Disease relevance of Scintillation Counting

• Quantitative changes in the relative expression levels of [alpha 32P]GTP-binding proteins in normal proliferating and simian virus 40 (SV40) transformed human keratinocytes (K 14) were determined by scintillation counting of the radioactive spots excised from the nitrocellulose blots [1].
• A correlation between 19F MRS and scintillation counting measures of tumor-bound, tritium-labeled hypoxia marker was observed [2].
• The total ouabain dosage required for ventricular tachycardia was recorded, and the ouabain concentrations in the arterial plasma and in the myocardium were determined by liquid scintillation counting [3].
• Specific localization in vivo of marked, 131I-labelled human chorionic gonadotrophin (HCG) was shown by scintillation counting of different tissues to occur in rat ovarian tumor, but not in human Krukenberg tumor heterografts growing subcutaneously in female nude mice [4].
• Blood platelets from patients with myoclonus epilepsy and those from healthy sex and age matched controls were incubated for 5 min with 0.1 microM 3H-5-HT in Krebs-Henseleit buffer at 37 degrees C. The radioactivity taken up by platelets was assayed by liquid scintillation counting [5].

High impact information on Scintillation Counting

• Three mutant clones were established, which have remained hypoxanthine phosphoribosyltransferase (HPRT) deficient for a period of 7 months, as shown by indirect measurements with the use of autoradiography and scintillation counting of cells exposed to [3H]hypoxanthine [6].
• A steady-state tracer technique was employed using oxygen-15, carbon-11, and nitrogen-13 isotopes and external scintillation counting of the 511-KeV annihilation radiation common to all three radionuclides [7].
• After ingestion of galactose (10 g per m2) labeled with 14C or 13C, breath was collected from subjects at intervals for 4 hr followed by measurement of 14CO2 by liquid scintillation counting or of 13CO2 by mass spectrometry [8].
• Quantitation, performed by scintillation counting of 32P-dCTP incorporated into specific globin cDNA bands, showed ratios of alpha/beta-globin mRNA greater than 10-fold and greater than fivefold increased in patients with beta 0- and beta (+)-thal, respectively, as well as a relative increase in gamma-globin mRNA levels [9].
• The resulting tritiated product, guaiacol, is readily extracted into toluene and quantified by scintillation counting [10].

Chemical compound and disease context of Scintillation Counting

• Tritiated 5-FU (200 microCi) was injected into the inferior mesenteric artery of 8 perfused specimens of human large bowel containing adenocarcinoma and the concentration of 5-FU in local tissues and venous effluent was measured by scintillation counting [11].

Biological context of Scintillation Counting

• Flat-bed scintillation counting was used to detect and separate the emissions resulting from each bound probe, and gene dosage was calculated from the ratio of INT-2 to the beta-actin probe compared with the ratio derived from constitutional DNA [12].
• After inhalation of 5 microns technetium 99m labelled particles under controlled conditions, whole lung clearance was monitored by scintillation counters half hourly for six hours with a final count at 24 hours, from which tracheobronchial deposition and clearance could be calculated [13].
• ATPgammaS-induced proliferation was demonstrated by three separate methods, detection by scintillation counting of [3H]thymidine incorporation, immunocytochemical detection of 5-bromo-2-deoxyuridine incorporation and direct counting of cell numbers [14].
• Tumor cell adhesion to the endothelium was quantified for 6 hours using cells labeled with sulfur 35 and a scintillation counter [15].
• All treated cells preferentially incorporated [3H] thymidine as a result of DNA repair monitored by liquid scintillation counting of the extracted DNA [16].

Anatomical context of Scintillation Counting

• At the time of maximal deoxyribonucleic acid (DNA) synthesis in these cells, significant amounts of 3H bleomycin are demonstrable in the lung by scintillation counting; and in autoradiographs, the drug appears to concentrate in epithelial cells [17].
• Crude cell wall and cytoplasmic antigens were derived from Rothia dentocariosa (RD), were applied to lymphocyte microcultures, and subjected to radioactive thymidine; the resulting lymphocyte blastogenesis (LB) was surveyed with a scintillation counter [18].
• Lymphoid cells similarly injected were traced by several techniques: 1) scintillation counting of tissues for 51Cr-labeled cells; 2) autoradiography for [3H]uridine-labeled cells; 3) fluorescent microscopy for fluorescein-containing cells in frozen tissues; and 4) graft-versus-host reactivity in draining lymph nodes [19].
• Bretylium concentrations in plasma and amounts excreted in urine and feces were determined by scintillation counting [20].
• METHODS: Transport experiments were conducted using Caco-2 cell monolayers and either reversed-phase HPLC-UV or liquid scintillation counting methods for quantification. pKa, LogD, and LogP were determined using the Sirius GlpKa meter [21].

Associations of Scintillation Counting with chemical compounds

• Cells which were able to actively cross the entire BM were trapped on filters and counted by both brightfield microscopy and by beta scintillation counting of cells whose DNA was labeled with tritiated thymidine [22].
• The radioactive phenylthiohydantoin derivatives produced in the degradation are identified at the 10 to 100 pmol level by two-dimensional thin layer chromatographic procedures with autoradiography and are quantitated by scintillation counting [23].
• Protein bands were visualized by staining with Coomassie Brilliant Blue, excised, and counted in a liquid scintillation counter [24].
• The cells were pulse labeled with [3H]serine whereafter the distribution of the labels among PS and PE molecular species was determined by reverse phase high performance liquid chromatography and liquid scintillation counting [25].
• The retention and loss of [3H]5-hydroxytryptamine (5-HT) from rabbit platelets during fixation was studied using liquid scintillation counting and quantitative electron-microscopic autoradiography [26].

Gene context of Scintillation Counting

• The proliferative response of CBMC stimulated with IL-2, betalactoglobulin (BLG) and house dust mite allergen (Der p 1) was assessed by liquid scintillation counting and the stimulation index (SI) was calculated [27].
• Finally, drug uptake studies with [3H]estramustine, scintillation counting and fluorography demonstrated that the principal target of the drug was MAP-1A [28].
• After incubation for 24 h with various concentrations of adrenomedullin in the presence of 5% FCS, trichloroacetic acid-insoluble tritiated thymidine was measured in a liquid scintillation counter [29].
• A radiochromatographic method has been set up in order to determine fatty acid amide hydrolase (FAAH) activity, based on reversed-phase high-performance liquid chromatography and on-line scintillation counting [30].
• Northern blots and scintillation counting showed that tissue inhibitor of metalloproteinase 1 (TIMP-1) mRNA was expressed by low passage, primary epithelial cultures (n = 5) of low-grade human prostatic carcinoma [31].

Analytical, diagnostic and therapeutic context of Scintillation Counting

• After incubation for time periods up to 120 min, cells and incubation media were analyzed for labelled and endogenous glutamate and its metabolic products by HPLC coupled with fluorescence detection and liquid scintillation counting [32].
• Prostaglandin and thromboxane products (cyclo-oxygenase products) were identified by thin-layer chromatography and quantified by scintillation counting [33].
• The GAGs were precipitated from the media and cell layer with cetylpyridinium chloride and ethanol, separated in 0.1 M Ba acetate by cellulose acetate electrophoresis, visualized by Alcian blue staining and fluorography, and quantitated by scanning densitometry and liquid scintillation counting [34].
• [(1967) Arch. Biochem. Biophys. 121, 29], which were obtained by countercurrent distribution of tritium-labeled products and their identification by scintillation counting [35].
• Assays of thiopental by gas chromatography and 14C scintillation counting gave similar results [36].

References