The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

ITGA5  -  integrin, alpha 5 (fibronectin receptor,...

Homo sapiens

Synonyms: CD49 antigen-like family member E, CD49e, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ITGA5


High impact information on ITGA5

  • The VLA-5 ligand vascular-cell adhesion molecule-1 mediated preDC2 adhesion/transmigration [5].
  • This paper shows that the common VLA beta-subunit is equivalent to subunits found in structures with known fibronectin and laminin receptor activity, and that VLA-3 and VLA-5 are similar or identical to these previously defined receptors for adhesion molecules [6].
  • Migration requires the engagement of VLA4 in cooperation with VLA5 and both receptors regulate the persistence and directionality of movement [7].
  • Herein, we show that the cytokines interleukin (IL)-3, granulocyte-macrophage CSF (GM-CSF), and KIT ligand (KL) are physiological activators of VLA-4 and VLA-5 expressed by MO7e, TF1, and normal bone marrow CD34+ progenitor cells [8].
  • The VLA-5 fibronectin receptor was mainly expressed on CD4+ CD45R-CDw29+ cells and may in part contribute to the unique function of these cells [9].

Chemical compound and disease context of ITGA5


Biological context of ITGA5


Anatomical context of ITGA5

  • Incubating monocytes with postreperfusion cardiac lymph that contained these FN fragments selectively reduced expression of VLA-5, an effect suppressed by specific immunoadsorption of the fragments [15].
  • In the binding assays, the numbers of FDCs bound to fibronectin- and laminin-coated dishes and LFs of cryostat sections of human tonsils were reduced markedly by pretreatment with monoclonal antibodies against CD29, CD49e, and CD49f [16].
  • Fibronectin promotes proliferation of naive and memory T cells by signaling through both the VLA-4 and VLA-5 integrin molecules [17].
  • These mast cells were found to express VLA (very late Ag)-3 (75.3 +/- 35.6 specific fluorescence intensity) and, to lesser degree, VLA-4 and VLA-5 receptors (8.0 +/- 2.5 and 6.9 +/- 3.2 specific fluorescence intensity, respectively) [18].
  • IL-6 also enhanced the fluorescence intensity of beta 1 integrins, CD49d, CD49e, and CD49f, expressed on NK cells [19].

Associations of ITGA5 with chemical compounds

  • Treating monocytes with purified, 120-kDa cell-binding FN fragments (FN120) likewise decreased VLA-5 expression, and did so by inducing a serine proteinase-dependent proteolysis of this beta(1) integrin [15].
  • Abs to the beta(1) integrins, CD49d and CD49e, as well as to P-selectin and P-selectin glycoprotein ligand 1, inhibited basophil rolling and adhesion [20].
  • Down-modulation of VLA-5 on the apical surface of monocytes by plating the cells onto surfaces precoated with anti-VLA-5 mAb also inhibited binding of beads coated with maltose-binding protein-FimD to monocytes, while precoating of the surfaces with mAb against VLA-6 or CR3 had no effect [2].
  • These results indicate that chemotaxis of PMNs in response to TGF-beta isoforms is mediated by the interaction of the Arg-gly-Asp-ser sequence in the CBD of Fn with an integrin on the PMN cell surface, primarily the VLA-5 integrin [21].
  • The tyrosine phosphorylation of polypeptides of 130 kDa and 77 kDa was triggered in U-937 cells by the interaction of FN with the VLA-5 receptor in a high-affinity conformation [22].

Physical interactions of ITGA5

  • Analysis of binding mechanisms indicated that very late antigen-4 (VLA-4) and VLA-5 were responsible for AML cell binding to fibronectin [23].
  • K562 lacked VLA-4 and a low binding affinity of the VLA-5 on these cells resulted in an absence of binding to the bone-marrow stroma [24].

Regulatory relationships of ITGA5


Other interactions of ITGA5

  • Fibronectin fragments modulate monocyte VLA-5 expression and monocyte migration [15].
  • The majority of leukemic blasts studied expressed CD49d and CD49e as well [30].
  • Immunoelectron microscopy revealed fibers surrounded by cytoplasmic FDC extensions that were CD29-, CD49e-, and CD49f-positive [16].
  • High surface expression of CD49e, CD51, and CD61 along with kit was apparent by 4 weeks of culture, whereas expression of each at day 0 was low to undetectable [31].
  • Using a panel of specific antibodies we showed that freshly harvested human epidermal basal cells express VLA-2 and VLA-3 receptors, a low amount of VLA-5, but fail to express VLA-4 [32].

Analytical, diagnostic and therapeutic context of ITGA5


  1. Adhesion receptors on peripheral blood leukemic B cells. A comparative study on B cell chronic lymphocytic leukemia and related lymphoma/leukemias. Csanaky, G., Matutes, E., Vass, J.A., Morilla, R., Catovsky, D. Leukemia (1997) [Pubmed]
  2. Binding of FimD on Bordetella pertussis to very late antigen-5 on monocytes activates complement receptor type 3 via protein tyrosine kinases. Hazenbos, W.L., van den Berg, B.M., Geuijen, C.W., Mooi, F.R., van Furth, R. J. Immunol. (1995) [Pubmed]
  3. Differential use of very late antigen-4 and -5 integrins by hematopoietic precursors and myeloma cells to adhere to transforming growth factor-beta1-treated bone marrow stroma. Robledo, M.M., Sanz-Rodriguez, F., Hidalgo, A., Teixidó, J. J. Biol. Chem. (1998) [Pubmed]
  4. Adhesion molecules VLA-1 to VLA-6 define discrete stages of peripheral B lymphocyte development and characterize different types of B cell neoplasia. Möller, P., Eichelmann, A., Koretz, K., Mechtersheimer, G. Leukemia (1992) [Pubmed]
  5. Stromal-derived factor-1 in human tumors recruits and alters the function of plasmacytoid precursor dendritic cells. Zou, W., Machelon, V., Coulomb-L'Hermin, A., Borvak, J., Nome, F., Isaeva, T., Wei, S., Krzysiek, R., Durand-Gasselin, I., Gordon, A., Pustilnik, T., Curiel, D.T., Galanaud, P., Capron, F., Emilie, D., Curiel, T.J. Nat. Med. (2001) [Pubmed]
  6. Fibronectin receptor structures in the VLA family of heterodimers. Takada, Y., Huang, C., Hemler, M.E. Nature (1987) [Pubmed]
  7. Cell adhesion and migration are regulated at distinct stages of thymic T cell development: the roles of fibronectin, VLA4, and VLA5. Crisa, L., Cirulli, V., Ellisman, M.H., Ishii, J.K., Elices, M.J., Salomon, D.R. J. Exp. Med. (1996) [Pubmed]
  8. Cytokines increase human hemopoietic cell adhesiveness by activation of very late antigen (VLA)-4 and VLA-5 integrins. Lévesque, J.P., Leavesley, D.I., Niutta, S., Vadas, M., Simmons, P.J. J. Exp. Med. (1995) [Pubmed]
  9. Activation of CD4 cells by fibronectin and anti-CD3 antibody. A synergistic effect mediated by the VLA-5 fibronectin receptor complex. Matsuyama, T., Yamada, A., Kay, J., Yamada, K.M., Akiyama, S.K., Schlossman, S.F., Morimoto, C. J. Exp. Med. (1989) [Pubmed]
  10. Mobilization of myeloma cells involves SDF-1/CXCR4 signaling and downregulation of VLA-4. Gazitt, Y., Akay, C. Stem Cells (2004) [Pubmed]
  11. Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect and induction of selectin-ligand carbohydrates. Koike, T., Kimura, N., Miyazaki, K., Yabuta, T., Kumamoto, K., Takenoshita, S., Chen, J., Kobayashi, M., Hosokawa, M., Taniguchi, A., Kojima, T., Ishida, N., Kawakita, M., Yamamoto, H., Takematsu, H., Suzuki, A., Kozutsumi, Y., Kannagi, R., Kanangi, R. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  12. Assignment of three rat integrin genes to chromosome 19 (ITGB1), chromosome 3 (ITGA4), and chromosome 7 (ITGA5). Szpirer, C., Rivière, M., Szpirer, J., Levan, G., Jaspers, M., Vekemans, S., Cassiman, J.J. Mamm. Genome (1992) [Pubmed]
  13. Sequential regulation of alpha 4 beta 1 and alpha 5 beta 1 integrin avidity by CC chemokines in monocytes: implications for transendothelial chemotaxis. Weber, C., Alon, R., Moser, B., Springer, T.A. J. Cell Biol. (1996) [Pubmed]
  14. Laminin and fibronectin promote the chemotaxis of human malignant plasma cell lines. Shibayama, H., Tagawa, S., Hattori, H., Inoue, R., Katagiri, S., Kitani, T. Blood (1995) [Pubmed]
  15. Fibronectin fragments modulate monocyte VLA-5 expression and monocyte migration. Trial, J., Baughn, R.E., Wygant, J.N., McIntyre, B.W., Birdsall, H.H., Youker, K.A., Evans, A., Entman, M.L., Rossen, R.D. J. Clin. Invest. (1999) [Pubmed]
  16. Follicular dendritic cells adhere to fibronectin and laminin fibers via their respective receptors. Ogata, T., Yamakawa, M., Imai, Y., Takahashi, T. Blood (1996) [Pubmed]
  17. Fibronectin promotes proliferation of naive and memory T cells by signaling through both the VLA-4 and VLA-5 integrin molecules. Davis, L.S., Oppenheimer-Marks, N., Bednarczyk, J.L., McIntyre, B.W., Lipsky, P.E. J. Immunol. (1990) [Pubmed]
  18. Human skin mast cells express functional beta 1 integrins that mediate adhesion to extracellular matrix proteins. Columbo, M., Bochner, B.S., Marone, G. J. Immunol. (1995) [Pubmed]
  19. Response of human NK cells to IL-6 alterations of the cell surface phenotype, adhesion to fibronectin and laminin, and tumor necrosis factor-alpha/beta secretion. Rabinowich, H., Sedlmayr, P., Herberman, R.B., Whiteside, T.L. J. Immunol. (1993) [Pubmed]
  20. Stimulation of Human Endothelium with IL-3 Induces Selective Basophil Accumulation In Vitro. Lim, L.H., Burdick, M.M., Hudson, S.A., Mustafa, F.B., Konstantopoulos, K., Bochner, B.S. J. Immunol. (2006) [Pubmed]
  21. Neutrophil chemotaxis in response to TGF-beta isoforms (TGF-beta 1, TGF-beta 2, TGF-beta 3) is mediated by fibronectin. Parekh, T., Saxena, B., Reibman, J., Cronstein, B.N., Gold, L.I. J. Immunol. (1994) [Pubmed]
  22. Post-receptor occupancy events in leukocytes during beta 1 integrin-ligand interactions. Sánchez-Mateos, P., Arroyo, A.G., Balboa, M.A., Sánchez-Madrid, F. Eur. J. Immunol. (1993) [Pubmed]
  23. Human acute myeloid leukemia cells bind to bone marrow stroma via a combination of beta-1 and beta-2 integrin mechanisms. Bendall, L.J., Kortlepel, K., Gottlieb, D.J. Blood (1993) [Pubmed]
  24. beta1-Integrins dominate cell traffic of leukemic cells in human bone-marrow stroma. Van der Velde-Zimmerman, D., Smits, A.J., Verdaasdonk, M.A., Rademakers, L.H., Werner, N., Spierings, D.C., De Weger, R.A., Van den Tweel, J.G., Joling, P. Int. J. Cancer (1996) [Pubmed]
  25. Interactions of dendritic cells with fibronectin and endothelial cells. Jancic, C., Chuluyan, H.E., Morelli, A., Larregina, A., Kolkowski, E., Saracco, M., Barboza, M., Leiva, W.S., Fainboim, L. Immunology (1998) [Pubmed]
  26. Signal transduction through the beta1 integrin family surface adhesion molecules VLA-4 and VLA-5 of human B-cell precursors activates CD19 receptor-associated protein-tyrosine kinases. Xiao, J., Messinger, Y., Jin, J., Myers, D.E., Bolen, J.B., Uckun, F.M. J. Biol. Chem. (1996) [Pubmed]
  27. Cell cycle activation of hematopoietic progenitor cells increases very late antigen-5-mediated adhesion to fibronectin. Giet, O., Huygen, S., Beguin, Y., Gothot, A. Exp. Hematol. (2001) [Pubmed]
  28. Bordetella pertussis infection of human respiratory epithelial cells up-regulates intercellular adhesion molecule-1 expression: role of filamentous hemagglutinin and pertussis toxin. Ishibashi, Y., Nishikawa, A. Microb. Pathog. (2002) [Pubmed]
  29. Flt3-ligand induces adhesion of haematopoietic progenitor cells via a very late antigen (VLA)-4- and VLA-5-dependent mechanism. Solanilla, A., Grosset, C., Duchez, P., Legembre, P., Pitard, V., Dupouy, M., Belloc, F., Viallard, J.F., Reiffers, J., Boiron, J.M., Coulombel, L., Ripoche, J. Br. J. Haematol. (2003) [Pubmed]
  30. Expression of integrins and examination of their adhesive function in normal and leukemic hematopoietic cells. Liesveld, J.L., Winslow, J.M., Frediani, K.E., Ryan, D.H., Abboud, C.N. Blood (1993) [Pubmed]
  31. Human mast cells derived from fetal liver cells cultured with stem cell factor express a functional CD51/CD61 (alpha v beta 3) integrin. Shimizu, Y., Irani, A.M., Brown, E.J., Ashman, L.K., Schwartz, L.B. Blood (1995) [Pubmed]
  32. Identification of specific human epithelial cell integrin receptors as VLA proteins. Staquet, M.J., Levarlet, B., Dezutter-Dambuyant, C., Schmitt, D., Thivolet, J. Exp. Cell Res. (1990) [Pubmed]
  33. Adhesion of precursor-B acute lymphoblastic leukaemia cells to bone marrow stromal proteins. Makrynikola, V., Bradstock, K.F. Leukemia (1993) [Pubmed]
  34. Cell-adhesion molecules expressed by activated eosinophils in Onchocerca volvulus infection. Brattig, N.W., Abakar, A.Z., Geisinger, F., Kruppa, T.F. Parasitol. Res. (1995) [Pubmed]
  35. Immunostaining of human brain capillaries by antibodies to very late antigens. McGeer, P.L., Zhu, S.G., Dedhar, S. J. Neuroimmunol. (1990) [Pubmed]
  36. Correlation of very late activation integrin and CD44 expression with extrarenal invasion and metastasis of renal cell carcinomas. Gilcrease, M.Z., Truong, L., Brown, R.W. Hum. Pathol. (1996) [Pubmed]
  37. Attachment of intrinsically and extrinsically aged fibroblasts on collagen and fibronectin. Püschel, H.U., Chang, J., Müller, P.K., Brinckmann, J. J. Photochem. Photobiol. B, Biol. (1995) [Pubmed]
WikiGenes - Universities