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Gene Review

CRIP1  -  cysteine-rich protein 1 (intestinal)

Homo sapiens

Synonyms: CRHP, CRIP, CRP-1, CRP1, Cysteine-rich heart protein, ...
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Disease relevance of CRIP1


High impact information on CRIP1


Biological context of CRIP1


Anatomical context of CRIP1

  • We have cloned a human CRIP cDNA from human small intestine poly(A)+ RNA by RT-PCR [11].
  • Incubation of THP-1 cells with 65Zn and chromatography of the cytosol show that a significant amount of the radioactivity is associated with CRIP as was shown previously for rat intestine [11].
  • Recently, we have shown that adhesion of primary human fibroblasts to immobilized Cyr61 is mediated through integrin alpha(6)beta(1) and cell surface heparan sulfate proteoglycans (HSPGs) (Chen, N., Chen, C.-C., and Lau, L.F. (2000) J [15].
  • The tissue concentration of CRIP is of the order of 15-20 micrograms/g of mucosal tissue, suggesting that the protein is more abundant than zinc-finger-containing transcription factors [16].
  • beta-Microseminoprotein (MSP) is a small cysteine-rich protein (molecular mass about 10 kDa) first isolated from human seminal plasma and later identified in several other organisms [17].

Associations of CRIP1 with chemical compounds


Physical interactions of CRIP1

  • Mutation of the syndecan-binding site (PPRR --> PPTM) within this domain abolishes binding of the recombinant disintegrin and cysteine-rich domains of ADAM13 [18].

Other interactions of CRIP1

  • Members of the IGFBP family share conserved cysteine-rich amino- and carboxyl-terminal regions [22].
  • The angiogenic factors Cyr61 and connective tissue growth factor induce adhesive signaling in primary human skin fibroblasts [15].
  • The duplication of nucleotides in exon 1 is located in the conserved cysteine-rich N-terminal region that corresponds to the whey acidic protein motif, affecting the KAL1 protein either by interrupting the normal transcription or stopping the translation at the stop codon [23].
  • Structure and sequence comparisons have revealed an evolutionary relationship between the N-terminal sub-domain of the CR module and the fibronectin type 1 domain, suggesting that these domains share a common ancestry [24].
  • ADAM 9 is a member of the cellular metalloprotease/disintegrin/cysteine-rich (MDC) gene family, related to soluble snake venom metalloproteases (SVMP) [25].

Analytical, diagnostic and therapeutic context of CRIP1

  • Sequence analysis has shown that this human cysteine-rich heart protein (hCRHP) is a protein of 77 amino acids and possesses a LIM motif which is considered to be able to bind zinc [2].
  • Through sequencing, we found that the human intestinal CRIP protein (hCRIP) differed from the previously cloned rat CRIP by two amino acids (residues 8 and 58). hCRIP was expressed with the pET vector/bacterial system and isolated by gel filtration and ion-exchange chromatography [11].
  • Using surface plasmon resonance, the PIII SVMP jararhagin and a recombinant cysteine-rich domain from a PIII SVMP were demonstrated to bind to collagen XIV, collagen XII, and matrilins 1, 3, and 4 [26].


  1. Mapping of the human cysteine-rich intestinal protein gene CRIP1 to the human chromosomal segment 7q11.23. Garcia-Barcelo, M., Tsui, S.K., Chim, S.S., Fung, K.P., Lee, C.Y., Waye, M.M. Genomics (1998) [Pubmed]
  2. Isolation and characterization of a cDNA that codes for a LIM-containing protein which is developmentally regulated in heart. Tsui, S.K., Yam, N.Y., Lee, C.Y., Waye, M.M. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
  3. Fibronectin self-association is mediated by complementary sites within the amino-terminal one-third of the molecule. Aguirre, K.M., McCormick, R.J., Schwarzbauer, J.E. J. Biol. Chem. (1994) [Pubmed]
  4. Trigramin, an RGD-containing peptide from snake venom, inhibits cell-substratum adhesion of human melanoma cells. Knudsen, K.A., Tuszynski, G.P., Huang, T.F., Niewiarowski, S. Exp. Cell Res. (1988) [Pubmed]
  5. Recombinant retroviruses containing novel reporter genes. Schreiber, J.H., Schisa, J.A., Wilson, J.M. BioTechniques (1993) [Pubmed]
  6. Amino acid sequence of mouse tenascin and differential expression of two tenascin isoforms during embryogenesis. Weller, A., Beck, S., Ekblom, P. J. Cell Biol. (1991) [Pubmed]
  7. The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. Tu, Y., Li, F., Goicoechea, S., Wu, C. Mol. Cell. Biol. (1999) [Pubmed]
  8. Extracellular human immunodeficiency virus type 1 Tat protein promotes aggregation and adhesion of cerebellar neurons. Orsini, M.J., Debouck, C.M., Webb, C.L., Lysko, P.G. J. Neurosci. (1996) [Pubmed]
  9. Argos mutants define an affinity threshold for spitz inhibition in vivo. Alvarado, D., Evans, T.A., Sharma, R., Lemmon, M.A., Duffy, J.B. J. Biol. Chem. (2006) [Pubmed]
  10. Increased B cell survival and preferential activation of the memory compartment by a malaria polyclonal B cell activator. Donati, D., Mok, B., Chêne, A., Xu, H., Thangarajh, M., Glas, R., Chen, Q., Wahlgren, M., Bejarano, M.T. J. Immunol. (2006) [Pubmed]
  11. Human cysteine-rich intestinal protein: cDNA cloning and expression of recombinant protein and identification in human peripheral blood mononuclear cells. Khoo, C., Blanchard, R.K., Sullivan, V.K., Cousins, R.J. Protein Expr. Purif. (1997) [Pubmed]
  12. Characterization of the murine macrophage mannose receptor: demonstration that the downregulation of receptor expression mediated by interferon-gamma occurs at the level of transcription. Harris, N., Super, M., Rits, M., Chang, G., Ezekowitz, R.A. Blood (1992) [Pubmed]
  13. Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to "integrin". Fitzgerald, L.A., Steiner, B., Rall, S.C., Lo, S.S., Phillips, D.R. J. Biol. Chem. (1987) [Pubmed]
  14. A novel activating anti-beta1 integrin monoclonal antibody binds to the cysteine-rich repeats in the beta1 chain. Faull, R.J., Wang, J., Leavesley, D.I., Puzon, W., Russ, G.R., Vestweber, D., Takada, Y. J. Biol. Chem. (1996) [Pubmed]
  15. The angiogenic factors Cyr61 and connective tissue growth factor induce adhesive signaling in primary human skin fibroblasts. Chen, C.C., Chen, N., Lau, L.F. J. Biol. Chem. (2001) [Pubmed]
  16. Purification and properties of rat cysteine-rich intestinal protein. Khoo, C., Cousins, R.J. Biochem. J. (1994) [Pubmed]
  17. Solution Structures of Human and Porcine beta-Microseminoprotein. Ghasriani, H., Teilum, K., Johnsson, Y., Fernlund, P., Drakenberg, T. J. Mol. Biol. (2006) [Pubmed]
  18. ADAM13 disintegrin and cysteine-rich domains bind to the second heparin-binding domain of fibronectin. Gaultier, A., Cousin, H., Darribère, T., Alfandari, D. J. Biol. Chem. (2002) [Pubmed]
  19. Identification of FLRT1, FLRT2, and FLRT3: a novel family of transmembrane leucine-rich repeat proteins. Lacy, S.E., Bönnemann, C.G., Buzney, E.A., Kunkel, L.M. Genomics (1999) [Pubmed]
  20. Insulin-like growth factor binding protein-1: recent findings and new directions. Lee, P.D., Giudice, L.C., Conover, C.A., Powell, D.R. Proc. Soc. Exp. Biol. Med. (1997) [Pubmed]
  21. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Guo, M., Gong, S., Maric, S., Misulovin, Z., Pack, M., Mahnke, K., Nussenzweig, M.C., Steinman, R.M. Hum. Immunol. (2000) [Pubmed]
  22. Ligand-binding characteristics of recombinant amino- and carboxyl-terminal fragments of human insulin-like growth factor-binding protein-3. Galanis, M., Firth, S.M., Bond, J., Nathanielsz, A., Kortt, A.A., Hudson, P.J., Baxter, R.C. J. Endocrinol. (2001) [Pubmed]
  23. Identification of three novel mutations in the KAL1 gene in patients with Kallmann syndrome. Söderlund, D., Canto, P., Méndez, J.P. J. Clin. Endocrinol. Metab. (2002) [Pubmed]
  24. Solution structure and dynamics of a prototypical chordin-like cysteine-rich repeat (von Willebrand Factor type C module) from collagen IIA. O'Leary, J.M., Hamilton, J.M., Deane, C.M., Valeyev, N.V., Sandell, L.J., Downing, A.K. J. Biol. Chem. (2004) [Pubmed]
  25. Cloning and expression in Pichia pastoris of metalloprotease domain of ADAM 9 catalytically active against fibronectin. Schwettmann, L., Tschesche, H. Protein Expr. Purif. (2001) [Pubmed]
  26. The cysteine-rich domain of snake venom metalloproteinases is a ligand for von willebrand factor a domains: role in substrate targeting. Serrano, S.M., Kim, J., Wang, D., Dragulev, B., Shannon, J.D., Mann, H.H., Veit, G., Wagener, R., Koch, M., Fox, J.W. J. Biol. Chem. (2006) [Pubmed]
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