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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Protein Denaturation

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Disease relevance of Protein Denaturation


High impact information on Protein Denaturation


Biological context of Protein Denaturation


Anatomical context of Protein Denaturation


Associations of Protein Denaturation with chemical compounds

  • The antitumor agent 4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA) inhibits topoisomerase II activity through the formation of a complex of DNA and covalently bound enzyme which, upon protein denaturation, yields DNA breaks (single strand breaks) [15].
  • In this paper, we observe that pretreatment of cells with D2O and glycerol, compounds known to stabilize protein structure, leads to a parallel decrease of protein inactivation and insolubilization, suggesting that these two phenomena result most probably from heat-induced protein denaturation [16].
  • 5. There is a decrease in the heat capacity of protein denaturation (DeltaCp) in trehalose solutions for all the studied proteins [17].
  • Protein denaturation may also have caused the increased proteolytic susceptibility induced by .OH + O2- + O2, but no simple correlation could be obtained [18].
  • Treatment of modified enzyme with KCN or thiols results in a complete restoration of enzyme activity demonstrating that the inactivation is not due to irreversible protein denaturation [19].

Gene context of Protein Denaturation

  • Activity of ADAMTS-13 is usually measured in a static system under non-physiological conditions that require protein denaturation and prolonged incubation [20].
  • The resultant Hsp70 mutants directly interacted with active caspase-3 and blocked its proteolytic activity while retaining the ability to reverse protein denaturation and disrupt the interaction between Apaf-1 and procaspase-9 [21].
  • These results suggest that the expression of Hsp70, mostly Hsc70, increases with aging and may have a role to suppress protein denaturation [22].
  • The protective effect of LC1 was abolished by protein denaturation and not mimicked by the structurally related protein annexin V [23].
  • Analysis of the polarization-dependent SHG generated during BSA adsorption at glass/aqueous solution interfaces provided direct evidence for slow conformational changes within the protein layer after adsorption, consistent with protein denaturation [24].

Analytical, diagnostic and therapeutic context of Protein Denaturation


  1. Thermal analysis of CHL V79 cells using differential scanning calorimetry: implications for hyperthermic cell killing and the heat shock response. Lepock, J.R., Frey, H.E., Rodahl, A.M., Kruuv, J. J. Cell. Physiol. (1988) [Pubmed]
  2. Improved "electrophoretic map" of breast cyst fluid proteins. Troccoli, R., Mannello, F. Cancer Detect. Prev. (1992) [Pubmed]
  3. Extrinsic tooth discoloration by metals and chlorhexidine. I. Surface protein denaturation or dietary precipitation? Addy, M., Moran, J., Griffiths, A.A., Wills-Wood, N.J. British dental journal. (1985) [Pubmed]
  4. Identification of thermolabile Escherichia coli proteins: prevention and reversion of aggregation by DnaK and ClpB. Mogk, A., Tomoyasu, T., Goloubinoff, P., Rüdiger, S., Röder, D., Langen, H., Bukau, B. EMBO J. (1999) [Pubmed]
  5. Counteraction of urea-induced protein denaturation by trimethylamine N-oxide: a chemical chaperone at atomic resolution. Bennion, B.J., Daggett, V. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  6. Heat shock protein coinducers with no effect on protein denaturation specifically modulate the membrane lipid phase. Török, Z., Tsvetkova, N.M., Balogh, G., Horváth, I., Nagy, E., Pénzes, Z., Hargitai, J., Bensaude, O., Csermely, P., Crowe, J.H., Maresca, B., Vigh, L. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  7. Creutzfeldt-Jakob disease: Carnoy's fixative improves the immunohistochemistry of the proteinase K-resistant prion protein. Giaccone, G., Canciani, B., Puoti, G., Rossi, G., Goffredo, D., Iussich, S., Fociani, P., Tagliavini, F., Bugiani, O. Brain Pathol. (2000) [Pubmed]
  8. Stress-induced inhibition of the NF-kappaB signaling pathway results from the insolubilization of the IkappaB kinase complex following its dissociation from heat shock protein 90. Pittet, J.F., Lee, H., Pespeni, M., O'Mahony, A., Roux, J., Welch, W.J. J. Immunol. (2005) [Pubmed]
  9. Contribution to global protein stabilization of the N-capping box in human growth hormone. Zhukovsky, E.A., Mulkerrin, M.G., Presta, L.G. Biochemistry (1994) [Pubmed]
  10. Injury of the cell's respiratory system by heat and by formaldehyde. Thermokinetics and early molecular events. Johnson, H.A., Wiske, P.S. Lab. Invest. (1976) [Pubmed]
  11. Protein profile of aging and its retardation by caloric restriction in neural retina. Li, D., Sun, F., Wang, K. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
  12. Complications of percutaneous ethanol ablation. Gelczer, R.K., Charboneau, J.W., Hussain, S., Brown, D.L. Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine. (1998) [Pubmed]
  13. Protein stability and interaction of the nicotinic acetylcholine receptor with cholinergic ligands studied by Fourier-transform infrared spectroscopy. Fernandez-Ballester, G., Castresana, J., Arrondo, J.L., Ferragut, J.A., Gonzalez-Ros, J.M. Biochem. J. (1992) [Pubmed]
  14. Bacterial production and purification of recombinant human prolactin. Paris, N., Rentier-Delrue, F., Defontaine, A., Goffin, V., Lebrun, J.J., Mercier, L., Martial, J.A. Biotechnol. Appl. Biochem. (1990) [Pubmed]
  15. Comparison of DNA-protein cross-links induced by 4'-(9-acridinylamino)-methanesulfon-m-anisidide and by gamma-radiation. Chiu, S.M., Xue, L.Y., Friedman, L.R., Oleinick, N.L. Cancer Res. (1989) [Pubmed]
  16. Denaturation of proteins during heat shock. In vivo recovery of solubility and activity of reporter enzymes. Pinto, M., Morange, M., Bensaude, O. J. Biol. Chem. (1991) [Pubmed]
  17. Why is trehalose an exceptional protein stabilizer? An analysis of the thermal stability of proteins in the presence of the compatible osmolyte trehalose. Kaushik, J.K., Bhat, R. J. Biol. Chem. (2003) [Pubmed]
  18. Protein damage and degradation by oxygen radicals. IV. Degradation of denatured protein. Davies, K.J., Lin, S.W., Pacifici, R.E. J. Biol. Chem. (1987) [Pubmed]
  19. Chemical modification of chalcone isomerase by mercurials and tetrathionate. Evidence for a single cysteine residue in the active site. Bednar, R.A., Fried, W.B., Lock, Y.W., Pramanik, B. J. Biol. Chem. (1989) [Pubmed]
  20. ADAMTS-13 metalloprotease interacts with the endothelial cell-derived ultra-large von Willebrand factor. Dong, J.F., Moake, J.L., Bernardo, A., Fujikawa, K., Ball, C., Nolasco, L., López, J.A., Cruz, M.A. J. Biol. Chem. (2003) [Pubmed]
  21. Hsp70 mutant proteins modulate additional apoptotic pathways and improve cell survival. Ran, R., Zhou, G., Lu, A., Zhang, L., Tang, Y., Rigby, A.C., Sharp, F.R. Cell Stress Chaperones (2004) [Pubmed]
  22. Increase in basal level of Hsp70, consisting chiefly of constitutively expressed Hsp70 (Hsc70) in aged rat brain. Unno, K., Asakura, H., Shibuya, Y., Kaiho, M., Okada, S., Oku, N. J. Gerontol. A Biol. Sci. Med. Sci. (2000) [Pubmed]
  23. Lipocortin 1 reduces myocardial ischemia-reperfusion injury by affecting local leukocyte recruitment. D'Amico, M., Di Filippo, C., La, M., Solito, E., McLean, P.G., Flower, R.J., Oliani, S.M., Perretti, M. FASEB J. (2000) [Pubmed]
  24. Ellipsometric approach for the real-time detection of label-free protein adsorption by second harmonic generation. Polizzi, M.A., Plocinik, R.M., Simpson, G.J. J. Am. Chem. Soc. (2004) [Pubmed]
  25. A new protein antidenaturant agent, bindarit, reduces secondary phase of adjuvant arthritis in rats. Cioli, V., Ciarniello, M.G., Guglielmotti, A., Luparini, M.R., Durando, L., Martinelli, B., Catanese, B., Fava, L., Silvestrini, B. J. Rheumatol. (1992) [Pubmed]
  26. Cycloheximide increases the thermostability of proteins in Chinese hamster ovary cells. Borrelli, M.J., Lee, Y.J., Frey, H.E., Ofenstein, J.P., Lepock, J.R. Biochem. Biophys. Res. Commun. (1991) [Pubmed]
  27. Ethylene oxide sterilization impairs allograft incorporation in a conduction chamber. Thorén, K., Aspenberg, P. Clin. Orthop. Relat. Res. (1995) [Pubmed]
  28. Stabilization of dichloromethane-induced protein denaturation during microencapsulation. Raghuvanshi, R.S., Goyal, S., Singh, O., Panda, A.K. Pharmaceutical development and technology. (1998) [Pubmed]
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