Disease relevance of Pebp1

• Both treatments, on chronic administration, increased mRNA levels of the transcription factor polyomavirus enhancer-binding protein (PEBP) 2beta in frontal cortex (FCx) [1].

Psychiatry related information on Pebp1

• Furthermore, several lines of evidence obtained from studies of humans and animal models suggest that certain types of memory and learning disorders are associated with abnormal accumulation and expression of HCNP analogue peptide and/or its precursor protein mRNA in the hippocampus [2].

High impact information on Pebp1

• One of these components, originally purified from young rat hippocampus, is a novel undecapeptide (hippocampal cholinergic neurostimulating peptide; HCNP); this enhances the production of ChAT, but not of AchE [2].
• The amino acid sequence deduced from base sequence analysis of cloned HCNP-precursor protein cDNA shows that HCNP is located at the N-terminal domain of its precursor protein [2].
• The 21 kDa HCNP precursor protein shows homology with other proteins, and it functions not only as an HCNP precursor, but also as a binding protein for ATP, opioids and phosphatidylethanolamine [2].
• In keeping with its multiple functions, the multiple enhancers and promoters found in the genomic DNA for HCNP precursor protein may be involved in the regulation of its gene in a variety of cells and at different stages of development [2].
• These findings suggest that the intracellular phosphatidylethanolamine-binding protein could be one of the factors responsible for the resistance of cKDH-8/11 cells to TNF-alpha-induced cell death [3].

Biological context of Pebp1

• These data suggest that PBP may be involved in the organisation of sperm membranes during spermiogenesis [4].
• Phosphatidylethanolamine-binding protein (PBP) has been described previously in the male reproductive tract, where it has been implicated in the biogenesis and maintenance of antigen segregation of membranes [4].
• Sequence homology of rat and human HCNP precursor proteins, bovine phosphatidylethanolamine-binding protein and rat 23-kDa protein associated with the opioid-binding protein [5].
• In prepubertal animals, PBP was expressed in both testes and epididymides from day 1 and day 3 postpartum, respectively (day 3 being the earliest epididymal tissue taken) [4].
• Phosphatidylethanolamine binding protein is an abundant secretory product of haploid testicular germ cells in the rat [6].

Anatomical context of Pebp1

• Hippocampal cholinergic neurostimulating peptide (HCNP), which derives from phosphatidylethanolamine-binding protein (also named Raf kinase inhibitor protein), enhances acetylcholine synthesis in the hippocampal medial septal nuclei [7].
• In the adult rat testis, PBP was localised to step 17-19 elongating spermatids, residual bodies, and interstitial Leydig cells [4].
• In the adult epididymis, PBP was localised to epithelial cells of the caput, corpus, and cauda regions and to the cytoplasmic droplets of spermatozoa in the lumen of the initial segment, caput, and corpus epididymidis [4].
• In prepubertal testes, PBP was localised to Leydig cells from day 1 postpartum and was not detected in any other cell type until the differentiation of elongate spermatids, when it was detected in step 17-19 elongating spermatids [4].
• The presence of PBP in Leydig cells, however, suggests diverse roles for this protein as a lipid carrier or binding protein [4].

Associations of Pebp1 with chemical compounds

• A 40 amino acid sequence of a cyanogen bromide cleaved internal fragment of RSP-24.5 showed 80.5% homology to a phosphatidylethanolamine binding protein [8].

Other interactions of Pebp1

• Expression of phosphatidylethanolamine-binding protein in the male reproductive tract: immunolocalisation and expression in prepubertal and adult rat testes and epididymides [4].
• The presence of PEBP and FKBP12 in conditioned medium was confirmed immunologically [9].
• These results suggest that phosphatidylethanolamine binding protein participates in the negative regulation of Sertoli cell secretory function during spermatogenesis [8].
• Among proteins in the corpus striatum, frontal lobe, hippocampus and thalamus/hypothalamus, ubiquitin C-terminal hydrolase L1 and peroxiredoxin 2 decreased in the hippocampus and phosphatidylethanolamine-binding protein increased in the thalamus/hypothalamus of rats with the enhanced rebound hyperphargia induced by space-restriction [10].

Analytical, diagnostic and therapeutic context of Pebp1

• In the present study we have used a specific antiserum to PBP to determine its expression and localisation in the adult and prepubertal rat testis and epididymis by Western blotting and immunohistochemistry [4].
• The development of specific immunoassays for germ cell-secreted proteins (e.g., PEBP and ARP-2) should enable more definitive assessment of whether proteins secreted by the seminiferous epithellum can be measured in blood and thus provide a potential means of monitoring spermatogenesis [11].
• On Northern blots, PEBP mRNA was abundant in adult rat testis and epididymis and fractions enriched in germ cells but was very low/absent from fetal or immature rat testis or adult rat Sertoli cells [6].

References