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Gene Review

Myh10  -  myosin, heavy polypeptide 10, non-muscle

Mus musculus

Synonyms: 5730504C04Rik, 9330167F11Rik, Cellular myosin heavy chain, type B, Fltn, Myhn-2, ...
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Disease relevance of Myh10

  • Approximately 65% of the NMHC-B-/- embryos died prior to birth, and those that were born suffered from congestive heart failure and died during the first day [1].
  • Preformed neurites in mouse Neuro-2A neuroblastoma cells undergo immediate retraction when exposed to isoform-specific antisense oligonucleotides that suppress myosin IIB expression, ruling out myosin IIB as the retraction motor [2].

High impact information on Myh10

  • These data on B(DeltaI)/B(DeltaI) and B(DeltaI)/B(-) mice demonstrate a gene dosage effect of the amount of NMHC-B on the severity and time of onset of the defects in the heart and brain [3].
  • Complete ablation of nonmuscle myosin heavy chain II-B (NMHC-B) in mice resulted in cardiac and brain defects that were lethal during embryonic development or on the day of birth [3].
  • By contrast, the myosin IIA isoform is shown to be expressed constitutively both before and during neurite outgrowth and throughout exposure to myosin IIB antisense oligodeoxyribonucleotides [4].
  • The increase in size of the cardiac myocytes was seen as early as embryonic day 12.5 (4.5 +/- 0.2 micron for NMHC-B+/+ and B+/- vs. 7. 2 +/- 0.6 micron for NMHC-B-/- mice (P < 0.01)) [1].
  • The absence of NMHC-B resulted in a significant increase in the transverse diameters of the cardiac myocytes from 7.8 +/- 1.8 micron (right ventricle) and 7.8 +/- 1.3 micron (left ventricle) in NMHC-B+/+ and B+/- mice to 14.7 +/- 1.1 micron and 13.8 +/- 2.3 micron, respectively, in NMHC-B-/- mice (in both cases, P < 0.001) [1].

Biological context of Myh10


Anatomical context of Myh10

  • NMHC II-C contains an alternatively spliced exon of 24 nucleotides in loop I at a location analogous to where a spliced exon appears in NMHC II-B and in the smooth muscle myosin heavy chain [5].
  • In contrast to most cells, cardiac myocytes lack NMHC II-A, and ablation of NMHC II-B results in a heart with 70% fewer myocytes at embryonic day 14.5 (E14.5) than control mice (B+/B- and B+/B+) [6].
  • Six of seven NMHC-B-/- newborn mice analyzed by serial sectioning also showed structural cardiac defects, including a ventricular septal defect, an aortic root that either straddled the defect or originated from the right ventricle, and muscular obstruction to right ventricular outflow [1].
  • The interaction of NMHCB with PBX was verified by coimmunoprecipitation, and immunofluorescence staining revealed colocalization of NMHCB with cytoplasmic PBX in the mouse embryo distal limb bud [8].
  • Nonmuscle myosin IIb is involved in the guidance of fibroblast migration [9].

Associations of Myh10 with chemical compounds

  • Myosin IIB null cells displayed multiple unstable and disorganized protrusions, although they were still able to generate a large fraction of traction forces when cultured on flexible polyacrylamide substrates [9].
  • In contrast, Y27632 did not inhibit outgrowth, a myosin IIB-dependent process [2].
  • The clonal transfectants, expressing muristerone A-induced nmMHC-B, displayed a slightly flatter morphology and reached to a lower saturation density compared to the parental transformed cells [10].

Physical interactions of Myh10


Regulatory relationships of Myh10


Other interactions of Myh10


Analytical, diagnostic and therapeutic context of Myh10


  1. Nonmuscle myosin II-B is required for normal development of the mouse heart. Tullio, A.N., Accili, D., Ferrans, V.J., Yu, Z.X., Takeda, K., Grinberg, A., Westphal, H., Preston, Y.A., Adelstein, R.S. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  2. Myosin IIA drives neurite retraction. Wylie, S.R., Chantler, P.D. Mol. Biol. Cell (2003) [Pubmed]
  3. Gene dosage affects the cardiac and brain phenotype in nonmuscle myosin II-B-depleted mice. Uren, D., Hwang, H.K., Hara, Y., Takeda, K., Kawamoto, S., Tullio, A.N., Yu, Z.X., Ferrans, V.J., Tresser, N., Grinberg, A., Preston, Y.A., Adelstein, R.S. J. Clin. Invest. (2000) [Pubmed]
  4. A conventional myosin motor drives neurite outgrowth. Wylie, S.R., Wu, P.J., Patel, H., Chantler, P.D. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  5. Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family. Golomb, E., Ma, X., Jana, S.S., Preston, Y.A., Kawamoto, S., Shoham, N.G., Goldin, E., Conti, M.A., Sellers, J.R., Adelstein, R.S. J. Biol. Chem. (2004) [Pubmed]
  6. Ablation and mutation of nonmuscle myosin heavy chain II-B results in a defect in cardiac myocyte cytokinesis. Takeda, K., Kishi, H., Ma, X., Yu, Z.X., Adelstein, R.S. Circ. Res. (2003) [Pubmed]
  7. Nonmuscle myosin IIA and IIB have distinct functions in the exocytosis-dependent process of cell membrane repair. Togo, T., Steinhardt, R.A. Mol. Biol. Cell (2004) [Pubmed]
  8. Nonmuscle myosin promotes cytoplasmic localization of PBX. Huang, H., Paliouras, M., Rambaldi, I., Lasko, P., Featherstone, M. Mol. Cell. Biol. (2003) [Pubmed]
  9. Nonmuscle myosin IIb is involved in the guidance of fibroblast migration. Lo, C.M., Buxton, D.B., Chua, G.C., Dembo, M., Adelstein, R.S., Wang, Y.L. Mol. Biol. Cell (2004) [Pubmed]
  10. Suppression of the tumorigenicity of mutant p53-transformed rat embryo fibroblasts through expression of a newly cloned rat nonmuscle myosin heavy chain-B. Yam, J.W., Chan, K.W., Hsiao, W.L. Oncogene (2001) [Pubmed]
  11. Non-muscle myosin IIB-like immunoreactivity is present at the drebrin-binding cytoskeleton in neurons. Cheng, X.T., Hayashi, K., Shirao, T. Neurosci. Res. (2000) [Pubmed]
  12. Retrograde flow rate is increased in growth cones from myosin IIB knockout mice. Brown, M.E., Bridgman, P.C. J. Cell. Sci. (2003) [Pubmed]
  13. Actomyosin-dependent microtubule rearrangement in lysophosphatidic acid-induced neurite remodeling of young cortical neurons. Fukushima, N., Morita, Y. Brain Res. (2006) [Pubmed]
  14. Homeobox protein Hex induces SMemb/nonmuscle myosin heavy chain-B gene expression through the cAMP-responsive element. Sekiguchi, K., Kurabayashi, M., Oyama, Y., Aihara, Y., Tanaka, T., Sakamoto, H., Hoshino, Y., Kanda, T., Yokoyama, T., Shimomura, Y., Iijima, H., Ohyama, Y., Nagai, R. Circ. Res. (2001) [Pubmed]
  15. Structural abnormalities develop in the brain after ablation of the gene encoding nonmuscle myosin II-B heavy chain. Tullio, A.N., Bridgman, P.C., Tresser, N.J., Chan, C.C., Conti, M.A., Adelstein, R.S., Hara, Y. J. Comp. Neurol. (2001) [Pubmed]
  16. Oxidative stress produced with cell migration increases synthetic phenotype of vascular smooth muscle cells. Sung, H.J., Eskin, S.G., Sakurai, Y., Yee, A., Kataoka, N., McIntire, L.V. Annals of biomedical engineering. (2005) [Pubmed]
  17. Differential expression and functions of cortical myosin IIA and IIB isotypes during meiotic maturation, fertilization, and mitosis in mouse oocytes and embryos. Simerly, C., Nowak, G., de Lanerolle, P., Schatten, G. Mol. Biol. Cell (1998) [Pubmed]
  18. Nonmuscle myosins IIA and IIB are present in adult motor nerve terminals. Vega-Riveroll, L.J., Wylie, S.R., Loughna, P.T., Parson, S.H., Chantler, P.D. Neuroreport (2005) [Pubmed]
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