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Gene Review

TUBA1A  -  tubulin, alpha 1a

Bos taurus

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High impact information on LOC404053


Biological context of LOC404053

  • Although recent studies have implicated beta-tubulin as the site of PTX binding, no information is available that relates alpha-tubulin to the binding site [6].
  • Differential assembly kinetics of alpha-tubulin isoforms in the presence of paclitaxel [6].
  • The peptides were modeled after the known, or putative, tyrosine sulfation sites of the cholecystokinin precursor, chromogranin B (secretogranin I) and vitronectin, as well as the tyrosine phosphorylation sites of alpha-tubulin and pp60src [7].
  • Separate alignments of 75 alpha-tubulin, 106 beta-tubulin, and 14 gamma-tubulin sequences and 12 sequences of the bacterial cell division protein FtsZ have been employed to predict their secondary structures with the multiple-sequence method PHD [Rost, B., & Sander, C. (1993a) J. Mol. Biol. 232, 584-599] [8].
  • Microtubule configurations and post-translational alpha-tubulin modifications during mammalian spermatogenesis [9].

Anatomical context of LOC404053


Associations of LOC404053 with chemical compounds

  • Tyrosination-detyrosination is one of the major posttranslational modifications in which the C-terminal tyrosine residue in alpha-tubulin is added or removed reversibly [5].
  • Direct photoaffinity labeling of cysteine-295 of alpha-tubulin by guanosine 5'-triphosphate bound in the nonexchangeable site [12].
  • A large cyanogen bromide peptide was recovered, and its further degradation with endoproteinase Glu-C established that cysteine-295 of alpha-tubulin was the major reactive amino acid cross-linked to guanosine by ultraviolet irradiation [12].
  • This domain includes the cysteine residues at positions 295, 305, 315 and 316 on alpha-tubulin; these residues are located well away from the alpha/beta interface where colchicine appears to bind [13].
  • Two photoaffinity analogues of the tripeptide, hemiasterlin, exclusively label alpha-tubulin [14].

Physical interactions of LOC404053

  • Polymers composed of normal alpha-tubulin and cleaved beta-tubulin or of cleaved alpha- and beta-tubulins were stabilized in the presence of added MAP2, myelin basic protein and histone H1 [15].
  • In this study we have used limited proteolysis and cross-linking analysis to localize further the stathmin-binding site on alpha-tubulin [16].

Other interactions of LOC404053


Analytical, diagnostic and therapeutic context of LOC404053


  1. Microtubules containing detyrosinated tubulin are less dynamic. Kreis, T.E. EMBO J. (1987) [Pubmed]
  2. Alpha-tubulin acetylase activity in isolated Chlamydomonas flagella. Greer, K., Maruta, H., L'Hernault, S.W., Rosenbaum, J.L. J. Cell Biol. (1985) [Pubmed]
  3. Different early-signaling pathways coupled to transcriptional and posttranscriptional regulation of gene expression during mitogenic activation of T lymphocytes. White, M.W., Oberhauser, A.K., Kuepfer, C.A., Morris, D.R. Mol. Cell. Biol. (1987) [Pubmed]
  4. Dinitroanilines bind alpha-tubulin to disrupt microtubules. Morrissette, N.S., Mitra, A., Sept, D., Sibley, L.D. Mol. Biol. Cell (2004) [Pubmed]
  5. Coordination of posttranslational modifications of bovine brain alpha-tubulin. Polyglycylation of delta2 tubulin. Banerjee, A. J. Biol. Chem. (2002) [Pubmed]
  6. Differential assembly kinetics of alpha-tubulin isoforms in the presence of paclitaxel. Banerjee, A., Kasmala, L.T. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  7. Analysis of the substrate specificity of tyrosylprotein sulfotransferase using synthetic peptides. Niehrs, C., Kraft, M., Lee, R.W., Huttner, W.B. J. Biol. Chem. (1990) [Pubmed]
  8. Tubulin secondary structure analysis, limited proteolysis sites, and homology to FtsZ. de Pereda, J.M., Leynadier, D., Evangelio, J.A., Chacón, P., Andreu, J.M. Biochemistry (1996) [Pubmed]
  9. Microtubule configurations and post-translational alpha-tubulin modifications during mammalian spermatogenesis. Moreno, R.D., Schatten, G. Cell Motil. Cytoskeleton (2000) [Pubmed]
  10. Asymmetric distribution of functional sodium-calcium exchanger in primary osteoblasts. Stains, J.P., Gay, C.V. J. Bone Miner. Res. (1998) [Pubmed]
  11. Tubulin: an integral protein of mammalian synaptic vesicle membranes. Zisapel, N., Levi, M., Gozes, I. J. Neurochem. (1980) [Pubmed]
  12. Direct photoaffinity labeling of cysteine-295 of alpha-tubulin by guanosine 5'-triphosphate bound in the nonexchangeable site. Bai, R., Choe, K., Ewell, J.B., Nguyen, N.Y., Hamel, E. J. Biol. Chem. (1998) [Pubmed]
  13. The interaction of the B-ring of colchicine with alpha-tubulin: a novel footprinting approach. Chaudhuri, A.R., Seetharamalu, P., Schwarz, P.M., Hausheer, F.H., Ludueña, R.F. J. Mol. Biol. (2000) [Pubmed]
  14. Two photoaffinity analogues of the tripeptide, hemiasterlin, exclusively label alpha-tubulin. Nunes, M., Kaplan, J., Wooters, J., Hari, M., Minnick, A.A., May, M.K., Shi, C., Musto, S., Beyer, C., Krishnamurthy, G., Qiu, Y., Loganzo, F., Ayral-Kaloustian, S., Zask, A., Greenberger, L.M. Biochemistry (2005) [Pubmed]
  15. Stabilization and bundling of subtilisin-treated microtubules induced by microtubule associated proteins. Saoudi, Y., Paintrand, I., Multigner, L., Job, D. J. Cell. Sci. (1995) [Pubmed]
  16. OP18/stathmin binds near the C-terminus of tubulin and facilitates GTP binding. Moreno, F.J., Bagnat, M., Lim, F., Avila, J. Eur. J. Biochem. (1999) [Pubmed]
  17. Effects of mechanical stress on the mRNA expression of S100A4 and cytoskeletal components by periodontal ligament cells. Duarte, W.R., Mikuni-Takagaki, Y., Kawase, T., Limura, T., Oida, S., Ohya, K., Takenaga, K., Ishikawa, L., Kasugai, S. J. Med. Dent. Sci. (1999) [Pubmed]
  18. Ultrasound inhibits the adhesion and migration of smooth muscle cells in vitro. Alter, A., Rozenszajn, L.A., Miller, H.I., Rosenschein, U. Ultrasound in medicine & biology. (1998) [Pubmed]
  19. A tyrosine-phosphorylated 55-kilodalton motility-associated bovine sperm protein is regulated by cyclic adenosine 3',5'-monophosphates and calcium. Vijayaraghavan, S., Trautman, K.D., Goueli, S.A., Carr, D.W. Biol. Reprod. (1997) [Pubmed]
  20. A pH- and temperature-dependent cycling method that doubles the yield of microtubule protein. Tiwari, S.C., Suprenant, K.A. Anal. Biochem. (1993) [Pubmed]
  21. Identification and characterization of axopodial tubulins from Echinosphaerium nucleofilum. Little, M., Quinlan, R.A., Hoffman, E.J., Ludueña, R.F. Eur. J. Cell Biol. (1983) [Pubmed]
  22. Contribution of spermatozoal centrosomes to the microtubule-organizing centre in Antarctic minke whale ( Balaenoptera bonaerensis ). Kobayashi, T., Amemiya, K., Takeuchi, K., Tsujioka, T., Tominaga, K., Hirabayashi, M., Ishikawa, H., Fukui, Y., Hochi, S. Zygote (2006) [Pubmed]
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