Disease relevance of Fmrfamide-like octapeptide

• To elucidate the mechanism of the cellular antiopioid activity of neuropeptide FF (NPFF), we have transfected the SH-SY5Y neuroblastoma cell line, which expresses mu-and delta-opioid receptors, with the human NPFF2receptor [1].
• Neuropeptide FF (NPFF), an FMRFamide-like peptide with antiopioid properties, inhibits morphine-induced analgesia but also produces hyperalgesia [2].
• In this study neuropeptide FF and another RF-amide peptide, the prolactin-releasing peptide mRNA levels were studied by quantitative in situ hybridization after chronic salt loading, a laboratory model of chronic dehydration [3].
• Opposing interplay between Neuropeptide FF and nitric oxide in antinociception and hypothermia [4].
• Cholera and pertussis toxins inhibit differently hypothermic and anti-opioid effects of neuropeptide FF [5].

Psychiatry related information on Fmrfamide-like octapeptide

• Changes in CNS neuropeptide FF-like material, pain sensitivity, and opiate dependence following chronic morphine treatment [6].

High impact information on Fmrfamide-like octapeptide

• Anti-opioid effects of neuropeptide FF were found to require a functional PrRP-GPR10 system [7].
• We found that neuropeptide FF (Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe amide) and FMRFamide (Phe-Met-Arg-Phe amide) generated no current on their own but potentiated H+-gated currents from cultured sensory neurons and heterologously expressed ASIC and DRASIC channels [8].
• Neuropeptide FF and FMRFamide potentiate acid-evoked currents from sensory neurons and proton-gated DEG/ENaC channels [8].
• Neuropeptide FF (NPFF) has been proposed to play a role in pain modulation, opioid tolerance, and several other physiological processes [9].
• Identification and characterization of two G protein-coupled receptors for neuropeptide FF [10].

Biological context of Fmrfamide-like octapeptide

• Analysis of human neuropeptide FF gene expression [11].
• The homologous regulation of hNPFF2 receptor correlates with our previous results in vivo showing that during inflammation, the up-regulation of neuropeptide FF mRNA precedes that of NPFF2 receptor [12].
• As an initial step to study the function of the gene encoding the human neuropeptide FF (NPFF), we cloned a 4.7-kb sequence from the promoter region [11].
• Among these antiopioids, neuropeptide FF (NPFF) is particularly interesting since both NPFF immunoreactive-like terminals and NPFF binding sites are located in the vicinity of the dopaminergic cell bodies within the ventral tegmental area (VTA) suggesting an interaction at this level [13].
• The early appearance and the distribution of the F8Famide-like immunoreactivity suggest that these peptides may participate, in addition to nociceptive mechanisms, in the regulation of blood pressure, feeding behaviour, and endocrine functions [14].

Anatomical context of Fmrfamide-like octapeptide

• Neuropeptide FF (NPFF) and neuropeptide AF (NPAF) are two mammalian amidated neuropeptides which are highly concentrated in the posterior pituitary, spinal cord, hypothalamus and medulla [15].
• The central nervous system octapeptide, neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance [10].
• Using a radioligand binding assay, we examined ionic modulation and G protein coupling of neuropeptide FF (NPFF) receptors in membranes of rat brain and spinal cord [16].
• Upon neuropeptide FF treatment, the actin cytoskeleton was reorganized in the cells [12].
• Activation of neuropeptide FF neurons in the brainstem nucleus tractus solitarius following cardiovascular challenge and opiate withdrawal [17].

Associations of Fmrfamide-like octapeptide with other chemical compounds

• In Wistar rats neuropeptide FF was present in part of the magnocellular neurones of the paraventricular and supraoptic nuclei in which it was coexpressed with vasopressin [18].
• The neuropeptide FF analogue, 1DME, enhances in vivo met-enkephalin release from the rat spinal cord [19].
• Distribution of neuropeptide FF in porcine spinal cord in comparison with other neuropeptides and serotonin [20].
• Taken together, these results suggest a relationship between neuropeptide FF and NMDA receptors in the spinal cord [21].
• In addition, the neuropeptide FF-induced heart rate decrease was abolished by pretreatment with atropine (30 micrograms/kg), a muscarinic receptor antagonist [22].

Gene context of Fmrfamide-like octapeptide

• Regulation of endogenous human NPFF2 receptor by neuropeptide FF in SK-N-MC neuroblastoma cell line [12].
• These properties of NPY ligands on Neuropeptide FF receptors must be considered when evaluating pharmacological activities of these drugs [23].
• ASIC3-mediated sustained depolarisation, and its regulation by neuropeptides, could thus be important in regulating polymodal neuron excitability particularly under inflammatory conditions where the expression levels of both NPFF precursor and ASIC3 are increased [24].
• GPR103 shared identities with the neuropeptide FF 2, neuropeptide Y2 and galanin GalR1 receptors (34-38% in the TM regions) [25].
• Two G protein-coupled receptors (NPFF1 and NPFF2 receptors) have been identified for neuropeptide FF [12].

Analytical, diagnostic and therapeutic context of Fmrfamide-like octapeptide

• Intrathecal infusion of the neuropeptide FF analogue, [D-Tyr1, (NMe)Phe3]neuropeptide FF (1DMe; 0.1 microm-0.1 mm) in anaesthetized rats produced a concentration-dependent decrease in the spinal outflow of dynorphin A (1-8)-like material, which persisted for at least 90 min after treatment with 10 microm-0.1 mm of the compound [26].
• Good correlation between our results and receptor autoradiography support the idea that the terminal areas which our results show are target areas of the neuropeptide FF-containing system [27].
• A novel neuropeptide of the RFamide peptide family was isolated in pure form from a frog (Rana esculenta) brain extract by using reversed-phase high performance liquid chromatography in combination with a radioimmunoassay for mammalian neuropeptide FF (NPFF) [28].
• Immunocytochemical localization of neuropeptide FF (FMRF amide-like peptide) in the hypothalamo-neurohypophyseal system of Wistar and Brattleboro rats by light and electron microscopy [18].
• The effect of neuropeptide FF in the periaqueductal gray on pain behaviour was studied in rats with a chronic neuropathy induced by unilateral ligation of two spinal nerves [29].

References