Disease relevance of ethanethioic acid

• The antigen identified by MAb WM-25 was isolated from solubilized Mycoplasma strain F38 organisms by MAb WM-25 affinity chromatography and was stained with Schiff's reagent, but not with Coomassie blue, after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis [1].

High impact information on ethanethioic acid

• Another procedure, based on the use of 2-OH-3-naphtoic acid hydrazide coupled with fast blue B, was also developed and proved to be possibly more sensitive than Schiff's reagent in the detection of lipid peroxidation in liver tissue [2].
• This mucin on sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) presented as one, high molecular weight, periodic acid/Schiff's reagent (PAS) stainable band [3].
• Analysis of GIF by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a high-molecular-weight glycoprotein after staining with Coomassie blue or Schiff's reagent [4].
• Selective staining of alpha1-antitrypsin (alpha1-protease inhibitor) with Schiff's reagent after separation from serum by analytical isoelectrofocusing in polyacrylamide gel [5].
• Furthermore, the ANG II-induced increase in COX-2 protein abundance was attenuated by both the peroxisome proliferator-activated receptor alpha (PPARalpha) activator Wy-14,643 [pyrinixic acid; 4-chloro-6-(2,3-xylidino)-2-pyrimidinyl) thioacetic acid] and the PPARgamma activator 15d-PGJ2 (15-deoxy-Delta(12-14)-prostaglandin J2) [6].

Biological context of ethanethioic acid

• Hydrolysis with 5 N HCl and staining with Schiff's reagent of these model films were studied using several parameters [7].
• Sections were cut and stained with anti-VEGF antibodies to evaluate VEGF expression, and with periodic acid and Schiff's reagent to evaluate new bone formation [8].

Anatomical context of ethanethioic acid

• Dentin caries recording with Schiff's reagent, fluorescence, and back-scattered electron image [9].
• Following incubation of the PL (0.5 cm2 sections) with spermatozoa, the PL section was rinsed and stained with Schiff's reagent [10].
• The macronucleus was found to divide unequally, yielding sister pairs having a mean difference in DNA content of 11.6% DNA content was determined by the Feulgen reaction using a fluorescent Schiff's reagent, and measuring fluorescence by cytophotometry [11].
• In addition to trimetaphosphatase activity, the lysosomes are reactive for aryl sulfatase B, thiolacetic acid esterase, and cholinesterase [12].
• Mucin-containing goblet cells were stained by Alcian blue-periodic acid Schiff's reagent and the number of cells per 0.16 mm2 was quantified [13].

Associations of ethanethioic acid with other chemical compounds

• The caries lesions revealed with Schiff's reagent also exhibited a change in the color of the dentin fluorescence regardless of the tetracycline labeling [9].
• The use of borohydrate reduction or treatment with Schiff's reagent to reduce glutaraldehyde-induced background fluorescence is described [14].
• The smear was then bleached with 50% acid ethanol and absolute methanol, and re-stained by the Feulgen method in 0.05% pararosaniline Schiff's reagent (pH 2.3) at 7 degrees C for 10 min [15].
• Histologic examination of the curettage tissue slides stained with hematoxylin and eosin, periodic acid Schiff's reagent, and Gomori's methenamine silver stain procedures showed many broad-based budding cells characteristic of B. dermatitidis [16].
• Histochemical staining with Schiff's reagent and Evans blue revealed that the peroxidation of membrane lipids and loss of plasma membrane integrity in Hg-treated roots occurred in the meristem and the elongation zone [17].

Gene context of ethanethioic acid

• STE was located with immunofluorescence histochemistry, and ARP with Schiff's reagent [18].
• On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the enzyme presented two protein bands corresponding to mol. wts of 65,000 and 55,000, which stained with Schiff's reagent [19].
• Lysosomes were visualized in the anterior pituitary by their non-specific esterase (gold thioacetic acid technique) or acid phosphatase (Gomori technique) activity [20].
• Gallyas technique was modified by a direct application of Schiff's reagent after physical development, resulting in distinctive staining of amyloid deposits in argyrophilic structures [21].

Analytical, diagnostic and therapeutic context of ethanethioic acid

• Expression of mucin antigen was lost on plastic or glass culture, but promoted on collagen gel or matrigel, as demonstrated by staining with periodic acid Schiff's reagent and anti-mucin monoclonal antibody stainings [22].
• After electrofocusing serum from human and various animals in the pH range 4.0-6.0, only the isoproteins of antitrypsin stain with Schiff's reagent [5].
• Frozen sections of the thyroids and livers from iodine-treated rats were compared to tap-water controls after staining with Mercury Orange for GSH and Schiff's reagent for evidence of lipid peroxidation [23].
• The suspensory apparatus of the canine lens was studied in gross specimens stained with periodic acid and Schiff's reagent, and by transmission and scanning electron microscopy [24].
• The specificity of the staining reaction was increased by incubation of the sections in NH4Cl and Schiff's reagent prior to Ag-NOR silver staining [25].

References