High impact information on LOC404053

• Peptide antibodies specific for tyrosinated (tyr-tubulin) or detyrosinated alpha-tubulin (glu-tubulin) have been generated for studying the relative stability of microtubules enriched in either form of alpha-tubulin [1].
• We have previously shown that the alpha-tubulin of Chlamydomonas flagella is synthesized as a precursor which is modified by acetylation in the flagellum during flagellar assembly [2].
• The type 2 messages (ornithine decarboxylase, actin, and alpha-tubulin) were regulated posttranscriptionally through activation of protein kinase C [3].
• All 23 of the lines that we examined harbored single point mutations in alpha-tubulin [4].
• Coordination of posttranslational modifications of bovine brain alpha-tubulin. Polyglycylation of delta2 tubulin [5].

Biological context of LOC404053

• Although recent studies have implicated beta-tubulin as the site of PTX binding, no information is available that relates alpha-tubulin to the binding site [6].
• Differential assembly kinetics of alpha-tubulin isoforms in the presence of paclitaxel [6].
• The peptides were modeled after the known, or putative, tyrosine sulfation sites of the cholecystokinin precursor, chromogranin B (secretogranin I) and vitronectin, as well as the tyrosine phosphorylation sites of alpha-tubulin and pp60src [7].
• Separate alignments of 75 alpha-tubulin, 106 beta-tubulin, and 14 gamma-tubulin sequences and 12 sequences of the bacterial cell division protein FtsZ have been employed to predict their secondary structures with the multiple-sequence method PHD [Rost, B., & Sander, C. (1993a) J. Mol. Biol. 232, 584-599] [8].
• Microtubule configurations and post-translational alpha-tubulin modifications during mammalian spermatogenesis [9].

Anatomical context of LOC404053

• Such a strikingly different assembly behavior of the alpha-tubulin isoforms indicates that alpha-tubulin may be involved in the interaction of PTX with microtubules [6].
• Alpha-tubulin acetylase activity in isolated Chlamydomonas flagella [2].
• To understand calcium translocation in osteoblasts, we have determined the location of sodium-calcium (Na-Ca) exchanger (NCX) in relation to actin and alpha-tubulin in primary cultures of avian osteoblasts [10].
• In the presence of ethyleneglycolbis)aminoethyl ether)-N,N'-tetraacetic acid (EGTA) or magnesium in the isolation buffers, the synaptic vesicles contained mainly the alpha-tubulin whereas the beta subunit was less abundant [11].
• Similarly, synaptosomal plasma membranes that were prepared in the presence of EGTA also contained more of alpha-tubulin than of the beta subunit [11].

Associations of LOC404053 with chemical compounds

• Tyrosination-detyrosination is one of the major posttranslational modifications in which the C-terminal tyrosine residue in alpha-tubulin is added or removed reversibly [5].
• Direct photoaffinity labeling of cysteine-295 of alpha-tubulin by guanosine 5'-triphosphate bound in the nonexchangeable site [12].
• A large cyanogen bromide peptide was recovered, and its further degradation with endoproteinase Glu-C established that cysteine-295 of alpha-tubulin was the major reactive amino acid cross-linked to guanosine by ultraviolet irradiation [12].
• This domain includes the cysteine residues at positions 295, 305, 315 and 316 on alpha-tubulin; these residues are located well away from the alpha/beta interface where colchicine appears to bind [13].
• Two photoaffinity analogues of the tripeptide, hemiasterlin, exclusively label alpha-tubulin [14].

Physical interactions of LOC404053

• Polymers composed of normal alpha-tubulin and cleaved beta-tubulin or of cleaved alpha- and beta-tubulins were stabilized in the presence of added MAP2, myelin basic protein and histone H1 [15].
• In this study we have used limited proteolysis and cross-linking analysis to localize further the stathmin-binding site on alpha-tubulin [16].

Other interactions of LOC404053

• In Northern blotting analysis, the expression of S100A4, beta-actin, and alpha-tubulin mRNAs was higher in the PDL from fully erupted and functional bovine teeth than in partially erupted ones [17].
• Sonication was accompanied by the disorganization of alpha-SM actin fibers and diminished distribution of vinculin; tyrosinated alpha tubulin and vimentin appeared unaffected [18].
• In the present study, we investigated the direct effects of cyclical stretching on the mRNA expression of S100A4 and two cytoskeletal components (beta-actin and alpha-tubulin) by PDL cells [17].

Analytical, diagnostic and therapeutic context of LOC404053

• Chromatography through diethylaminoethyl-cellulose and Western blot analysis showed that this 55-kDa protein is not a regulatory subunit of PKA or alpha-tubulin [19].
• 0. Quantitative immunoblotting revealed that there was an almost equal partitioning of tyrosinated and acetylated alpha-tubulin isotypes at the higher pH [20].
• In contrast to Physarum and metazoan tubulins but similar to Tetrahymena tubulin, the axopodial alpha-tubulin had a more basic isoelectric point than the beta-subunit as shown by two dimensional gel electrophoresis [21].
• Vitrified-warmed bovine mature oocytes were subjected to immunostaining against alpha-tubulin 4-6 h after intracytoplasmic injection (ICSI) of 5 mM dithiothreitol-treated spermatozoa [22].

References