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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
MeSH Review

Cytophotometry

 
 
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Disease relevance of Cytophotometry

 

High impact information on Cytophotometry

 

Chemical compound and disease context of Cytophotometry

 

Biological context of Cytophotometry

  • Continuous incubation with anguidine for longer than 48 hr produced a moderate increase in the percentage of S-phase cells and a slight decrease in the proportion of cells in G1/0, by pulse cytophotometry [13].
  • Physical proximity between fluorochrome-labeled CR3 and Fc gamma RIIIB on individual cells was measured by resonance energy transfer (RET) imaging, quantitative RET microfluorometry, and single-cell imaging spectrophotometry [14].
  • The present study reports results obtained with DNA ploidy measurement by Feulgen cytophotometry and in situ hybridization (ISH) on the same sections of 36 cervical lesions containing human papillomavirus (HPV) DNA [15].
  • After stoichiometric azure B and Feulgen staining of brain sections, scanning-integrating cytophotometry was used to quantify azure B-ribonucleic acid (RNA) content, Feulgen-DNA levels, and changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) of neurons on an individual basis [16].
  • Mechanisms underlying the Ca2+ increase during tetanic synaptic inputs in layer II/III of visual cortical slices of young rats were investigated with microfluorometry using a Ca2+ indicator, rhod-2, and simultaneous recordings of field potentials evoked by white matter stimulation [17].
 

Anatomical context of Cytophotometry

 

Associations of Cytophotometry with chemical compounds

 

Gene context of Cytophotometry

  • The mGluR5 receptor function was assessed in CG-4 OPCs with fura-2 microfluorometry [28].
  • PACAP release from islets was bioassayed using the response of cytosolic Ca2+ concentration ([Ca2+]i) in single beta-cells, monitored by dual-wavelength fura-2 microfluorometry [29].
  • As an initial investigation, we characterized the ability of specific mGluR subtypes to modulate iCa(2+) by using Fura-2 microfluorometry in primary hippocampal neurons [30].
  • In this study, we compared changes of [Ca2+]i in single beta-cells isolated from ob/ob mice with those from lean mice using dual-wavelength microfluorometry and the indicator fura-2 [31].
  • Microfluorometry of single cells was performed in six cases to estimate the range of CEA content [32].
 

Analytical, diagnostic and therapeutic context of Cytophotometry

  • Autoradiography and cytophotometry of cells of carbohydrate-starved root tips revealed that extrachromosomal DNA was produced by a small fraction of cells accumulated in the late S phase after they had replicated about 80% of their DNA [33].
  • Luminal Cl- concentration was also measured by microfluorometry following microinjection of the dextran conjugates of 6-methoxy-N-(4-aminoalkyl)quinolinium bromide (ABQ-dextran) and Cl-NERF (Cl-NERF-dextran) [34].
  • 1. Ca2+ microfluorometry (100 microM K5 fura-2) and the voltage-clamp technique were combined to study the effect of carbachol (CCh, 50 microM) in inducing currents (ICCh) through non-selective cation channels (NSCCCh) and increments in global cytosolic Ca2+ concentration (Delta[Ca2+]c) [35].
  • Effects of steroids on the accumulation of glycoprotein gonadotropin (GTH) in pituitaries of juvenile trout were investigated by means of scanning cytophotometry applied to immunocytochemical preparations, and with the use of a radioimmunoassay [36].
  • Changes in intracellular Ca2+ evoked by electrical stimulation of the white matter were observed by means of microfluorometry with a Ca2+ indicator, rhod-2, in slice preparations of the visual cortex obtained from young rats [37].

References

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  3. Quantitative cytochemical differences between young and old patients with lung cancer. Huang, M.S., Kato, H., Konaka, C., Nishimiya, K., Yoneyama, K., Kawate, N., Katsunuma, H., Hayata, Y. Chest (1985) [Pubmed]
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  15. DNA content measurement and in situ hybridization in condylomatous cervical lesions. Clavel, C., Zerat, L., Binninger, I., Boutterin, M.C., Polette, M., Monsonego, J., Birembaut, P. Diagn. Mol. Pathol. (1992) [Pubmed]
  16. Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites. Martin, L.J., Morse, J.D., Pritchard, G.A., Kim, Y.H., Anthony, A. J. Neurol. Sci. (1988) [Pubmed]
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  22. Development of mechanisms regulating intracellular Ca2+ concentration in cardiac muscle cells of early chick embryos. Komazaki, S., Hiruma, T. Dev. Biol. (1997) [Pubmed]
  23. Secretagogue-induced mobilization of an intracellular Mg2+ pool in rat sublingual mucous acini. Zhang, G.H., Melvin, J.E. J. Biol. Chem. (1992) [Pubmed]
  24. High glucose level inhibits capacitative Ca2+ influx in cultured rat mesangial cells by a protein kinase C-dependent mechanism. Menè, P., Pugliese, G., Pricci, F., Di Mario, U., Cinotti, G.A., Pugliese, F. Diabetologia (1997) [Pubmed]
  25. Cytophotometric and flow cytometric DNA content of isolated glands in gastric neoplasia. Kitayama, Y., Nakamura, S., Sugimura, H., Kino, I. Gut (1995) [Pubmed]
  26. Dexamethasone aggravates ischemia-induced neuronal damage by facilitating the onset of anoxic depolarization and the increase in the intracellular Ca2+ concentration in gerbil hippocampus. Adachi, N., Chen, J., Liu, K., Tsubota, S., Arai, T. J. Cereb. Blood Flow Metab. (1998) [Pubmed]
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  28. Functional metabotropic glutamate receptors are expressed in oligodendrocyte progenitor cells. Luyt, K., Varadi, A., Molnar, E. J. Neurochem. (2003) [Pubmed]
  29. Pituitary adenylate cyclase-activating polypeptide (PACAP) is an islet substance serving as an intra-islet amplifier of glucose-induced insulin secretion in rats. Yada, T., Sakurada, M., Ishihara, H., Nakata, M., Shioda, S., Yaekura, K., Hamakawa, N., Yanagida, K., Kikuchi, M., Oka, Y. J. Physiol. (Lond.) (1997) [Pubmed]
  30. Metabotropic glutamate receptor subtypes independently modulate neuronal intracellular calcium. Maiese, K., Ahmad, I., TenBroeke, M., Gallant, J. J. Neurosci. Res. (1999) [Pubmed]
  31. Pancreatic beta-cells from obese-hyperglycemic mice are characterized by excessive firing of cytoplasmic Ca2+ transients. Ahmed, M., Grapengiesser, E. Endocrine (2001) [Pubmed]
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