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Gene Review

PNP  -  purine nucleoside phosphorylase

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Disease relevance of NP

  • A competitive ELISA (C-ELISA) using monoclonal antibodies (mAbs) which bind to the nucleo-protein (NP) of rinderpest virus (RPV) for detection of RPV antibodies in cattle and small ruminant sera is described [1].
  • A structural genomics comparison of purine nucleoside phosphorylases (PNPs) indicated that the enzyme encoded by Mycobacterium tuberculosis (TB-PNP) resembles the mammalian trimeric structure rather than the bacterial hexameric PNPs [2].
  • With the calf enzyme, the pseudo-first-order rate constants (Vmax/K(m)) for phosphorolysis of RibfAde and RibfHyp are 3% and 13%, respectively, that for phosphorolysis of Ino, while for E. coli PNP the corresponding values are 22% and 30%, respectively [3].

High impact information on NP


Chemical compound and disease context of NP


Biological context of NP


Anatomical context of NP


Associations of NP with chemical compounds

  • Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with hypoxanthine at 2.15 A resolution [16].
  • The crystal structure of the binary complex of trimeric purine nucleoside phosphorylase (PNP) from calf spleen with the acyclic nucleoside phosphonate inhibitor 2,6-diamino-(S)-9-[2-(phosphonomethoxy)propyl]purine ((S)-PMPDAP) is determined at 2.3A resolution in space group P2(1)2(1)2(1) [17].
  • In addition, a well-ordered water molecule is found in the PNP active site when purine base or nucleoside is also present [8].
  • The preparation of phosphate-free crystals of PNP has allowed us to analyze several novel complexes, including the ternary complex of PNP, purine base, and ribose 1-phosphate and of the completely unbound PNP [8].
  • PNP catalyzes the reversible phosphorolysis of 2'-deoxypurine ribonucleosides to the free bases and 2-deoxyribose 1-phosphate [8].

Other interactions of NP


Analytical, diagnostic and therapeutic context of NP


  1. A competitive ELISA using anti-N monoclonal antibodies for specific detection of rinderpest antibodies in cattle and small ruminants. Libeau, G., Diallo, A., Calvez, D., Lefèvre, P.C. Vet. Microbiol. (1992) [Pubmed]
  2. Structures of purine nucleoside phosphorylase from Mycobacterium tuberculosis in complexes with immucillin-H and its pieces. Shi, W., Basso, L.A., Santos, D.S., Tyler, P.C., Furneaux, R.H., Blanchard, J.S., Almo, S.C., Schramm, V.L. Biochemistry (2001) [Pubmed]
  3. Kinetics of phosphorolysis of 3-(beta-D-ribofuranosyl)adenine and 3-(beta-D-ribofuranosyl)hypoxanthine, non-conventional substrates of purine-nucleoside phosphorylase. Bzowska, A., Kulikowska, E., Poopeiko, N.E., Shugar, D. Eur. J. Biochem. (1996) [Pubmed]
  4. Structural and mutational analysis of substrate complexation by anthranilate phosphoribosyltransferase from Sulfolobus solfataricus. Marino, M., Deuss, M., Svergun, D.I., Konarev, P.V., Sterner, R., Mayans, O. J. Biol. Chem. (2006) [Pubmed]
  5. Purine nucleoside phosphorylase. Kinetic mechanism of the enzyme from calf spleen. Porter, D.J. J. Biol. Chem. (1992) [Pubmed]
  6. Evidence for a phosphoryl-enzyme intermediate in phosphate ester hydrolysis by purple acid phosphatase from bovine spleen. Vincent, J.B., Crowder, M.W., Averill, B.A. J. Biol. Chem. (1991) [Pubmed]
  7. Purine nucleoside phosphorylase. Allosteric regulation of a dissociating enzyme. Ropp, P.A., Traut, T.W. J. Biol. Chem. (1991) [Pubmed]
  8. Calf spleen purine nucleoside phosphorylase complexed with substrates and substrate analogues. Mao, C., Cook, W.J., Zhou, M., Federov, A.A., Almo, S.C., Ealick, S.E. Biochemistry (1998) [Pubmed]
  9. Calf spleen purine nucleoside phosphorylase: purification, sequence and crystal structure of its complex with an N(7)-acycloguanosine inhibitor. Bzowska, A., Luić, M., Schröder, W., Shugar, D., Saenger, W., Koellner, G. FEBS Lett. (1995) [Pubmed]
  10. Kinetics of utilization of Sendai virus RNA and protein in the process of virion assembly. Famulari, N.G., Fleissner, E. J. Virol. (1976) [Pubmed]
  11. Note on a third allele in the erythrocytic NP system of cattle. Ansay, M. Animal blood groups and biochemical genetics. (1975) [Pubmed]
  12. A MTT-linked spot test for the detection of 'silent' phenotypes of purine nucleoside phosphorylase (NP) in cattle erythrocytes. Ansay, M., Baldewijns-Rouma, V., Smith, J.E. Animal blood groups and biochemical genetics. (1975) [Pubmed]
  13. Structure-based design of inhibitors of purine nucleoside phosphorylase. 1. 9-(arylmethyl) derivatives of 9-deazaguanine. Montgomery, J.A., Niwas, S., Rose, J.D., Secrist, J.A., Babu, Y.S., Bugg, C.E., Erion, M.D., Guida, W.C., Ealick, S.E. J. Med. Chem. (1993) [Pubmed]
  14. Characterization of membrane-bound and solubilized high-affinity binding sites for 5'-N-ethylcarboxamido[3H]adenosine from bovine cerebral cortex. Lorenzen, A., Nitsch-Kirsch, M., Vogt, H., Schwabe, U. J. Neurochem. (1993) [Pubmed]
  15. Purification and characterization of a purine-nucleoside phosphorylase from bovine thyroid. Moyer, T.P., Fischer, A.G. Arch. Biochem. Biophys. (1976) [Pubmed]
  16. Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with hypoxanthine at 2.15 A resolution. Koellner, G., Luić, M., Shugar, D., Saenger, W., Bzowska, A. J. Mol. Biol. (1997) [Pubmed]
  17. Crystal structure of calf spleen purine nucleoside phosphorylase with two full trimers in the asymmetric unit: important implications for the mechanism of catalysis. Bzowska, A., Koellner, G., Wielgus-Kutrowska, B., Stroh, A., Raszewski, G., Holý, A., Steiner, T., Frank, J. J. Mol. Biol. (2004) [Pubmed]
  18. Determination of inorganic phosphate by flow injection method with immobilized enzymes and chemiluminescence detection. Kawasaki, H., Sato, K., Ogawa, J., Hasegawa, Y., Yuki, H. Anal. Biochem. (1989) [Pubmed]
  19. Comparative gene frequencies between the Canchim breed of Brazil beef cattle and their foundation breeds. Panepucci, L., Vicente, V. Comp. Biochem. Physiol., B (1991) [Pubmed]
  20. Atomic dissection of the hydrogen bond network for transition-state analogue binding to purine nucleoside phosphorylase. Kicska, G.A., Tyler, P.C., Evans, G.B., Furneaux, R.H., Shi, W., Fedorov, A., Lewandowicz, A., Cahill, S.M., Almo, S.C., Schramm, V.L. Biochemistry (2002) [Pubmed]
  21. Oligomeric structure of mammalian purine nucleoside phosphorylase in solution determined by analytical ultracentrifugation. Behlke, J., Koellner, G., Bzowska, A. Z. Naturforsch., C, J. Biosci. (2005) [Pubmed]
  22. An improved technique for isolated perfusion of rat livers and an evaluation of perfusates. Mischinger, H.J., Walsh, T.R., Liu, T., Rao, P.N., Rubin, R., Nakamura, K., Todo, S., Starzl, T.E. J. Surg. Res. (1992) [Pubmed]
  23. Interactions of calf spleen purine nucleoside phosphorylase with antiviral acyclic nucleoside phosphonate inhibitors: kinetics and emission studies. Wierzchowski, J., Kulikowska, E., Bzowska, A., Holy, A., Magnowska, L., Shugar, D. Nucleosides Nucleotides (1999) [Pubmed]
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