Disease relevance of PCNA

• The EB can inhibit the proliferation and expression of PCNA in PASMC under hypoxia through PKC-signal way [1].
• METHOD: Animal models of gastritis associated with Hp infection were established using "Chinese No.1 pigs". The expressions of proliferating cell nuclear antigen (PCNA) and DNA polyploid content in porcine gastric epithelial cells were quantitatively assayed by means of immunohistochemistry and Feulgen stain respectively [2].
• At 7 days, proliferating cell nuclear antigen and retinoblastoma protein expression were reduced 60% and 50%, respectively, by the SRL stents [3].

High impact information on PCNA

• Cells traversing the cell cycle were identified by immunostaining for proliferating cell nuclear antigen (PCNA) [4].
• SMC proliferative activity was significantly lower in ethanol-treated arteries than in untreated arteries at 4 and 8 days after injury by BrdU and PCNA staining [5].
• In the third group (n=15), animals were killed at days 4, 8, and 14 after balloon injury, and coronary artery segments were studied by immunohistochemical staining against proliferating cell nuclear antigen (PCNA) and bromodeoxyuridine (BrdU) [5].
• At 28 days, there was no difference in smooth muscle cell proliferation as measured by the proliferating cell nuclear antigen index [6].
• Cell proliferation, as assessed by proliferating cell nuclear antigen immunostaining, reached the maximum level in the adventitia at 3 days, whereas at 14 and 28 days, the number of replicating cells reverted toward the baseline [7].

Biological context of PCNA

• The histologic degree of vessel injury, lesion morphometry and smooth muscle cell proliferation measured by immunolocalization with a monoclonal antibody to proliferating cell nuclear antigen (PCNA) were assessed at 24 h and 7, 14 and 28 days after stent placement [8].
• PGE(2) also elevated PCNA expression, bromodeoxyuridine incorporation, and cell number, indicative of a hyperplastic growth response [9].
• Cellular proliferation, apoptosis and PKA contents were evaluated by immunocytochemistry using specific antibodies against PCNA, TUNEL and catalytic (C-alpha) and regulatory (RI) subunits of PKA [10].
• PCNA, a nuclear protein essential for cell division, identifies recently divided cells in the brains of healthy animals [11].
• TK, PCNA, and gut weight showed an energy effect on mitosis, which was paralleled by increased peripheral IGF-I [12].

Anatomical context of PCNA

• Scattered cells labelled with anti-BrdUrd or anti-PCNA antibodies were detected within the ES whereas labelled cells were not found in either the cochlea or the vestibule [13].
• These findings were associated with increased frequency of hepatocytes with apoptotic bodies and positivity for proliferating cell nuclear antigen (PCNA) in livers from ethanol-fed minipigs [14].
• PCNA staining of 1-week vein grafts confirmed in vivo anti-proliferative effects of paclitaxel (21+/-2 vs. 36+/-3%, n=5, p<0.01) and rapamycin (32+/-1 vs. 57+/-6%, n=6, p<0.005); neither agent stimulated loss of endothelium at these concentrations [15].
• PCNA staining was prominent within tubular primordia and was higher in testes from Yorkshire than from Meishan fetuses on all days examined [16].
• Abundance of IGF-I and PCNA mRNAs was increased by E and inhibited by coaddition of either T or N in Day 10 pregnant pig endometrium [17].

Associations of PCNA with chemical compounds

• PCNA labeling increased opposite to the injury site in both control and capsaicin animals over the first 72 h [18].
• To evaluate a role for AR in uterine gene regulation, the levels of mRNAs for insulin-like growth factor-I (IGF-I), proliferative cell nuclear antigen (PCNA), and AR itself were assessed in uterine endometrial explant cultures treated with estradiol-17beta (E), testosterone (T), and 19-nortestosterone (N) [17].
• In contrast, PCNA mRNA abundance was suppressed by all steroid treatments in these explants [17].
• In the presence of deferoxamine, [3H] methyl-thymidine incorporation, expression of nuclear proteins (p34cdc2 and PCNA) and H1 kinase activity were drastically reduced [19].
• RESULTS: at 24 h, PCNA-positive nuclei revealed 33+/-14 and 12+/-3 proliferating cells/mm2 at placebo and molsidomine PTA-LDD sites, respectively (p<0.001) [20].

Other interactions of PCNA

• The intraperitoneal injection of 1,000 ng bFGF/day for 14 days increased the cells in proliferation detected by immunostaining with proliferating cell nuclear antigen (PCNA), while neither EGF nor low-dose bFGF had any effect [21].
• Additional information about drug effects was obtained by immunoblotting to detect expression of proliferating cell nuclear antigen, p27(kip1) and the caspase-3 substrate poly(ADP-ribose) polymerase [22].
• Cell culture, immunocytochemical staining, image analysis and flow cytometric method were used to investigate the effects of HECCM and DDPH on the expression of alpha-smooth muscle actin (alpha-SM-Actin) antigen, CD34 antigen, S-100 antigen and proliferating cell nuclear antigen (PCNA) and cell cycle in pericytes [23].

Analytical, diagnostic and therapeutic context of PCNA

• In comparison, PCNA staining peaked at 3 days after PTCA (7.16+/-0.29%; compared to 1.78+/-0.08% PCNA-positive cells in the uninjured artery, P<0.001) [4].
• The expression of P450c17 was localized in fetal gonads by in situ hybridization and immunocytochemistry and related to cellular proliferation through expression of the proliferating cell nuclear antigen (PCNA) [16].
• The release of prostaglandins F (PGF) and E (PGE) were determined by RIA, PKA (alpha-cat subunit), the proliferation-associated peptide PCNA and ERK-1, -2 expression in cell lyzates were analysed by Western-blotting [24].
• In the 2 sham-control animals, there was a low index of PCNA-positive myocytes on both the osteotomy (2%, 5%) and the contralateral sides (1%, 2%) [25].
• RESULTS: The values of PCNA labeling index (LI) in the porcine gastric epithelial cells of the experimental group were significantly higher than those of the control group (40.95+/-3.60 vs 29.4+/-12.82, P<0.01) [2].

References