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GOLGA4  -  golgin A4

Homo sapiens

Synonyms: 256 kDa golgin, CRPF46, GCP2, GOLG, Golgin subfamily A member 4, ...
 
 
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Disease relevance of GOLGA4

 

High impact information on GOLGA4

  • The structure is consistent with golgin-245 forming parallel coiled-coils and suggests how Arl1-GTP/GRIP complexes interact with Golgi membranes via the N termini of Arl1-GTP and the C-terminal tails of the GRIP domains [6].
  • Only the acrosomal cap and the principal piece of the tail were decorated with rabbit and hydridoma antibodies against an immunoanalogue of erythrocyte alpha-spectrin (p230). p230 appeared to be the major calmodulin-binding polypeptide in spermatozoa, as shown by a direct overlay assay of electrophoretic blots of spermatozoa with 125I-calmodulin [7].
  • The p36 substrate of tyrosine-specific protein kinases co-localizes with non-erythrocyte alpha-spectrin antigen, p230, in surface lamina of cultured fibroblasts [8].
  • Upon demecolcine-induced reorganization of intermediate filaments, however, the localization of p230 was rapidly altered to a dense plaque underneath the perinuclear aggregate of intermediate filaments [1].
  • We have used here polyclonal antisera and monoclonal antibodies in indirect immunofluorescence microscopy to study the subcellular location of p36 and the p230, which is a subplasmalemmal polypeptide showing immunologic cross-reactivity with erythrocyte alpha-spectrin [8].
 

Biological context of GOLGA4

  • Collectively these results show that GCC88, GCC185 and p230/golgin245 are recruited to functionally distinct domains of the TGN and are likely to be important for the maintenance of TGN subdomain structure, a critical feature for mediating protein sorting and membrane transport [9].
  • To identify molecules that interact with the flexible amino-terminal end of p230, we used this domain as bait to screen a human brain cDNA library in a yeast two-hybrid assay [10].
  • Molecular characterization of trans-Golgi p230. A human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil alpha-helical domains and a granin motif [11].
  • The role of G protein activators on the binding of p230 to Golgi membranes and in vesicle biogenesis has been investigated [12].
  • By analyzing more than 30 mutants of golgin-97 and golgin-245 GRIP domains for their properties of dimerization, interaction with ARF like protein 1 (Arl1)-GTP and Golgi targeting, we found hierarchically organized three-tier interactions governing the Golgi targeting of GRIP domain golgins [13].
 

Anatomical context of GOLGA4

  • Previously, using human autoimmune sera, we identified and characterized a TGN protein, p230/Golgin-245, an extensively coiled-coil protein with flexible amino- and carboxyl-terminal ends, that is anchored to TGN membranes and TGN-derived vesicles by its carboxyl-terminal GRIP domain [10].
  • Previously, we have identified a carboxy-terminal domain of the trans-Golgi-network (TGN) protein p230 that is responsible for Golgi localisation [1] [14].
  • Molecular characterization of Golgin-245, a novel Golgi complex protein containing a granin signature [15].
  • We have previously described a brefeldin-A sensitive, hydrophilic protein (p230), containing a very high frequency of heptad repeats, found in the cytosol and associated with Golgi membranes [12].
  • Treatment of streptolysin-O permeabilised HeLa cells with either GTP gamma S or AlF4- resulted in accumulation of p230 on Golgi membranes [12].
 

Associations of GOLGA4 with chemical compounds

  • Interaction between p230 and MACF1 is associated with transport of a glycosyl phosphatidyl inositol-anchored protein from the Golgi to the cell periphery [10].
  • Giant vacuole formation, of confirmed trans-Golgi origin (labeled with C5-ceramide, p230, golgin-97), is a cellular response to all tested amines in the series (> or = 2.5 mM), including triethylamine [16].
  • Both p36 and p230 showed a diffuse distribution in fixed and permeabilized cells and were localized in a surface lamina-like network in Triton-extracted cells [8].
  • The alternative splicing occurs within the first proline-rich domain of p230 [5].
 

Regulatory relationships of GOLGA4

  • Endogenous p230 was displaced from the Golgi membranes in transfected cells expressing high levels of GFP fused to the GLD of either p230 or golgin-97, indicating that different GLDs interact with similar membrane determinants [14].
 

Other interactions of GOLGA4

 

Analytical, diagnostic and therapeutic context of GOLGA4

  • Here we report the molecular cloning and sequence analysis of human p230 and the localization of its gene to chromosome 6p12 22 [11].
  • The e1a2, b2a2 or b3a2 and c3a2 fusion mRNAs encode distinct fusion proteins (p190, p210 and p230, respectively), which are associated with different forms of leukemogenesis in humans and animal models [19].
  • RT-PCR analysis indicated that the splicing occurs independently of previously reported carboxyl-terminal splicing, and that this novel splice variant is more frequent than the previously reported p230 [5].
  • GFP fusion proteins containing either the T. brucei GRIP domain or the human p230 GRIP (p230GRIP) domain were also expressed in the trypanosomatid parasite, Leishmania mexicana, and localized by fluorescence and immuno-electron microscopy to the trans face of the single Golgi apparatus and a short tubule that extended from the Golgi apparatus [20].

References

  1. Immunolocalization of a novel, cytoskeleton-associated polypeptide of Mr 230,000 daltons (p230). Lehto, V.P., Virtanen, I. J. Cell Biol. (1983) [Pubmed]
  2. Establishment and molecular characterization of a novel leukemic cell line with Philadelphia chromosome expressing p230 BCR/ABL fusion protein. Wada, H., Mizutani, S., Nishimura, J., Usuki, Y., Kohsaki, M., Komai, M., Kaneko, H., Sakamoto, S., Delia, D., Kanamaru, A. Cancer Res. (1995) [Pubmed]
  3. Bcr-abl-positive cells secrete angiogenic factors including matrix metalloproteinases and stimulate angiogenesis in vivo in Matrigel implants. Janowska-Wieczorek, A., Majka, M., Marquez-Curtis, L., Wertheim, J.A., Turner, A.R., Ratajczak, M.Z. Leukemia (2002) [Pubmed]
  4. p230 does not always predict a mild clinical course in myeloid malignancies: e19a2 bcr/abl fusion transcript with additional chromosome abnormalities in a patient with acute monoblastic leukemia (M5a). Bernasconi, P., Calatroni, S., Boni, M., Cavigliano, P.M., Pagnucco, G., Bernasconi, C. Haematologica (2001) [Pubmed]
  5. Novel variant of p230 trans-Golgi network protein identified by serum from Sjögren's syndrome patient. Tsukada, Y., Ichikawa, H., Chai, Z., Lai, F.P., Dunster, K., Sentry, J.W., Toh, B.H. Eur. J. Cell Biol. (2000) [Pubmed]
  6. Structural basis for Arl1-dependent targeting of homodimeric GRIP domains to the Golgi apparatus. Panic, B., Perisic, O., Veprintsev, D.B., Williams, R.L., Munro, S. Mol. Cell (2003) [Pubmed]
  7. Distinct cytoskeletal domains revealed in sperm cells. Virtanen, I., Badley, R.A., Paasivuo, R., Lehto, V.P. J. Cell Biol. (1984) [Pubmed]
  8. The p36 substrate of tyrosine-specific protein kinases co-localizes with non-erythrocyte alpha-spectrin antigen, p230, in surface lamina of cultured fibroblasts. Lehto, V.P., Virtanen, I., Paasivuo, R., Ralston, R., Alitalo, K. EMBO J. (1983) [Pubmed]
  9. Mammalian GRIP domain proteins differ in their membrane binding properties and are recruited to distinct domains of the TGN. Derby, M.C., van Vliet, C., Brown, D., Luke, M.R., Lu, L., Hong, W., Stow, J.L., Gleeson, P.A. J. Cell. Sci. (2004) [Pubmed]
  10. Interaction between p230 and MACF1 is associated with transport of a glycosyl phosphatidyl inositol-anchored protein from the Golgi to the cell periphery. Kakinuma, T., Ichikawa, H., Tsukada, Y., Nakamura, T., Toh, B.H. Exp. Cell Res. (2004) [Pubmed]
  11. Molecular characterization of trans-Golgi p230. A human peripheral membrane protein encoded by a gene on chromosome 6p12-22 contains extensive coiled-coil alpha-helical domains and a granin motif. Erlich, R., Gleeson, P.A., Campbell, P., Dietzsch, E., Toh, B.H. J. Biol. Chem. (1996) [Pubmed]
  12. p230 is associated with vesicles budding from the trans-Golgi network. Gleeson, P.A., Anderson, T.J., Stow, J.L., Griffiths, G., Toh, B.H., Matheson, F. J. Cell. Sci. (1996) [Pubmed]
  13. Multilayer Interactions Determine the Golgi Localization of GRIP Golgins. Lu, L., Tai, G., Wu, M., Song, H., Hong, W. Traffic (2006) [Pubmed]
  14. A novel Golgi-localisation domain shared by a class of coiled-coil peripheral membrane proteins. Kjer-Nielsen, L., Teasdale, R.D., van Vliet, C., Gleeson, P.A. Curr. Biol. (1999) [Pubmed]
  15. Molecular characterization of Golgin-245, a novel Golgi complex protein containing a granin signature. Fritzler, M.J., Lung, C.C., Hamel, J.C., Griffith, K.J., Chan, E.K. J. Biol. Chem. (1995) [Pubmed]
  16. N-substituted 4-aminobenzamides (procainamide analogs): an assessment of multiple cellular effects concerning ion trapping. Morissette, G., Moreau, E., C-Gaudreault, R., Marceau, F. Mol. Pharmacol. (2005) [Pubmed]
  17. Interaction of Arl1-GTP with GRIP domains recruits autoantigens Golgin-97 and Golgin-245/p230 onto the Golgi. Lu, L., Hong, W. Mol. Biol. Cell (2003) [Pubmed]
  18. Sequence analysis of a 685-kb genomic region on chromosome 3p22-p21.3 that is homozygously deleted in a lung carcinoma cell line. Ishikawa, S., Kai, M., Tamari, M., Takei, Y., Takeuchi, K., Bandou, H., Yamane, Y., Ogawa, M., Nakamura, Y. DNA Res. (1997) [Pubmed]
  19. Pre-B acute lymphoblastic leukemia with b3a2 (p210) and e1a2 (p190) BCR-ABL fusion transcripts relapsing as chronic myelogenous leukemia with a less differentiated b3a2 (p210) clone. Winter, S.S., Greene, J.M., McConnell, T.S., Willman, C.L. Leukemia (1999) [Pubmed]
  20. Targeting of the GRIP domain to the trans-Golgi network is conserved from protists to animals. McConville, M.J., Ilgoutz, S.C., Teasdale, R.D., Foth, B.J., Matthews, A., Mullin, K.A., Gleeson, P.A. Eur. J. Cell Biol. (2002) [Pubmed]
 
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