Disease relevance of Protein Denaturation

• Glycerol, a hyperthermic protector, non-specifically raises the denaturation temperature for proteins denaturing below 60 degrees C. Thus, there is a correlation between the effect of these compounds on protein denaturation in vivo and their effect on cellular sensitivity to hyperthermia [1].
• Human breast cyst fluid (BCF) proteins were evaluated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) after protein denaturation in the presence or absence of 2-mercaptoethanol [2].
• Extrinsic tooth discoloration by metals and chlorhexidine. I. Surface protein denaturation or dietary precipitation [3]?

High impact information on Protein Denaturation

• Keywords: chaperones/heat-shock response/Hsp70/protein denaturation/thermotolerance[4]
• Counteraction of urea-induced protein denaturation by trimethylamine N-oxide: a chemical chaperone at atomic resolution [5].
• Heat shock protein coinducers with no effect on protein denaturation specifically modulate the membrane lipid phase [6].
• We show here that the presence of BM does not influence protein denaturation in the cells [6].
• Accordingly, Carnoy's-fixed tissue can prior to immunolabeling be subjected to proteinase K and guanidine thiocyanate, which respectively eliminate the normal cellular form of prion protein and promote protein denaturation [7].

Biological context of Protein Denaturation

• Pretreatment of cells with glycerol, a chemical chaperone that reduces the extent of stress-induced protein denaturation, reduced the stress-dependent detergent insolubility of the IKK complex and restored the cytokine-stimulated phosphorylation of IkappaB [8].
• Ultraviolet spectroscopy and near- and far-UV CD spectroscopy as well as guanidine hydrochloride protein denaturation were used as assays to monitor the conformational and free energy of stabilization changes induced by the point mutations [9].
• (3) Repression of cell respiration by diluted formaldehyde has an activation energy compatible with a chemical reaction but low enough to exclude protein denaturation as a mechanism [10].
• These data are the first to suggest that CR may retard the age-related degeneration of retina by maintaining sufficient glucose metabolism, by ensuring proper protein folding, and/or by preventing protein denaturation in the neural retina [11].
• Local infiltration or intravascular injection of ethanol leads to cell death by causing cell membrane lysis, protein denaturation, and vascular occlusion [12].

Anatomical context of Protein Denaturation

• The interference of water absorbance on the amide-I spectral region has been eliminated through isotopic exchange by freeze-drying the membranes in the presence of trehalose to avoid protein denaturation induced by drying, followed by resuspension in deuterated water [13].
• Solubilization of the inclusion bodies and protein denaturation were performed in 8 M urea [14].

Associations of Protein Denaturation with chemical compounds

• The antitumor agent 4'-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA) inhibits topoisomerase II activity through the formation of a complex of DNA and covalently bound enzyme which, upon protein denaturation, yields DNA breaks (single strand breaks) [15].
• In this paper, we observe that pretreatment of cells with D2O and glycerol, compounds known to stabilize protein structure, leads to a parallel decrease of protein inactivation and insolubilization, suggesting that these two phenomena result most probably from heat-induced protein denaturation [16].
• 5. There is a decrease in the heat capacity of protein denaturation (DeltaCp) in trehalose solutions for all the studied proteins [17].
• Protein denaturation may also have caused the increased proteolytic susceptibility induced by .OH + O2- + O2, but no simple correlation could be obtained [18].
• Treatment of modified enzyme with KCN or thiols results in a complete restoration of enzyme activity demonstrating that the inactivation is not due to irreversible protein denaturation [19].

Gene context of Protein Denaturation

• Activity of ADAMTS-13 is usually measured in a static system under non-physiological conditions that require protein denaturation and prolonged incubation [20].
• The resultant Hsp70 mutants directly interacted with active caspase-3 and blocked its proteolytic activity while retaining the ability to reverse protein denaturation and disrupt the interaction between Apaf-1 and procaspase-9 [21].
• These results suggest that the expression of Hsp70, mostly Hsc70, increases with aging and may have a role to suppress protein denaturation [22].
• The protective effect of LC1 was abolished by protein denaturation and not mimicked by the structurally related protein annexin V [23].
• Analysis of the polarization-dependent SHG generated during BSA adsorption at glass/aqueous solution interfaces provided direct evidence for slow conformational changes within the protein layer after adsorption, consistent with protein denaturation [24].

Analytical, diagnostic and therapeutic context of Protein Denaturation

• The involvement of protein denaturation in the production of new antigenic determinants, their pathogenic relevance in the development of adjuvant arthritis and the possibility that protein stabilization by bindarit could be the mechanism of action of the drug are discussed [25].
• To test this, Chinese hamster ovary cells were treated with the heat protector cycloheximide and then subjected to differential scanning calorimetry to measure protein denaturation [26].
• Bone allograft processing often includes treatment with hydrogen peroxide for protein denaturation and sterilization by irradiation or ethylene oxide [27].
• Stabilization of dichloromethane-induced protein denaturation during microencapsulation [28].

References