The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

ALOX12  -  arachidonate 12-lipoxygenase

Homo sapiens

Synonyms: 12-LOX, 12LO, 12S-LOX, 12S-lipoxygenase, Arachidonate 12-lipoxygenase, 12S-type, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ALOX12


Psychiatry related information on ALOX12


High impact information on ALOX12

  • The very limited current data on the role of lipoxygenase (LOX) metabolism in tumorigenesis suggests that the oxidative metabolism of linoleic and arachidonic acid possibly shifts from producing antitumorigenic 15-LOX-1 and 15-LOX-2 products to producing protumorigenic 5-LOX and 12-LOX products [6].
  • Here, using an antisense oligonucleotide-based strategy, we show that blockade of 12-LOX expression prevents both A beta-induced apoptosis and overexpression of c-Jun, a factor required for the apoptotic process, in cortical neurons [7].
  • The results show that 12-LOX regulates cell survival and apoptosis by affecting the expression and localization of the vitronectin receptors, alpha(v)beta(3) and alpha(v)beta(5), in two cancer cell lines [8].
  • Furthermore, Baicalein or N-benzyl-N-hydroxy-5-phenylpentamide, specific 12-LOX inhibitors, significantly decreased alpha(v)beta(5)-mediated adhesion and survival in 12-LOX-overexpressing cells [8].
  • PC3 cells transfected with 12-LOX displayed increased alpha(v)beta(3) and alpha(v)beta(5) integrin expression, whereas other integrins were unaltered [8].

Chemical compound and disease context of ALOX12


Biological context of ALOX12

  • RESULTS: Moderate evidence of association was found between spine BMD and six SNPs in the ALOX12 gene in both men and women (p = 0.0052-0.050) [13].
  • Furthermore, the most common SNP haplotype in ALOX12 showed evidence of significant association with high spine BMD in men (p = 0.0083), whereas the second most common haplotype was associated with high spine BMD in women (p = 0.0081) [13].
  • Linkage analysis assigned S0601 distal to ALOX12 on SSC12, providing further evidence for the conservation of synteny between HSA17 and SSC12 [14].
  • Arachidonate 12-lipoxygenase purified from porcine leukocytes and human platelets, 15-lipoxygenase from rabbit reticulocytes and fatty acid cyclooxygenase from bovine vesicular glands were inhibited by the compound only at higher concentrations (IC50 = 2.3, 11, 5.2 and 2.5 microM, respectively) [15].
  • The apoptotic effect of BMD188 in prostate cancer cells does not depend on cell cycle, p53 status, or its purported target, arachidonate 12-lipoxygenase, but does require caspase activation and seems to involve mitochondria [16].

Anatomical context of ALOX12


Associations of ALOX12 with chemical compounds


Other interactions of ALOX12

  • These targets include procarcinogenic lipoxygenases (LOXs), including 5-, 8-, and 12-LOX, and anticarcinogenic LOXs, including 15-LOX-1 and possibly 15-LOX-2 [22].
  • 12-LOX expression was determined in normal human skin melanocytes and in melanocytes found in compound naevi, dysplastic naevi and melanomas using a platelet-type 12-LOX antibody with a diaminobenzidine immunoperoxidase system detection system and was quantified using the analysis software NIH Image 1.62 [4].
  • Also, following GPVI activation, 12-LOX co-immunoprecipitates with both cytosolic and secretory PLA2 (sPLA2) [23].
  • Subsequently, it is found that the levels of COX-2 and/or 12-LOX are frequently increased in various cancers [24].
  • Out of six polymorphisms in the coding region of ALOX12, only R261Q determined a nonconservative amino-acid change and was evaluated by polymerase chain reaction and restriction digestion [10].

Analytical, diagnostic and therapeutic context of ALOX12


  1. Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening. Tang, K., Finley, R.L., Nie, D., Honn, K.V. Biochemistry (2000) [Pubmed]
  2. Expression of lipoxygenase in human prostate cancer and growth reduction by its inhibitors. Matsuyama, M., Yoshimura, R., Mitsuhashi, M., Hase, T., Tsuchida, K., Takemoto, Y., Kawahito, Y., Sano, H., Nakatani, T. Int. J. Oncol. (2004) [Pubmed]
  3. Mechanisms of free-radical induction in relation to fenretinide-induced apoptosis of neuroblastoma. Lovat, P.E., Ranalli, M., Corazzari, M., Raffaghello, L., Pearson, A.D., Ponzoni, M., Piacentini, M., Melino, G., Redfern, C.P. J. Cell. Biochem. (2003) [Pubmed]
  4. Expression of 12-lipoxygenase as a biomarker for melanoma carcinogenesis. Winer, I., Normolle, D.P., Shureiqi, I., Sondak, V.K., Johnson, T., Su, L., Brenner, D.E. Melanoma Res. (2002) [Pubmed]
  5. Association of a new polymorphism in ALOX12 gene with bipolar disorder. Fridman, C., Ojopi, E.P., Gregório, S.P., Ikenaga, E.H., Moreno, D.H., Demetrio, F.N., Guimarães, P.E., Vallada, H.P., Gattaz, W.F., Dias Neto, E. European archives of psychiatry and clinical neuroscience. (2003) [Pubmed]
  6. The critical role of 15-lipoxygenase-1 in colorectal epithelial cell terminal differentiation and tumorigenesis. Shureiqi, I., Wu, Y., Chen, D., Yang, X.L., Guan, B., Morris, J.S., Yang, P., Newman, R.A., Broaddus, R., Hamilton, S.R., Lynch, P., Levin, B., Fischer, S.M., Lippman, S.M. Cancer Res. (2005) [Pubmed]
  7. Blockade of 12-lipoxygenase expression protects cortical neurons from apoptosis induced by beta-amyloid peptide. Lebeau, A., Terro, F., Rostene, W., Pelaprat, D. Cell Death Differ. (2004) [Pubmed]
  8. Overexpression of platelet-type 12-lipoxygenase promotes tumor cell survival by enhancing alpha(v)beta(3) and alpha(v)beta(5) integrin expression. Pidgeon, G.P., Tang, K., Cai, Y.L., Piasentin, E., Honn, K.V. Cancer Res. (2003) [Pubmed]
  9. Increased metastatic potential in human prostate carcinoma cells by overexpression of arachidonate 12-lipoxygenase. Nie, D., Nemeth, J., Qiao, Y., Zacharek, A., Li, L., Hanna, K., Tang, K., Hillman, G.G., Cher, M.L., Grignon, D.J., Honn, K.V. Clin. Exp. Metastasis (2003) [Pubmed]
  10. A coding polymorphism in the 12-lipoxygenase gene is associated to essential hypertension and urinary 12(S)-HETE. Quintana, L.F., Guzmán, B., Collado, S., Clària, J., Poch, E. Kidney Int. (2006) [Pubmed]
  11. Mechanisms controlling cell cycle arrest and induction of apoptosis after 12-lipoxygenase inhibition in prostate cancer cells. Pidgeon, G.P., Kandouz, M., Meram, A., Honn, K.V. Cancer Res. (2002) [Pubmed]
  12. Lipoxygenase inhibition induced apoptosis, morphological changes, and carbonic anhydrase expression in human pancreatic cancer cells. Ding, X.Z., Kuszynski, C.A., El-Metwally, T.H., Adrian, T.E. Biochem. Biophys. Res. Commun. (1999) [Pubmed]
  13. Human ALOX12, but Not ALOX15, Is Associated With BMD in White Men and Women. Ichikawa, S., Koller, D.L., Johnson, M.L., Lai, D., Xuei, X., Edenberg, H.J., Klein, R.F., Orwoll, E.S., Hui, S.L., Foroud, T.M., Peacock, M., Econs, M.J. J. Bone Miner. Res. (2006) [Pubmed]
  14. Genetic and physical mapping, expression analysis and partial sequence of porcine PER1. Skinner, T.M., Lopez-Corrales, N.L., Anderson, S.I., Loudon, A.S., Lowden, S., Haley, C.S., Archibald, A.L. Cytogenet. Cell Genet. (2001) [Pubmed]
  15. A prenylflavone, artonin E, as arachidonate 5-lipoxygenase inhibitor. Reddy, G.R., Ueda, N., Hada, T., Sackeyfio, A.C., Yamamoto, S., Hano, Y., Aida, M., Nomura, T. Biochem. Pharmacol. (1991) [Pubmed]
  16. Apoptosis induction by a novel anti-prostate cancer compound, BMD188 (a fatty acid-containing hydroxamic acid), requires the mitochondrial respiratory chain. Joshi, B., Li, L., Taffe, B.G., Zhu, Z., Wahl, S., Tian, H., Ben-Josef, E., Taylor, J.D., Porter, A.T., Tang, D.G. Cancer Res. (1999) [Pubmed]
  17. Immunocytochemical localization of platelet-type arachidonate 12-lipoxygenase in mouse blood cells. Nakamura, M., Ueda, N., Kishimoto, K., Yoshimoto, T., Yamamoto, S., Ishimura, K. J. Histochem. Cytochem. (1995) [Pubmed]
  18. Subcellular localization of arachidonate 12-lipoxygenase and morphological effect of its overexpression on murine keratinocytes. Nakamura, M., Yamamoto, S., Ishimura, K. Cell Tissue Res. (1997) [Pubmed]
  19. Oxidative modification of human lipoproteins by lipoxygenases of different positional specificities. Kühn, H., Belkner, J., Suzuki, H., Yamamoto, S. J. Lipid Res. (1994) [Pubmed]
  20. Structure and chromosomal localization of human arachidonate 12-lipoxygenase gene. Yoshimoto, T., Arakawa, T., Hada, T., Yamamoto, S., Takahashi, E. J. Biol. Chem. (1992) [Pubmed]
  21. Specific monocyte adhesion to endothelial cells induced by oxidized phospholipids involves activation of cPLA2 and lipoxygenase. Huber, J., Fürnkranz, A., Bochkov, V.N., Patricia, M.K., Lee, H., Hedrick, C.C., Berliner, J.A., Binder, B.R., Leitinger, N. J. Lipid Res. (2006) [Pubmed]
  22. Lipoxygenase modulation to reverse carcinogenesis. Shureiqi, I., Lippman, S.M. Cancer Res. (2001) [Pubmed]
  23. Interactions of 12-lipoxygenase with phospholipase A2 isoforms following platelet activation through the glycoprotein VI collagen receptor. Coffey, M.J., Coles, B., Locke, M., Bermudez-Fajardo, A., Williams, P.C., Jarvis, G.E., O'donnell, V.B. FEBS Lett. (2004) [Pubmed]
  24. Eicosanoid regulation of angiogenesis in tumors. Nie, D., Honn, K.V. Semin. Thromb. Hemost. (2004) [Pubmed]
  25. Molecular cloning and expression of human arachidonate 12-lipoxygenase. Yoshimoto, T., Yamamoto, Y., Arakawa, T., Suzuki, H., Yamamoto, S., Yokoyama, C., Tanabe, T., Toh, H. Biochem. Biophys. Res. Commun. (1990) [Pubmed]
  26. Molecular identification, localization and function of platelet-type 12-lipoxygenase in human melanoma progression, under experimental and clinical conditions. Rásó, E., Döme, B., Somlai, B., Zacharek, A., Hagmann, W., Honn, K.V., Tímár, J. Melanoma Res. (2004) [Pubmed]
  27. Aberrant expression of 5-lipoxygenase-activating protein (5-LOXAP) has prognostic and survival significance in patients with breast cancer. Jiang, W.G., Douglas-Jones, A.G., Mansel, R.E. Prostaglandins Leukot. Essent. Fatty Acids (2006) [Pubmed]
WikiGenes - Universities