Disease relevance of PCQAP

• Tazarotene-induced gene 1 (TIG1) expression in prostate carcinomas and its relationship to tumorigenicity [1].
• The tazarotene-induced gene 1 (TIG1), also known as retinoid acid (RA) receptor-responsive 1 gene was first identified as an RA-responsive gene and was shown to be downregulated in prostate cancer [2].
• In conclusion, following the favourable results obtained in animal models and notwithstanding the pharmacological effects typical of an NSAID, MED15 may rationally be proposed for the treatment of various human colitis conditions and Crohn's disease [3].
• In in vivo indomethacin-induced rat ileitis, MED15 heals better than 5-aminosalicylic acid and sulfasalazine, as well as down-regulating intestinal wall myeloperoxidase content [3].

High impact information on PCQAP

• Colony formation after transfection with the cyclin D1 expression vector was repressed in NIH-3T3, TIG-1, CHO-K1, and HeLa cells, compared with those with mock and cyclin E expression vectors [4].
• Normal human diploid cells, TIG-1, ceased to proliferate at about the 62 population doubling level (PDL) [5].
• Tazarotene-induced gene 1 (TIG1), a novel retinoic acid receptor-responsive gene in skin [6].
• In addition, TRIM11 suppressed ARC105-mediated transcriptional activation induced with TGFbeta in a reporter assay [7].
• The deduced open reading frame of the TIG-1 cDNA encodes for a glutamine repeat-rich protein with a predicted molecular weight of 63kDa [8].

Chemical compound and disease context of PCQAP

• In acetic acid-induced colitis in the rat, levels of malondialdehyde were found to be more markedly reduced with MED15 than with 5-aminosalicylic acid [3].

Biological context of PCQAP

• Recently, DeLuca and colleagues reported that polymorphisms in a trinucleotide repeat in exon 7 of PCQAP were associated with schizophrenia in a case-control study of Italian schizophrenics [9].
• The predicted open reading frame also contains a consensus bipartite nuclear localization signal, though no specific DNA-binding domain is found.The corresponding TIG-1 mRNA is ubiquitously expressed [8].
• Cotransfection assays of K562 cells, using a GAL4-TIG-1 fusion gene and GAL4 operator-CAT, indicate that the TIG-1 protein may have transcriptional regulatory activity when tethered to DNA [8].
• Tazarotene-induced gene-1 (Tig1) and CCAAT/enhancer binding protein-alpha (C/ebpalpha) were chosen for further study [10].
• A transient transfection assay demonstrated that the overexpression of cyclin D1 inhibited DNA synthesis of TIG-1 cells [4].

Anatomical context of PCQAP

• Using polyclonal antibodies obtained from a rabbit immunized with the carboxy-terminal portion of bacterially expressed TIG-1 protein, a polypeptide with molecular weight of 97kDa was identified by Western blot analyses of protein lysates obtained from K562 cells [8].
• A novel glutamine-rich putative transcriptional adaptor protein (TIG-1), preferentially expressed in placental and bone-marrow tissues [8].
• Senescent human diploid fibroblasts, TIG-1, had labelling indices of about 0.5-3% when labelled with [3H]thymidine for 3 days in fresh medium containing 10% fetal bovine serum [11].
• It should be noted that wogonin did not show the inhibitory effect on human fetal lung normal diploid cell line TIG-1, as compared to the inhibition observed in cancer cells [12].
• The cytotoxicity of flavonoids, including apigenin, eriodictyol, 3-hydroxyflavone, kaempherol, luteolin, naringenin, quercetin, rutin, and taxifolin, toward cultured human normal cells, i.e., human lung embryonic fibroblasts (TIG-1) and human umbilical vein endothelial (HUVE) cells, was examined [13].

Associations of PCQAP with chemical compounds

• Amtolmetin guacyl (MED15) is a new non-steroidal anti-inflammatory drug (NSAID) which shares anti-inflammatory, analgesic and antipyretic activity with the other drugs of the NSAID family but which shows, unexpectedly, strong gastroprotective activity similar to misoprostol [3].
• The MED15 enteroprotective effect is related to stimulation of intestinal capsaicin receptors as demonstrated by the loss of protective effect in the presence of capsazepine, a specific receptor antagonist of capsaicin [3].
• Further, the incorporation of apigenin, 3-hydroxyflavone, luteolin, and quercetin, which are more toxic, into TIG-1 cells during 24-h incubation was examined [13].
• Using 2',7'-dichlorofluorescin diacetate (DCF-DA), the intracellular reactive oxygen species (ROS) level of flavonoid-treated TIG-1 cells was examined [13].

Physical interactions of PCQAP

• TRIM11 binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105) through the ubiquitin-proteasome system [7].
• In a recent letter, Yang et al. present a comprehensive series of experiments, spanning a wide range of disciplines, that identify ARC105 as a component of the ARC complex that interacts directly with SREBP and is necessary for SREBP function () [14].

Other interactions of PCQAP

• PCQAP encodes for a subunit of the large multiprotein complex PC2, which exhibits a coactivator function in RNA polymerase II mediated transcription [15].
• Co-expression of TRIM11 increased ARC105 degradation but a proteasome inhibitor suppressed this [7].
• Although levels of hRPL39 mRNA were different in several cell lines including carcinoma cell lines from different tissues, they were shown not to be cell cycle-dependent in a human fibroblast cell line of TIG-1 [16].

References