The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

P2rx3  -  purinergic receptor P2X, ligand-gated ion...

Mus musculus

Synonyms: 4930513E20Rik, ATP receptor, P2X purinoceptor 3, P2X3, Purinergic receptor
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of P2rx3


High impact information on P2rx3

  • Immunohistochemical studies localize P2X3 to nerve fibres innervating the urinary bladder of wild-type mice, and show that loss of P2X3 does not alter sensory neuron innervation density [2].
  • P2X3 deficiency also causes a reduction in the sustained ATP-induced currents in nodose ganglion neurons [2].
  • The ATP receptor P2X3 is selectively expressed by nociceptors and is one of seven ATP-gated, cation-selective ion channels [1].
  • Thus, P2X3 is critical for peripheral pain responses and afferent pathways controlling urinary bladder volume reflexes [2].
  • P2X3-null mice have reduced pain-related behaviour in response to injection of ATP and formalin [2].

Biological context of P2rx3

  • Here we demonstrate that ablation of the P2X3 gene results in the loss of rapidly desensitizing ATP-gated cation currents in dorsal root ganglion neurons, and that the responses of nodose ganglion neurons to ATP show altered kinetics and pharmacology resulting from the loss of expression of P2X(2/3) heteromultimers [1].
  • Peristalsis is impaired in the small intestine of mice lacking the P2X3 subunit [4].
  • These results suggest that cutaneous barrier homeostasis can be regulated by cation flux through a P2X3-like ATP receptor [5].
  • Taken together, these data extend our findings for P2X3-/- mice, and reveal an important contribution of heteromeric P2X2/3 receptors to nociceptive responses and mechanosensory transduction within the urinary bladder [6].
  • RT-PCR analysis of mRNA from intestinal tissues verified P2X3 gene deletion [4].

Anatomical context of P2rx3

  • GFP was also expressed in a subpopulation of the primary sensory neurons immunoreactive for the vanilloid receptor TRPV1 and the ATP-gated ion channel P2X3 [7].
  • As previously reported, recording from the spinal cord dorsal horn of anesthetized P2X3-/- mice revealed a blunted response of wide dynamic range neurons to hind paw heating [8].
  • Afferent cell bodies in thoracolumbar and lumbosacral dorsal root ganglia (DRG) were retrogradely labelled from the colon and analysed for P2X3- and TRPV1-like immunoreactivity (LI) [9].
  • These data indicate that P2X receptors on sensory and sympathetic ganglion neurones involve almost exclusively P2X2 and P2X3 subunits [6].
  • It is concluded that P2X3 subunit-containing receptors participate in neural pathways underlying peristalsis in the mouse intestine in vitro [4].

Associations of P2rx3 with chemical compounds

  • ATP, but not alpha,beta-methylene ATP, caused depolarization of AH neurons from P2X3-/- mice [4].
  • Topical application of suramin (nonspecific ATP receptor antagonist), pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (PPADS) (P2X receptor antagonist), and 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate (TNP-ATP) (P2X1, P2X3, P2X2/3 antagonist) after barrier disruption accelerated the barrier repair [5].
  • Peristalsis was inhibited in ileal segments from P2X3-/- mice but longitudinal muscle contractions caused by nicotine and bethanechol were similar in segments from P2X3+/+ and P2X3-/- mice [4].
  • This response was completely blocked by intrathecal pretreatment with the non-selective P2 receptor antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonate (PPADS) and the selective P2X1, P2X3 and P2X2-3 receptor antagonist, 2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-triphosphate (TNP-ATP) [10].
  • Genetic elimination of ionotropic purinergic receptors (P2X2 and P2X3) eliminates taste responses in the taste nerves, although the nerves remain responsive to touch, temperature, and menthol [11].

Other interactions of P2rx3

  • In addition, OSMR-positive neurons decreased, resulting in the reduced number of VR1/P2X3/OSMR-triple positive neurons [12].
  • The RAG locus-associated gene T160 is located 1.8 kb upstream of the transcription start site of mouse P2X3 gene [3].
  • Using RNase protection and primer extension assays, multiple transcription initiation sites have been mapped in the mouse P2X3 promoter to a region 162-168 bp upstream of the translation initiation codon [3].

Analytical, diagnostic and therapeutic context of P2rx3


  1. Warm-coding deficits and aberrant inflammatory pain in mice lacking P2X3 receptors. Souslova, V., Cesare, P., Ding, Y., Akopian, A.N., Stanfa, L., Suzuki, R., Carpenter, K., Dickenson, A., Boyce, S., Hill, R., Nebenuis-Oosthuizen, D., Smith, A.J., Kidd, E.J., Wood, J.N. Nature (2000) [Pubmed]
  2. Urinary bladder hyporeflexia and reduced pain-related behaviour in P2X3-deficient mice. Cockayne, D.A., Hamilton, S.G., Zhu, Q.M., Dunn, P.M., Zhong, Y., Novakovic, S., Malmberg, A.B., Cain, G., Berson, A., Kassotakis, L., Hedley, L., Lachnit, W.G., Burnstock, G., McMahon, S.B., Ford, A.P. Nature (2000) [Pubmed]
  3. Structure and chromosomal mapping of the mouse P2X3 gene. Souslova, V., Ravenall, S., Fox, M., Wells, D., Wood, J.N., Akopian, A.N. Gene (1997) [Pubmed]
  4. Peristalsis is impaired in the small intestine of mice lacking the P2X3 subunit. Bian, X., Ren, J., DeVries, M., Schnegelsberg, B., Cockayne, D.A., Ford, A.P., Galligan, J.J. J. Physiol. (Lond.) (2003) [Pubmed]
  5. P2X purinergic receptor antagonist accelerates skin barrier repair and prevents epidermal hyperplasia induced by skin barrier disruption. Denda, M., Inoue, K., Fuziwara, S., Denda, S. J. Invest. Dermatol. (2002) [Pubmed]
  6. P2X2 knockout mice and P2X2/P2X3 double knockout mice reveal a role for the P2X2 receptor subunit in mediating multiple sensory effects of ATP. Cockayne, D.A., Dunn, P.M., Zhong, Y., Rong, W., Hamilton, S.G., Knight, G.E., Ruan, H.Z., Ma, B., Yip, P., Nunn, P., McMahon, S.B., Burnstock, G., Ford, A.P. J. Physiol. (Lond.) (2005) [Pubmed]
  7. Characterization of sensory neuron subpopulations selectively expressing green fluorescent protein in phosphodiesterase 1C BAC transgenic mice. Torsney, C., Anderson, R.L., Ryce-Paul, K.A., MacDermott, A.B. Molecular pain [electronic resource] (2006) [Pubmed]
  8. Enhanced thermal avoidance in mice lacking the ATP receptor P2X3. Shimizu, I., Iida, T., Guan, Y., Zhao, C., Raja, S.N., Jarvis, M.F., Cockayne, D.A., Caterina, M.J. Pain (2005) [Pubmed]
  9. Differential chemosensory function and receptor expression of splanchnic and pelvic colonic afferents in mice. Brierley, S.M., Carter, R., Jones, W., Xu, L., Robinson, D.R., Hicks, G.A., Gebhart, G.F., Blackshaw, L.A. J. Physiol. (Lond.) (2005) [Pubmed]
  10. In vivo pathway of thermal hyperalgesia by intrathecal administration of alpha,beta-methylene ATP in mouse spinal cord: involvement of the glutamate-NMDA receptor system. Tsuda, M., Ueno, S., Inoue, K. Br. J. Pharmacol. (1999) [Pubmed]
  11. ATP signaling is crucial for communication from taste buds to gustatory nerves. Finger, T.E., Danilova, V., Barrows, J., Bartel, D.L., Vigers, A.J., Stone, L., Hellekant, G., Kinnamon, S.C. Science (2005) [Pubmed]
  12. Essential function of oncostatin m in nociceptive neurons of dorsal root ganglia. Morikawa, Y., Tamura, S., Minehata, K., Donovan, P.J., Miyajima, A., Senba, E. J. Neurosci. (2004) [Pubmed]
  13. Changes in P2X receptor responses of sensory neurons from P2X3-deficient mice. Zhong, Y., Dunn, P.M., Bardini, M., Ford, A.P., Cockayne, D.A., Burnstock, G. Eur. J. Neurosci. (2001) [Pubmed]
  14. Delayed upregulation of ATP P2X3 receptors of trigeminal sensory neurons by calcitonin gene-related peptide. Fabbretti, E., D'Arco, M., Fabbro, A., Simonetti, M., Nistri, A., Giniatullin, R. J. Neurosci. (2006) [Pubmed]
WikiGenes - Universities