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Gene Review

SCFV  -  single-chain Fv fragment

Homo sapiens

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Disease relevance of SCFV


Psychiatry related information on SCFV

  • We now report the isolation by phage display of human anti-idiotype (Id) monoclonal antibody fragments as single-chain Fv fragment (scFv) against anti-P antibodies [6].

High impact information on SCFV


Biological context of SCFV


Anatomical context of SCFV

  • High thermal stability is essential for tumor targeting of antibody fragments: engineering of a humanized anti-epithelial glycoprotein-2 (epithelial cell adhesion molecule) single-chain Fv fragment [16].
  • We have prepared a single-chain Fv fragment (scFv) derived from Y13-259, and here, we show that intracellular expression of the scFv led to the specific inhibition of the Ras signaling pathway in Xenopus laevis oocytes and NIH3T3 fibroblasts [17].
  • The mAb 5B-12B7 single chain Fv fragment bound to NS5B both in vitro and in transfected human cell lines and therefore may be potentially useful for intracellular immunization against HCV [2].
  • The single chain Fv fragment of mAb198 (scFv198) directed against the main immunogenic region (MIR) of the nicotinic acetylcholine receptor (AChR), can efficiently protect the AChR in muscle cell cultures against the destructive activity of human myasthenic autoantibodies [18].
  • As a first step in the creation of cytotoxic immunoconjugates, we constructed a single-chain Fv fragment derived from the murine hybridoma OKT3, that recognizes an epitope on the epsilon-subunit of the human CD3 complex [19].

Associations of SCFV with chemical compounds


Regulatory relationships of SCFV


Other interactions of SCFV


Analytical, diagnostic and therapeutic context of SCFV


  1. Site-specific chemical modification with polyethylene glycol of recombinant immunotoxin anti-Tac(Fv)-PE38 (LMB-2) improves antitumor activity and reduces animal toxicity and immunogenicity. Tsutsumi, Y., Onda, M., Nagata, S., Lee, B., Kreitman, R.J., Pastan, I. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  2. Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase. Moradpour, D., Bieck, E., Hügle, T., Wels, W., Wu, J.Z., Hong, Z., Blum, H.E., Bartenschlager, R. J. Biol. Chem. (2002) [Pubmed]
  3. Targeting the ATF-1/CREB transcription factors by single chain Fv fragment in human melanoma: potential modality for cancer therapy. Jean, D., Bar-Eli, M. Crit. Rev. Immunol. (2001) [Pubmed]
  4. Crystal structure of a phage library-derived single-chain Fv fragment complexed with turkey egg-white lysozyme at 2.0 A resolution. Aÿ, J., Keitel, T., Küttner, G., Wessner, H., Scholz, C., Hahn, M., Höhne, W. J. Mol. Biol. (2000) [Pubmed]
  5. Generation of a highly stable, internalizing anti-CD22 single-chain Fv fragment for targeting non-Hodgkin's lymphoma. Arndt, M.A., Krauss, J., Schwarzenbacher, R., Vu, B.K., Greene, S., Rybak, S.M. Int. J. Cancer (2003) [Pubmed]
  6. Production and characterization of a human monoclonal anti-idiotype to anti-ribosomal P antibodies. Zhang, W., Reichlin, M. Clin. Immunol. (2005) [Pubmed]
  7. Antigen binding forces of individually addressed single-chain Fv antibody molecules. Ros, R., Schwesinger, F., Anselmetti, D., Kubon, M., Schäfer, R., Plückthun, A., Tiefenauer, L. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  8. Integrated src kinase and costimulatory activity enhances signal transduction through single-chain chimeric receptors in T lymphocytes. Geiger, T.L., Nguyen, P., Leitenberg, D., Flavell, R.A. Blood (2001) [Pubmed]
  9. Pharmacokinetic and thrombolytic properties of chimeric plasminogen activators consisting of a single-chain Fv fragment of a fibrin-specific antibody fused to single-chain urokinase. Holvoet, P., Laroche, Y., Stassen, J.M., Lijnen, H.R., Van Hoef, B., De Cock, F., Van Houtven, A., Gansemans, Y., Matthyssens, G., Collen, D. Blood (1993) [Pubmed]
  10. C-type lectin-like molecule-1: a novel myeloid cell surface marker associated with acute myeloid leukemia. Bakker, A.B., van den Oudenrijn, S., Bakker, A.Q., Feller, N., van Meijer, M., Bia, J.A., Jongeneelen, M.A., Visser, T.J., Bijl, N., Geuijen, C.A., Marissen, W.E., Radosevic, K., Throsby, M., Schuurhuis, G.J., Ossenkoppele, G.J., de Kruif, J., Goudsmit, J., Kruisbeek, A.M. Cancer Res. (2004) [Pubmed]
  11. Selection for improved protein stability by phage display. Jung, S., Honegger, A., Plückthun, A. J. Mol. Biol. (1999) [Pubmed]
  12. 124I-labeled engineered anti-CEA minibodies and diabodies allow high-contrast, antigen-specific small-animal PET imaging of xenografts in athymic mice. Sundaresan, G., Yazaki, P.J., Shively, J.E., Finn, R.D., Larson, S.M., Raubitschek, A.A., Williams, L.E., Chatziioannou, A.F., Gambhir, S.S., Wu, A.M. J. Nucl. Med. (2003) [Pubmed]
  13. Bacterial expression of a single-chain Fv fragment which efficiently protects the acetylcholine receptor against antigenic modulation caused by myasthenic antibodies. Mamalaki, A., Trakas, N., Tzartos, S.J. Eur. J. Immunol. (1993) [Pubmed]
  14. Sequence analysis of a monoclonal antibody specific for the preS2 region of hepatitis B surface antigen, and the cloning, expression and characterisation of its single-chain Fv construction. Passafiume, M., Vulliez-le Normand, B., Riottot, M.M., Bentley, G.A. FEBS Lett. (1998) [Pubmed]
  15. A bivalent single-chain Fv fragment against CD47 induces apoptosis for leukemic cells. Kikuchi, Y., Uno, S., Yoshimura, Y., Otabe, K., Iida, S., Oheda, M., Fukushima, N., Tsuchiya, M. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
  16. High thermal stability is essential for tumor targeting of antibody fragments: engineering of a humanized anti-epithelial glycoprotein-2 (epithelial cell adhesion molecule) single-chain Fv fragment. Willuda, J., Honegger, A., Waibel, R., Schubiger, P.A., Stahel, R., Zangemeister-Wittke, U., Plückthun, A. Cancer Res. (1999) [Pubmed]
  17. Intracellular expression of an antibody fragment-neutralizing p21 ras promotes tumor regression. Cochet, O., Kenigsberg, M., Delumeau, I., Virone-Oddos, A., Multon, M.C., Fridman, W.H., Schweighoffer, F., Teillaud, J.L., Tocqué, B. Cancer Res. (1998) [Pubmed]
  18. Construction and characterization of a humanized single chain Fv antibody fragment against the main immunogenic region of the acetylcholine receptor. Papanastasiou, D., Mamalaki, A., Eliopoulos, E., Poulas, K., Liolitsas, C., Tzartos, S.J. J. Neuroimmunol. (1999) [Pubmed]
  19. Two amino acid mutations in an anti-human CD3 single chain Fv antibody fragment that affect the yield on bacterial secretion but not the affinity. Kipriyanov, S.M., Moldenhauer, G., Martin, A.C., Kupriyanova, O.A., Little, M. Protein Eng. (1997) [Pubmed]
  20. Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment of an antibody in solution investigated by heteronuclear three-dimensional NMR spectroscopy. Freund, C., Ross, A., Plückthun, A., Holak, T.A. Biochemistry (1994) [Pubmed]
  21. Structure of a glycoconjugate in solution and in complex with an antibody Fv fragment. Low, D.G., Probert, M.A., Embleton, G., Seshadri, K., Field, R.A., Homans, S.W., Windust, J., Davis, P.J. Glycobiology (1997) [Pubmed]
  22. The dipeptide, gamma-glutamylcysteine, is recognized by the anti-glutathione antibody single chain Fv fragment 20C9. Horibe, T., Furuya, R., Iwai, A., Yosho, C., Tujimoto, Y., Kikuchi, M. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  23. In vitro and in vivo inhibition of complement activity by a single-chain Fv fragment recognizing human C5. Evans, M.J., Rollins, S.A., Wolff, D.W., Rother, R.P., Norin, A.J., Therrien, D.M., Grijalva, G.A., Mueller, J.P., Nye, S.H., Squinto, S.P. Mol. Immunol. (1995) [Pubmed]
  24. Molecular simulation studies of a selenium-containing scFv catalytic antibody that mimics glutathione peroxidase. Zhang, Y., Li, Z.S., Sun, M., Zheng, Q.C., Sun, C.C. Biochim. Biophys. Acta (2005) [Pubmed]
  25. Inhibition of activating transcription factor 1- and cAMP-responsive element-binding protein-activated transcription by an intracellular single chain Fv fragment. Bosilevac, J.M., Gilchrist, C.A., Jankowski, P.E., Paul, S., Rees, A.R., Hinrichs, S.H. J. Biol. Chem. (1998) [Pubmed]
  26. Characterization of a chimeric plasminogen activator consisting of a single-chain Fv fragment derived from a fibrin fragment D-dimer-specific antibody and a truncated single-chain urokinase. Holvoet, P., Laroche, Y., Lijnen, H.R., Van Cauwenberge, R., Demarsin, E., Brouwers, E., Matthyssens, G., Collen, D. J. Biol. Chem. (1991) [Pubmed]
  27. Construction and characterisation of a functional CD19 specific single chain Fv fragment for immunotherapy of B lineage leukaemia and lymphoma. Nicholson, I.C., Lenton, K.A., Little, D.J., Decorso, T., Lee, F.T., Scott, A.M., Zola, H., Hohmann, A.W. Mol. Immunol. (1997) [Pubmed]
  28. Cloning and expression of a single-chain Fv fragment specific for the human interleukin 18 receptor. Nishida, Y., Torigoe, K., Aizawa, Y., Okura, T., Mori, T., Yamauchi, H., Tanimoto, T., Ikeda, M., Ikegami, H., Kurimoto, M. Hybridoma (1998) [Pubmed]
  29. The first constant domain (C(H)1 and C(L)) of an antibody used as heterodimerization domain for bispecific miniantibodies. Müller, K.M., Arndt, K.M., Strittmatter, W., Plückthun, A. FEBS Lett. (1998) [Pubmed]
  30. Antibody-mediated Hsp70 protein therapy. Hansen, J.E., Sohn, W., Kim, C., Chang, S.S., Huang, N.C., Santos, D.G., Chan, G., Weisbart, R.H., Nishimura, R.N. Brain Res. (2006) [Pubmed]
  31. Tumor targeting of mono-, di-, and tetravalent anti-p185(HER-2) miniantibodies multimerized by self-associating peptides. Willuda, J., Kubetzko, S., Waibel, R., Schubiger, P.A., Zangemeister-Wittke, U., Plückthun, A. J. Biol. Chem. (2001) [Pubmed]
  32. Production of a functional single-chain Fv fragment from the pan-leukocyte antibody WM65 using splicing by asymmetric PCR. Ward, R.L., Hawkins, N.J., Wakefield, D., Atkinson, K., Biggs, J.C. Exp. Hematol. (1993) [Pubmed]
  33. Production and characterization of a bacterial single-chain Fv fragment specific to human truncated midkine. Dansithong, W., Paul, S., Mitsumoto, T., Saruhashi, S., Shinozawa, T. Cancer Lett. (2001) [Pubmed]
  34. Noninvasive localization of tumors by immunofluorescence imaging using a single chain Fv fragment of a human monoclonal antibody with broad cancer specificity. Ramjiawan, B., Maiti, P., Aftanas, A., Kaplan, H., Fast, D., Mantsch, H.H., Jackson, M. Cancer (2000) [Pubmed]
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