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Gene Review

ANXA5  -  annexin A5

Homo sapiens

Synonyms: ANX5, Anchorin CII, Annexin A5, Annexin V, Annexin-5, ...
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Disease relevance of ANXA5


Psychiatry related information on ANXA5

  • Time studies revealed that these cells went into apoptosis after prolonged incubation times up to 18 hours.Application of biotinilated annexin V for EM detection with gold-conjugated anti-biotin, showed that only clear-cut apoptotic, apoptotic necrotic and oncotic cells showed the gold-label at their membranes [5].
  • Annexin V concentrations of the CSF did not correlate with dementia, duration of symptoms, age, sex, or treatment of PD [6].
  • The radiolabelling efficiency of (124)I Annexin-V was 40% after 1 min reaction time [7].
  • To understand the mechanism of annexin V-membrane interactions, we measured the interaction of human recombinant annexin V with phospholipid monolayers with differing head group and acyl group structures [8].

High impact information on ANXA5


Chemical compound and disease context of ANXA5


Biological context of ANXA5


Anatomical context of ANXA5


Associations of ANXA5 with chemical compounds

  • Protein kinase C (PKC) blockade (PKC(19-36)) abolished the inhibitory effects of dexamethasone on forskolin-evoked peptide release and attenuated the antisecretory actions of ANXA1(Ac2-26.) ANXA5, which sequesters PKC in other systems, produced similar effects [21].
  • Endonexin II is a protein with similar Ca2+ and phospholipid binding properties as lipocortin 1, but it is not a substrate for tyrosine kinases [22].
  • Cell surface-expressed phosphatidylserine and annexin A5 open a novel portal of cell entry [16].
  • Endonexin II was not phosphorylated by Ca2+/phospholipid-dependent enzyme (protein kinase C) even though it contained a threonine at a position analogous to the protein kinase C phosphorylation sites of lipocortin I, calpactin I, and protein II [2].
  • Annexin V bound to heparin and heparan sulfate columns but not to chondroitin sulfate columns [23].

Physical interactions of ANXA5


Regulatory relationships of ANXA5


Other interactions of ANXA5

  • Annexin V is present in both cardiomyocytes and non-myocyte cell types in the heart and may play a role in the regulation of cellular ion fluxes, organization and secretion, while the cardiac effects of annexin II are unclear [33].
  • CONCLUSIONS: The results demonstrate expression of both ANXA1 and ANXA5 in human pituitary tumours and thus raise the possibility that these proteins influence the growth and/or functional activity of the tumours [1].
  • Annexin III or annexin V did not bind this receptor [34].
  • The 73-kDa protein is related to the 67-kDa calelectrin and to lipocortin VI; the 32-kDa protein is different from endonexin I but related to chromobindin 7 and to lipocortin V [35].
  • Only annexin IV and annexin V have unstructured cytoplasmic staining patterns consistent with predominantly cytosolic locations [36].

Analytical, diagnostic and therapeutic context of ANXA5

  • Sequence analysis of 70 German RPL patients, all known to carry neither factor V Leiden nor a prothrombin mutation, revealed four consecutive nucleotide substitutions in the ANXA5 promoter, which were transmitted as a joint haplotype (M2) [37].
  • Human annexin V (PP4), a member of the family of calcium, membrane binding proteins, has been crystallized in the presence of calcium and analysed by crystallography by multiple isomorphic replacement at 3 A and preliminarily refined at 2.5 A resolution [38].
  • On the basis of the array results, we characterized apoptosis of normal and ET MKs by time-course evaluation of annexin-V and sub-G1 peak DNA stainings of immature and mature MKs after culture in serum-free medium with an optimal thrombopoietin concentration, and annexin-V-positive MKs only, with decreasing thrombopoietin concentrations [39].
  • Characteristic features of apoptosis-like incorporation of propidium iodide (PI), such as increased annexin V binding, changes in mitochondrial membrane potential, and induction of DNA breaks (as evidenced by TUNEL techniques), could be induced in lymphoblasts after chlorpromazine treatment [40].
  • Chemotactic activity (modified Boyden chamber assay), eosinophil cationic protein release (fluoroenzyme immunoassay), respiratory burst (lucigenin-dependent chemiluminescence), and apoptosis (Nicoletti protocol and Annexin-V method) assays were used to assess BDNF functional activity [41].


  1. Expression, subcellular localization and phosphorylation status of annexins 1 and 5 in human pituitary adenomas and a growth hormone-secreting carcinoma. Mulla, A., Christian, H.C., Solito, E., Mendoza, N., Morris, J.F., Buckingham, J.C. Clin. Endocrinol. (Oxf) (2004) [Pubmed]
  2. Cloning and expression of cDNA for human endonexin II, a Ca2+ and phospholipid binding protein. Kaplan, R., Jaye, M., Burgess, W.H., Schlaepfer, D.D., Haigler, H.T. J. Biol. Chem. (1988) [Pubmed]
  3. Differentiation of human colon adenocarcinoma cells alters the expression and intracellular localization of annexins A1, A2, and A5. Guzmán-Aránguez, A., Olmo, N., Turnay, J., Lecona, E., Pérez-Ramos, P., López de Silanes, I., Lizarbe, M.A. J. Cell. Biochem. (2005) [Pubmed]
  4. Correlation between antiphospholipid antibodies that recognize domain I of beta2-glycoprotein I and a reduction in the anticoagulant activity of annexin A5. de Laat, B., Wu, X.X., van Lummel, M., Derksen, R.H., de Groot, P.G., Rand, J.H. Blood (2007) [Pubmed]
  5. Annexin V expression in apoptotic peripheral blood lymphocytes: an electron microscopic evaluation. Cornelissen, M., Philippé, J., De Sitter, S., De Ridder, L. Apoptosis (2002) [Pubmed]
  6. Decreased concentration of annexin V in parkinsonian cerebrospinal fluid: speculation on the underlying cause. Vermes, I., Steur, E.N., Reutelingsperger, C., Haanen, C. Mov. Disord. (1999) [Pubmed]
  7. Microfluidic technology for PET radiochemistry. Gillies, J.M., Prenant, C., Chimon, G.N., Smethurst, G.J., Dekker, B.A., Zweit, J. Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine. (2006) [Pubmed]
  8. Interactions of annexin V with phospholipid monolayers. Mukhopadhyay, S., Cho, W. Biochim. Biophys. Acta (1996) [Pubmed]
  9. Pregnancy loss in the antiphospholipid-antibody syndrome--a possible thrombogenic mechanism. Rand, J.H., Wu, X.X., Andree, H.A., Lockwood, C.J., Guller, S., Scher, J., Harpel, P.C. N. Engl. J. Med. (1997) [Pubmed]
  10. Development and characteristics of a human cell assay for screening agents for melanoma prevention. Elmore, E., Jain, A., Siddiqui, S., Tohidian, N., Meyskens, F.L., Steele, V.E., Redpath, J.L. Melanoma Res. (2007) [Pubmed]
  11. Residue-specific bioincorporation of non-natural, biologically active amino acids into proteins as possible drug carriers: structure and stability of the per-thiaproline mutant of annexin V. Budisa, N., Minks, C., Medrano, F.J., Lutz, J., Huber, R., Moroder, L. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  12. Neutrophils in Barth syndrome (BTHS) avidly bind annexin-V in the absence of apoptosis. Kuijpers, T.W., Maianski, N.A., Tool, A.T., Becker, K., Plecko, B., Valianpour, F., Wanders, R.J., Pereira, R., Van Hove, J., Verhoeven, A.J., Roos, D., Baas, F., Barth, P.G. Blood (2004) [Pubmed]
  13. The selective protein kinase C beta inhibitor enzastaurin induces apoptosis in cutaneous T-cell lymphoma cell lines through the AKT pathway. Querfeld, C., Rizvi, M.A., Kuzel, T.M., Guitart, J., Rademaker, A., Sabharwal, S.S., Krett, N.L., Rosen, S.T. J. Invest. Dermatol. (2006) [Pubmed]
  14. Vinorelbine induces apoptosis and caspase-3 (CPP32) expression in leukemia and lymphoma cells: a comparison with vincristine. Toh, H.C., Sun, L., Koh, C.H., Aw, S.E. Leuk. Lymphoma (1998) [Pubmed]
  15. A novel calcium-dependent proapoptotic effect of annexin 1 on human neutrophils. Solito, E., Kamal, A., Russo-Marie, F., Buckingham, J.C., Marullo, S., Perretti, M. FASEB J. (2003) [Pubmed]
  16. Cell surface-expressed phosphatidylserine and annexin A5 open a novel portal of cell entry. Kenis, H., van Genderen, H., Bennaghmouch, A., Rinia, H.A., Frederik, P., Narula, J., Hofstra, L., Reutelingsperger, C.P. J. Biol. Chem. (2004) [Pubmed]
  17. Nonsecreted insulin-like growth factor binding protein-3 (IGFBP-3) can induce apoptosis in human prostate cancer cells by IGF-independent mechanisms without being concentrated in the nucleus. Bhattacharyya, N., Pechhold, K., Shahjee, H., Zappala, G., Elbi, C., Raaka, B., Wiench, M., Hong, J., Rechler, M.M. J. Biol. Chem. (2006) [Pubmed]
  18. Apoptosis of human macrophages by Flt-4 signaling: implications for atherosclerotic plaque pathology. Schmeisser, A., Christoph, M., Augstein, A., Marquetant, R., Kasper, M., Braun-Dullaeus, R.C., Strasser, R.H. Cardiovasc. Res. (2006) [Pubmed]
  19. Group I mGlu receptor stimulation inhibits activation-induced cell death of human T lymphocytes. Chiocchetti, A., Miglio, G., Mesturini, R., Varsaldi, F., Mocellin, M., Orilieri, E., Dianzani, C., Fantozzi, R., Dianzani, U., Lombardi, G. Br. J. Pharmacol. (2006) [Pubmed]
  20. Annexin V relocates to the platelet cytoskeleton upon activation and binds to a specific isoform of actin. Tzima, E., Trotter, P.J., Orchard, M.A., Walker, J.H. Eur. J. Biochem. (2000) [Pubmed]
  21. Annexin 1-dependent actions of glucocorticoids in the anterior pituitary gland: roles of the N-terminal domain and protein kinase C. John, C., Cover, P., Solito, E., Morris, J., Christian, H., Flower, R., Buckingham, J. Endocrinology (2002) [Pubmed]
  22. Expression of annexins as a function of cellular growth state. Schlaepfer, D.D., Haigler, H.T. J. Cell Biol. (1990) [Pubmed]
  23. Glycosaminoglycan binding properties of annexin IV, V, and VI. Ishitsuka, R., Kojima, K., Utsumi, H., Ogawa, H., Matsumoto, I. J. Biol. Chem. (1998) [Pubmed]
  24. C-reactive protein and annexin A5 bind to distinct sites of negatively charged phospholipids present in oxidized low-density lipoprotein. van Tits, L., de Graaf, J., Toenhake, H., van Heerde, W., Stalenhoef, A. Arterioscler. Thromb. Vasc. Biol. (2005) [Pubmed]
  25. Annexin A5 binding to giant phospholipid vesicles is differentially affected by anti-{beta}2-glycoprotein I and anti-annexin A5 antibodies. Gaspersic, N., Ambrozic, A., Bozic, B., Majhenc, J., Svetina, S., Rozman, B. Rheumatology (Oxford, England) (2007) [Pubmed]
  26. Redistribution and unmasking of Annexin V binding sites in apoptotic Raji cells. di Certo, M.G., Faggioni, A., Barile, G. Cell Biol. Int. (2003) [Pubmed]
  27. Engagement of CD33 surface molecules prevents the generation of dendritic cells from both monocytes and CD34+ myeloid precursors. Ferlazzo, G., Spaggiari, G.M., Semino, C., Melioli, G., Moretta, L. Eur. J. Immunol. (2000) [Pubmed]
  28. Vascular anticoagulant beta: a novel human Ca2+/phospholipid binding protein that inhibits coagulation and phospholipase A2 activity. Its molecular cloning, expression and comparison with VAC-alpha. Hauptmann, R., Maurer-Fogy, I., Krystek, E., Bodo, G., Andree, H., Reutelingsperger, C.P. Eur. J. Biochem. (1989) [Pubmed]
  29. Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annexin V. Cardó-Vila, M., Arap, W., Pasqualini, R. Mol. Cell (2003) [Pubmed]
  30. The role of mitogen-activated protein kinase activation in determining cellular outcomes in polyamine analogue-treated human melanoma cells. Chen, Y., Alm, K., Vujcic, S., Kramer, D.L., Kee, K., Diegelman, P., Porter, C.W. Cancer Res. (2003) [Pubmed]
  31. Zoledronate sensitizes endothelial cells to tumor necrosis factor-induced programmed cell death: evidence for the suppression of sustained activation of focal adhesion kinase and protein kinase B/Akt. Bezzi, M., Hasmim, M., Bieler, G., Dormond, O., Rüegg, C. J. Biol. Chem. (2003) [Pubmed]
  32. Annexin V inhibits the 12-O-tetradecanoylphorbol-13-acetate-induced activation of Ras/extracellular signal-regulated kinase (ERK) signaling pathway upstream of Shc in MCF-7 cells. Sato, H., Ogata, H., De Luca, L.M. Oncogene (2000) [Pubmed]
  33. Altered cardiac annexin mRNA and protein levels in the left ventricle of patients with end-stage heart failure. Song, G., Campos, B., Wagoner, L.E., Dedman, J.R., Walsh, R.A. J. Mol. Cell. Cardiol. (1998) [Pubmed]
  34. Cloning and Characterization of the Annexin II Receptor on Human Marrow Stromal Cells. Lu, G., Maeda, H., Reddy, S.V., Kurihara, N., Leach, R., Anderson, J.L., Roodman, G.D. J. Biol. Chem. (2006) [Pubmed]
  35. Identification and characterization of phospholipase A2 inhibitory proteins in human mononuclear cells. Coméra, C., Rothhut, B., Russo-Marie, F. Eur. J. Biochem. (1990) [Pubmed]
  36. Annexins II, IV, V and VI relocate in response to rises in intracellular calcium in human foreskin fibroblasts. Barwise, J.L., Walker, J.H. J. Cell. Sci. (1996) [Pubmed]
  37. A common haplotype of the annexin A5 (ANXA5) gene promoter is associated with recurrent pregnancy loss. Bogdanova, N., Horst, J., Chlystun, M., Croucher, P.J., Nebel, A., Bohring, A., Todorova, A., Schreiber, S., Gerke, V., Krawczak, M., Markoff, A. Hum. Mol. Genet. (2007) [Pubmed]
  38. The crystal and molecular structure of human annexin V, an anticoagulant protein that binds to calcium and membranes. Huber, R., Römisch, J., Paques, E.P. EMBO J. (1990) [Pubmed]
  39. Gene expression profiling of normal and malignant CD34-derived megakaryocytic cells. Tenedini, E., Fagioli, M.E., Vianelli, N., Tazzari, P.L., Ricci, F., Tagliafico, E., Ricci, P., Gugliotta, L., Martinelli, G., Tura, S., Baccarani, M., Ferrari, S., Catani, L. Blood (2004) [Pubmed]
  40. Chlorpromazine induces apoptosis in activated human lymphoblasts: a mechanism supporting the induction of drug-induced lupus erythematosus? Hieronymus, T., Grötsch, P., Blank, N., Grünke, M., Capraru, D., Geiler, T., Winkler, S., Kalden, J.R., Lorenz, H.M. Arthritis Rheum. (2000) [Pubmed]
  41. Brain-derived neurotrophic factor is increased in atopic dermatitis and modulates eosinophil functions compared with that seen in nonatopic subjects. Raap, U., Goltz, C., Deneka, N., Bruder, M., Renz, H., Kapp, A., Wedi, B. J. Allergy Clin. Immunol. (2005) [Pubmed]
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