Disease relevance of rpsG

• A striking difference is that the rpsG-encoded ribosomal protein, S7, in E. coli K-12 is 23 aa longer than in S. typhimurium [1].
• Although the two phage proteins differ in their chromatographic and antigenic properties, they act by the same mechanism: the anti-restriction proteins inhibit E. coli K12 restriction endonuclease by direct interaction [2].
• These findings support the previous conclusions, based on infectivity assays in mice, that propagation of polyoma virus DNA as a component of recombinant DNA molecules in E. coli K12 reduces its biologic activity many orders of magnitude relative to the virus itself [3].
• Dead intimin beta+ C. rodentium, intimin alpha-transfected C. rodentium or E. coli strain K12, and EPEC induced mucosal hyperplasia identical to that caused by C. rodentium live infection, as well as a massive T helper cell-type 1 immune response in the colonic mucosa [4].
• Structural homologies among different restriction systems of Escherichia coli and several Salmonella species have been investigated by immunological methods using antibodies prepared against two subunits of the E. coli K12 restriction enzyme, and by DNA hybridization experiments using different fragments of the E. coli K12 hsd genes as probes [5].

High impact information on rpsG

• The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12 [6].
• The structures and fidelity of replication of mouse mitochondrial DNA-pSC 101 EcoRI recombinant plasmids grown in E. coli K12 [7].
• Introns in the chicken ovalbumin gene prevent ovalbumin synthesis in E. coli K12 [8].
• Cloning of replication origins from the E. coli K12 chromosome [9].
• Distribution of inverted IS-length sequences in the E. coli K12 genome [10].

Chemical compound and disease context of rpsG

• Isolation and characterization of mutants of Escherichia coli K12 resistant to the new aminoglycoside antibiotic, amikacin [11].
• Strains of Escherichia coli K12 have been constructed as safer hosts for use in recombinant DNA research, These strains are unable to survive passage through the intestinal tracts of rats because of a constellation of mutations conferring bile sensitivity and requirements for diaminopimelic acid and thymine [12].
• The fourth strain was resistant to tobramycin; its gentamicin/tobramycin resistance was transferable to E. coli K12 [13].
• Promotion of RNA transcription on the insertion element IS30 of E. coli K12 [14].
• We have isolated several classes of spontaneous mutants resistant to the calmodulin inhibitor 48/80 which inhibits cell division in Escherichia coli K12 [15].

Biological context of rpsG

• The nucleotide (nt) sequences of the str operon in Escherichia coli K-12 and Salmonella typhimurium LT2 were completed and compared at the nt and amino acid (aa) level [1].
• The complete genome sequence of Escherichia coli K-12 [16].
• Availability of the complete genome sequence of two K-12 strains allows comparison of their genotypes and mutant status of alleles [17].
• The fourth plasmid, a 6.2 kb resistance element carrying sul2, strA and strB designated p9123, conferred a 4% fitness advantage upon its original clinical host and also on E. coli K12 JM109 [18].
• The complete nucleotide sequence of the mobile genetic element IS30, a resident of Escherichia coli K12, is 1221 bp long [19].

Anatomical context of rpsG

• Integration into the cytoplasmic membrane and function of the three F0 subunits, a, b and c, of the membrane-bound ATP synthase of Escherichia coli K12 were analysed in situations where synthesis of only one or two types of subunits was possible [20].
• The anti-acpP PNA at 2 microM concentration cured HeLa cell cultures noninvasively infected with E. coli K12 without any apparent toxicity to the human cells [21].
• E. coli K-12 strain KY895 cells transformed by pMH1A did not show enhanced incorporation of [3H]dThd into bacterial DNA, although pMH1A DNA isolated from transformed KY895 cells, like pAGO DNA, did transform TK- mouse fibroblast [LM(TK-)] cells to the TK+ phenotype [22].
• We have used nylon membranes spotted in duplicate with full-length polymerase chain reaction-generated products of each of the 4,290 predicted Escherichia coli K12 open reading frames (ORFs) to measure the gene expression profiles in otherwise isogenic integration host factor IHF(+) and IHF(-) strains [23].
• Cloned diphtheria toxin fragment A is expressed from the tox promoter and exported to the periplasm by the SecA apparatus of Escherichia coli K12 [24].

Associations of rpsG with chemical compounds

• RF2 from E.coli K12 is unique in having Thr246 near the GGQ motif, where all other sequenced bacterial class 1 RFs have alanine or serine [25].
• The activity of unmethylated RF2 from K12 strains is extremely low due to the cumulative effects of threonine at position 246, in place of alanine or serine present in all other bacterial RFs, and the lack of N(5)-methylation of Gln252 [26].
• We propose that the previously observed lethal effect of overproducing E.coli K12 RF2 arises through accumulating the defects due to lack of Gln252 methylation and Thr246 in place of alanine [25].
• In environments that cycle between high and low arginine, short seasons favor the K12 allele, whereas long seasons favor the B allele [27].
• Bacteriol. 117, 1093-1098) and strain K12 CS (Barash, H., and Halpern, Y.S. (1971) Biochem. Biophys. Res. Commun. 45, 681-688) [28].

Other interactions of rpsG

• Temperature-sensitive (ts) mutations were isolated within a ribosomal protein gene (rpsL) of Escherichia coli K12 [29].

Analytical, diagnostic and therapeutic context of rpsG

• Recombinant DNAs containing the E. coli plasmid pSC101 and mouse cell (La9) mitochondrial DNA (mtDNA) were formed in vitro via ligation of DNA fragments from limit EcoRI endonuclease digests and were used to transform E. coli K12 [7].
• Improved statistical inference from DNA microarray data using analysis of variance and a Bayesian statistical framework. Analysis of global gene expression in Escherichia coli K12 [30].
• The colicin I outer membrane receptor was solubilized from the cell envelope of Escherichia coli K12 by extraction with Triton X-100 and purified to homogeneity by a combination of ion exchange and gel filtration chromatography as well as isoelectric focusing [31].
• Immunoprecipitation experiments revealed that the mature form of the fragment A-related polypeptide was exported to the periplasmic compartment of E. coli K12 harboring plasmid pDT201 [24].
• We have characterized lpcA as a single gene mapping around 5.3 min (246 kilobases) on the E. coli K12 chromosome and encoding a 22.6-kDa cytosolic protein [32].

References