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Gene Review

MMP13  -  matrix metallopeptidase 13 (collagenase 3)

Homo sapiens

Synonyms: CLG3, Collagenase 3, MANDP1, MMP-13, Matrix metalloproteinase-13
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Disease relevance of MMP13


High impact information on MMP13

  • We show that a missense mutation of MMP13 causes the Missouri type of human spondyloepimetaphyseal dysplasia (SEMD(MO)), an autosomal dominant disorder characterized by defective growth and modeling of vertebrae and long bones [6].
  • Histological evaluation of joint sections revealed no difference in the extent of synovial inflammation, its cellular composition (except for the lack of osteoclasts), and the expression of matrix metalloprotein-ase-3 (MMP-3) and MMP-13 [7].
  • These data suggest that collagenase(s) produced by chondrocytes is (are) involved in the cleavage and denaturation of type II collagen in articular cartilage, that this is increased in OA, and that MMP-13 may play a significant role in this process [8].
  • Experiments with intact type II collagen as well as a synthetic peptide suggested that MMP-13 cleaved type II collagen at the same bond as MMP-1, but this was then followed by a secondary cleavage that removed three amino acids from the 1/4 fragment amino terminus [9].
  • Degenerate reverse transcription polymerase chain reaction experiments, Northern blot analysis, and direct immunodetection have now provided evidence that collagenase-3 (MMP-13), an enzyme recently cloned from human breast carcinoma, is expressed by chondrocytes in human osteoarthritic cartilage [9].

Chemical compound and disease context of MMP13


Biological context of MMP13


Anatomical context of MMP13

  • MMP13 promoter polymorphism is associated with atherosclerosis in the abdominal aorta of young black males [16].
  • Expression of mRNAs encoding for growth factors, ECM molecules, and MMP13 in mono-cultures and co-cultures of human periodontal ligament fibroblasts and alveolar bone cells [19].
  • Additionally, we have shown that in cartilage the median level of steady-state mRNA for MMP13 is approximately 20-fold higher than MMP28 and approximately 1,500-fold higher than ADAMTS16, with expression of this latter gene approximately 150-fold higher in synovium than cartilage [20].
  • RESULTS: MMP-13 was detected in fibroblast- and macrophage-like mononuclear cells in the synovial lining and stroma and in vascular endothelial cells [3].
  • Our data provide direct evidence for the role of MMP-13 as a potent invasion proteinase, which alone can enhance the ability of malignant cells to penetrate through both basement membrane and fibrillar collagen [21].

Associations of MMP13 with chemical compounds


Physical interactions of MMP13

  • Reconstituted MMP-13/alpha 2M complex was unable to degrade collagen (MW 300,000) in contrast to the low-molecular-weight fluorogenic substrate (MW < 1500) [26].

Enzymatic interactions of MMP13

  • The results show that MMP-13 cleaves aggrecan in the IGD at the same site (..PEN314-FFG..) identified for other members of the MMP family, and also at a novel site ..VKP384-VFE.. not previously observed for other proteinases [27].
  • We showed, for the first time, that MMP-13 can degrade members from two classes of the SLRP family, and identified the site at which biglycan is cleaved by MMP-13 [28].

Regulatory relationships of MMP13


Other interactions of MMP13


Analytical, diagnostic and therapeutic context of MMP13


  1. Structural analysis and promoter characterization of the human collagenase-3 gene (MMP13). Pendás, A.M., Balbín, M., Llano, E., Jiménez, M.G., López-Otín, C. Genomics (1997) [Pubmed]
  2. Differentially expressed genes in neurofibromatosis 1-associated neurofibromas and malignant peripheral nerve sheath tumors. Holtkamp, N., Mautner, V.F., Friedrich, R.E., Harder, A., Hartmann, C., Theallier-Janko, A., Hoffmann, K.T., von Deimling, A. Acta Neuropathol. (2004) [Pubmed]
  3. Matrix metalloproteinase 13 (collagenase 3) in human rheumatoid synovium. Lindy, O., Konttinen, Y.T., Sorsa, T., Ding, Y., Santavirta, S., Ceponis, A., López-Otín, C. Arthritis Rheum. (1997) [Pubmed]
  4. Biochemical characterization of human collagenase-3. Knäuper, V., López-Otin, C., Smith, B., Knight, G., Murphy, G. J. Biol. Chem. (1996) [Pubmed]
  5. Wild type and mutant p53 differentially regulate the gene expression of human collagenase-3 (hMMP-13). Sun, Y., Cheung, J.M., Martel-Pelletier, J., Pelletier, J.P., Wenger, L., Altman, R.D., Howell, D.S., Cheung, H.S. J. Biol. Chem. (2000) [Pubmed]
  6. MMP13 mutation causes spondyloepimetaphyseal dysplasia, Missouri type (SEMD(MO). Kennedy, A.M., Inada, M., Krane, S.M., Christie, P.T., Harding, B., López-Otín, C., Sánchez, L.M., Pannett, A.A., Dearlove, A., Hartley, C., Byrne, M.H., Reed, A.A., Nesbit, M.A., Whyte, M.P., Thakker, R.V. J. Clin. Invest. (2005) [Pubmed]
  7. Osteoclasts are essential for TNF-alpha-mediated joint destruction. Redlich, K., Hayer, S., Ricci, R., David, J.P., Tohidast-Akrad, M., Kollias, G., Steiner, G., Smolen, J.S., Wagner, E.F., Schett, G. J. Clin. Invest. (2002) [Pubmed]
  8. Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage. Billinghurst, R.C., Dahlberg, L., Ionescu, M., Reiner, A., Bourne, R., Rorabeck, C., Mitchell, P., Hambor, J., Diekmann, O., Tschesche, H., Chen, J., Van Wart, H., Poole, A.R. J. Clin. Invest. (1997) [Pubmed]
  9. Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase-13 from human osteoarthritic cartilage. Mitchell, P.G., Magna, H.A., Reeves, L.M., Lopresti-Morrow, L.L., Yocum, S.A., Rosner, P.J., Geoghegan, K.F., Hambor, J.E. J. Clin. Invest. (1996) [Pubmed]
  10. Histamine stimulates matrix metalloproteinase-3 and -13 production by human articular chondrocytes in vitro. Tetlow, L.C., Woolley, D.E. Ann. Rheum. Dis. (2002) [Pubmed]
  11. Tretinoin reverses upregulation of matrix metalloproteinase-13 in human keloid-derived fibroblasts. Uchida, G., Yoshimura, K., Kitano, Y., Okazaki, M., Harii, K. Exp. Dermatol. (2003) [Pubmed]
  12. Effect of estrogen on the expression of matrix metalloproteinase (MMP)-1, MMP-3, and MMP-13 and tissue inhibitor of metalloproternase-1 in osteoarthritis chondrocytes. Lee, Y.J., Lee, E.B., Kwon, Y.E., Lee, J.J., Cho, W.S., Kim, H.A., Song, Y.W. Rheumatol. Int. (2003) [Pubmed]
  13. Alpha v beta 6 integrin down-regulates the MMP-13 expression in oral squamous cell carcinoma cells. Ylipalosaari, M., Thomas, G.J., Nystrom, M., Salhimi, S., Marshall, J.F., Huotari, V., Tervahartiala, T., Sorsa, T., Salo, T. Exp. Cell Res. (2005) [Pubmed]
  14. The regulation of human MMP-13 by licofelone, an inhibitor of cyclo-oxygenases and 5-lipoxygenase, in human osteoarthritic chondrocytes is mediated by the inhibition of the p38 MAP kinase signalling pathway. Boileau, C., Pelletier, J.P., Tardif, G., Fahmi, H., Laufer, S., Lavigne, M., Martel-Pelletier, J. Ann. Rheum. Dis. (2005) [Pubmed]
  15. In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis. Sekiya, I., Vuoristo, J.T., Larson, B.L., Prockop, D.J. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  16. MMP13 promoter polymorphism is associated with atherosclerosis in the abdominal aorta of young black males. Yoon, S., Kuivaniemi, H., Gatalica, Z., Olson, J.M., Butticè, G., Ye, S., Norris, B.A., Malcom, G.T., Strong, J.P., Tromp, G. Matrix Biol. (2002) [Pubmed]
  17. The correlation between difference in foreign body reaction between implant locations and cytokine and MMP expression. Luttikhuizen, D.T., van Amerongen, M.J., de Feijter, P.C., Petersen, A.H., Harmsen, M.C., van Luyn, M.J. Biomaterials (2006) [Pubmed]
  18. Inhibitory effects of insulin-like growth factor-1 and osteogenic protein-1 on fibronectin fragment- and interleukin-1beta-stimulated matrix metalloproteinase-13 expression in human chondrocytes. Im, H.J., Pacione, C., Chubinskaya, S., Van Wijnen, A.J., Sun, Y., Loeser, R.F. J. Biol. Chem. (2003) [Pubmed]
  19. Expression of mRNAs encoding for growth factors, ECM molecules, and MMP13 in mono-cultures and co-cultures of human periodontal ligament fibroblasts and alveolar bone cells. Winter, S., Kohl, A., Huppertz, A., Herold-Mende, C., Wiest, T., Komposch, G., Tomakidi, P. Cell Tissue Res. (2005) [Pubmed]
  20. Expression profiling of metalloproteinases and their inhibitors in synovium and cartilage. Davidson, R.K., Waters, J.G., Kevorkian, L., Darrah, C., Cooper, A., Donell, S.T., Clark, I.M. Arthritis Res. Ther. (2006) [Pubmed]
  21. Expression of collagenase-3 (MMP-13) enhances invasion of human fibrosarcoma HT-1080 cells. Ala-Aho, R., Johansson, N., Baker, A.H., Kähäri, V.M. Int. J. Cancer (2002) [Pubmed]
  22. Dose-dependent effects of corticosteroids on the expression of matrix-related genes in normal and cytokine-treated articular chondrocytes. Richardson, D.W., Dodge, G.R. Inflamm. Res. (2003) [Pubmed]
  23. Retinoic acid and oncostatin M combine to promote cartilage degradation via matrix metalloproteinase-13 expression in bovine but not human chondrocytes. Shingleton, W.D., Jones, D., Xu, X., Cawston, T.E., Rowan, A.D. Rheumatology (Oxford, England) (2006) [Pubmed]
  24. Inhibition of interleukin-1-stimulated MAP kinases, activating protein-1 (AP-1) and nuclear factor kappa B (NF-kappa B) transcription factors down-regulates matrix metalloproteinase gene expression in articular chondrocytes. Liacini, A., Sylvester, J., Li, W.Q., Zafarullah, M. Matrix Biol. (2002) [Pubmed]
  25. CCAAT/enhancer binding protein beta mediates expression of matrix metalloproteinase 13 in human articular chondrocytes in inflammatory arthritis. Hayashida, M., Okazaki, K., Fukushi, J., Sakamoto, A., Iwamoto, Y. Arthritis Rheum. (2009) [Pubmed]
  26. Fluorogenic MMP activity assay for plasma including MMPs complexed to alpha 2-macroglobulin. Beekman, B., Drijfhout, J.W., Ronday, H.K., TeKoppele, J.M. Ann. N. Y. Acad. Sci. (1999) [Pubmed]
  27. Degradation of cartilage aggrecan by collagenase-3 (MMP-13). Fosang, A.J., Last, K., Knäuper, V., Murphy, G., Neame, P.J. FEBS Lett. (1996) [Pubmed]
  28. Degradation of small leucine-rich repeat proteoglycans by matrix metalloprotease-13: identification of a new biglycan cleavage site. Monfort, J., Tardif, G., Reboul, P., Mineau, F., Roughley, P., Pelletier, J.P., Martel-Pelletier, J. Arthritis Res. Ther. (2006) [Pubmed]
  29. Differential regulation of cytokine-induced MMP-1 and MMP-13 expression by p38 kinase inhibitors in human chondrosarcoma cells: potential role of Runx2 in mediating p38 effects. Pei, Y., Harvey, A., Yu, X.P., Chandrasekhar, S., Thirunavukkarasu, K. Osteoarthr. Cartil. (2006) [Pubmed]
  30. Adenoviral delivery of p53 gene suppresses expression of collagenase-3 (MMP-13) in squamous carcinoma cells. Ala-aho, R., Grénman, R., Seth, P., Kähäri, V.M. Oncogene (2002) [Pubmed]
  31. Vascular expression of matrix metalloproteinase-13 (collagenase-3) in basal cell carcinoma. Hattori, Y., Nerusu, K.C., Bhagavathula, N., Brennan, M., Hattori, N., Murphy, H.S., Su, L.D., Wang, T.S., Johnson, T.M., Varani, J. Exp. Mol. Pathol. (2003) [Pubmed]
  32. Transforming growth factor-beta induces collagenase-3 expression by human gingival fibroblasts via p38 mitogen-activated protein kinase. Ravanti, L., Häkkinen, L., Larjava, H., Saarialho-Kere, U., Foschi, M., Han, J., Kähäri, V.M. J. Biol. Chem. (1999) [Pubmed]
  33. Production of cytokines, vascular endothelial growth factor, matrix metalloproteinases, and tissue inhibitor of metalloproteinases 1 by tenosynovium demonstrates its potential for tendon destruction in rheumatoid arthritis. Jain, A., Nanchahal, J., Troeberg, L., Green, P., Brennan, F. Arthritis Rheum. (2001) [Pubmed]
  34. Oncostatin M induces angiogenesis and cartilage degradation in rheumatoid arthritis synovial tissue and human cartilage cocultures. Fearon, U., Mullan, R., Markham, T., Connolly, M., Sullivan, S., Poole, A.R., Fitzgerald, O., Bresnihan, B., Veale, D.J. Arthritis Rheum. (2006) [Pubmed]
  35. Release of matrix metalloproteinases following alcohol septal ablation in hypertrophic obstructive cardiomyopathy. Bradham, W.S., Gunasinghe, H., Holder, J.R., Multani, M., Killip, D., Anderson, M., Meyer, D., Spencer, W.H., Torre-Amione, G., Spinale, F.G. J. Am. Coll. Cardiol. (2002) [Pubmed]
  36. Differential expression of matrix metalloproteinases and their inhibitors in non-small cell lung cancer. Thomas, P., Khokha, R., Shepherd, F.A., Feld, R., Tsao, M.S. J. Pathol. (2000) [Pubmed]
  37. Translational repression of human matrix metalloproteinases-13 by an alternatively spliced form of T-cell-restricted intracellular antigen-related protein (TIAR). Yu, Q., Cok, S.J., Zeng, C., Morrison, A.R. J. Biol. Chem. (2003) [Pubmed]
  38. Endothelin 1 promotes osteoarthritic cartilage degradation via matrix metalloprotease 1 and matrix metalloprotease 13 induction. Roy-Beaudry, M., Martel-Pelletier, J., Pelletier, J.P., M'Barek, K.N., Christgau, S., Shipkolye, F., Moldovan, F. Arthritis Rheum. (2003) [Pubmed]
  39. Reduction of cytokine-induced expression and activity of MMP-1 and MMP-13 by mechanical strain in MH7A rheumatoid synovial cells. Sun, H.B., Yokota, H. Matrix Biol. (2002) [Pubmed]
  40. Matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase mRNA levels are specifically altered in torn rotator cuff tendons. Lo, I.K., Marchuk, L.L., Hollinshead, R., Hart, D.A., Frank, C.B. The American journal of sports medicine. (2004) [Pubmed]
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