Pubmed]
  12. Thrombopoietin promotes adhesion of primitive human hemopoietic cells to fibronectin and vascular cell adhesion molecule-1: role of activation of very late antigen (VLA)-4 and VLA-5. Cui, L., Ramsfjell, V., Borge, O.J., Veiby, O.P., Lok, S., Jacobsen, S.E. J. Immunol. (1997) [Pubmed]
  13. Use of a novel fibronectin receptor for liver infiltration by a mouse lymphoma cell line RL-male1. Gazi, M.H., Ito, M. Cancer Res. (1999) [Pubmed]
  14. Potential role of RGD-binding integrins in mammalian lung branching morphogenesis. Roman, J., Little, C.W., McDonald, J.A. Development (1991) [Pubmed]
  15. Enhancement of activation-induced cell death by fibronectin in murine CD4+ CD8+ thymocytes. Takayama, E., Kina, T., Katsura, Y., Tadakuma, T. Immunology (1998) [Pubmed]
  16. The L1 adhesion molecule is a cellular ligand for VLA-5. Ruppert, M., Aigner, S., Hubbe, M., Yagita, H., Altevogt, P. J. Cell Biol. (1995) [Pubmed]
  17. Platelet adhesion to laminin: role of Ca2+ and Mg2+ ions, shear rate, and platelet membrane glycoproteins. Hindriks, G., Ijsseldijk, M.J., Sonnenberg, A., Sixma, J.J., de Groot, P.G. Blood (1992) [Pubmed]
  18. Affinity modulation of very late antigen-5 through phosphatidylinositol 3-kinase in mast cells. Kinashi, T., Asaoka, T., Setoguchi, R., Takatsu, K. J. Immunol. (1999) [Pubmed]
  19. Despite inhibition of hematopoietic progenitor cell growth in vitro, the tyrosine kinase inhibitor imatinib does not impair engraftment of human CD133+ cells into NOD/SCIDbeta2mNull mice. Pirson, L., Baron, F., Meuris, N., Giet, O., Castermans, E., Greimers, R., Di Stefano, I., Gothot, A., Beguin, Y. Stem Cells (2006) [Pubmed]
  20. Adhesion molecules in hematopoietic cells. Kinashi, T., Springer, T.A. Blood Cells (1994) [Pubmed]
  21. Role of stem cell factor and c-kit signaling in regulation of fetal intestinal epithelial cell adhesion to fibronectin. Shimizu, M., Minakuchi, K., Tsuda, A., Hiroi, T., Tanaka, N., Koga, J., Kiyono, H. Exp. Cell Res. (2001) [Pubmed]
  22. Differential utilization of VLA-4 (alpha 4 beta 1) and -5 (alpha 5 beta 1) integrins during the development of mouse bone marrow-derived mast cells. Fehlner-Gardiner, C.C., Uniyal, S., von Ballestrem, C.G., Chan, B.M. Differentiation (1996) [Pubmed]
  23. Mouse CD24 as a signaling molecule for integrin-mediated cell binding: functional and physical association with src-kinases. Sammar, M., Gulbins, E., Hilbert, K., Lang, F., Altevogt, P. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
  24. Impaired migration of NOD mouse thymocytes: a fibronectin receptor-related defect. Cotta-de-Almeida, V., Villa-Verde, D.M., Lepault, F., Pléau, J.M., Dardenne, M., Savino, W. Eur. J. Immunol. (2004) [Pubmed]
  25. IgE alone stimulates mast cell adhesion to fibronectin via pathways similar to those used by IgE + antigen but distinct from those used by Steel factor. Lam, V., Kalesnikoff, J., Lee, C.W., Hernandez-Hansen, V., Wilson, B.S., Oliver, J.M., Krystal, G. Blood (2003) [Pubmed]
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